شناسنامه علمی شماره
نویسندگان
1 دانشیار گروه بهداشت و کنترل کیفی مواد غذایی دانشکده دامپزشکی دانشگاه شهرکرد
2 دانشجوی دانشکده دامپزشکی دانشگاه شهرکرد
3 کارشناس آزمایشگاه گروه بهداشت و کنترل کیفی مواد غذایی دانشگاه شهرکرد
چکیده
عنوان مقاله [English]
نویسندگان [English]
Data describing the microbiological status of a country’s meat products can be of great value: in acting as a de facto validation of the country’s regulatory systems, as a baseline for setting performance standards and in supporting risk assessment. O26, O45, O91, O103, O111, O113, O121, O128, O145, and O157 are the most common serotypes of enterohemorrhagic Escherichia coli associated with human disease. Considering the importance of what mentioned and the potential ability of sheep meat to transmit enterohemorrhagic E. coli to humans, the present study was conducted to evaluate sheep meat contamination to E. coli and E. coli O157, O26 and O128 serotypes in Shahrekord by PCR. Altogether 135 samples of sheep carcasses were collected from Jooneghan abattoir, Chaharmahalvabakhtiari, Iran. Samples enriched in Tryptone Soya Broth (TSB). Then, incubated broth culture was streaked onto Sorbitol MacConkey agar and MacConkey agar as selective plating media. Suspected colonies were tested by polymerase chain reaction. The contamination rate in sheep carcasses with E. coli was 50.37% (68 out of 135). 1.48% (2 out of 135) of the samples was contaminated with E. coli O128. E. coli O157 and O26 were not found in any samples. Present study suggests E. coli O157 and O26 are not prevalent in the region.
)
