نوع مقاله : مقاله کامل
نویسندگان
1 گروه بهداشت مواد غذایی و آبزیان، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران
2 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Introduction: Linguatula serrata, commonly called the tongue worm, is a zoonotic parasite of the order Pentastomida. Its nymphs lodge in the internal organs of ruminants, causing lesions, organ condemnation, and economic losses in the livestock industry. Human infection may also occur through consumption of raw or undercooked tissues containing viable nymphs, emphasizing its veterinary and public health importance.
Objective: Increasing drug resistance and the harmful effects of chemical anthelmintics on health and the environment have driven interest in medicinal plants as alternative therapies. Nettle (Urtica dioica), a traditional herb with known bioactive compounds, was selected for evaluation. This study aimed to assess the lethal effects of its aqueous and alcoholic extracts against L. serrata nymphs under in vitro conditions.
Methods: Nymphs were isolated from mesenteric lymph nodes of slaughtered goats and exposed to four concentrations (10, 1, 0.1, and 0.01 mg/mL) of each extract for 24–96 hours. Ivermectin was used as the positive control and phosphate buffer as the negative control. Mortality was assessed by immobility and lack of reaction. LC₅₀ values were determined using probit analysis. Data were analyzed by one-way ANOVA and Duncan’s test, with p < 0.05 considered significant.
Results: The aqueous extract caused 100% mortality at 10 mg/mL within 48 hours, with an LC₅₀ of 0.964 mg/mL. The alcoholic extract produced 84% mortality after 96 hours at the same concentration (LC₅₀ = 1.188 mg/mL). Ivermectin induced 96–100% mortality within 12 hours at all concentrations (LC₅₀ = 0.316). The negative control showed no effect.
کلیدواژهها [English]
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