اثرات هم‌افزایی ایزوفرم‌های کوله‌سیستوکینین (CCK4 و CCK8s) با گلوتامات بر اخذ غذا در جوجه‌های تخمگذار

نوع مقاله : مقاله کامل

نویسندگان

1 گروه علوم پایه، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 دانشگاه تهران

3 استادیار جراحی دانشکده دامپزشکی دانشگاه علوم وتحقیقات تهران

چکیده

در مطالعات پیشین نقش کولهسیستوکینین )CCK )و گلوتامات به طور مستقل در کنرتل مرکزی مرصف غذا در پرندگان به اثبات رسیده
است. مطالعه حارض با هدف بررسی اثرات همافزایی CCK و گلوتامات بر رفتار تغذیهای در جوجههای تخمگذار انجام شد. به این منظور
چهار آزمایش طراحی گشت. در آزمایش اول به ترتیب محلول کنرتل، 4CCK( 1 نانومول(، گلوتامات )75 نانومول( و 4CCK + گلوتامات به
صورت داخل بطن مغزی )ICV )تزریق شد. آزمایش دوم مطابق با آزمایش اول انجام گرفت، با این تفاوت که گلوتامات با دوز مؤثر )300
نانومول( تزریق گشت. در آزمایش سوم محلول کنرتل، s8CCK( 0/125 نانومول(، گلوتامات )75 نانومول( و s8CCK + گلوتامات تجویز
شدند. آزمایش چهارم نیز مطابق با آزمایش سوم بوده و تنها دوزهای s8CCK( 1 نانومول( و گلوتامات )300 نانومول( تغییر پیدا کردند.
بر اساس نتایج بدست آمده تجویز دوزهای تحت اثر 4CCK و گلوتامات به طور جداگانه یا همزمان اثر معناداری بر اخذ غذای جوجهها
نداشت )0/05>p). تزریق دوز مؤثر گلوتامات منجر به کاهش معنی دار مرصف خوراک شد و تجویز همزمان 4CCK با گلوتامات تغییری
در این اثر هیپوفاژیک ایجاد ننمود )0/05 >p). تزریق دوزهای تحت اثر s8CCK و گلوتامات اثر معنیداری بر اخذ غذا نداشت )0/05>p)،
با این حال تجویز همزمان آنها دریافت غذا را تضعیف کرد )0/05<P). همچنین مشاهده شد که تزریق دوزهای مؤثر s8CCK و گلوتامات
به طور جداگانه دریافت غذا در جوجهها را کاهش داده و تجویز توأمان آنها موجب تقویت این اثر هیپوفاژیک شد )0/05<P). در نهایت،
به نظر می ً رسد احتامال یک اثر همافزایی میان s8CCK و گلوتامات در تنظیم اخذ غذا در جوجههای تخمگذار وجود داشته باشد

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Synergistic effects of cholecystokinin isoforms (CCK4 and CCK8s) with glutamate on food intake in laying chickens

نویسندگان [English]

  • Mohammad Jelokhani 1
  • Morteza zendehdel 2
  • Bita Vazir 1
  • Alireza jahandideh 3
1 Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 university of tehran
3 Islamic Azad University, Tehran, Iran. Veterinary Medicine
چکیده [English]

The present study was conducted with the aim of investigating the synergistic effects of CCK and glutamate on feeding behavior in layers. For this purpose. In the first experiment, the control solution, CCK4 (1 nmol), glutamate (75 nmol), and CCK4 + glutamate were injected intracerebroventricularly (ICV). The second experiment was performed according to the first experiment, with the difference that glutamate was injected with an effective dose (300 nmol). In the third experiment, the control solution, CCK8s (0.125 nmol), glutamate (75 nmol), and CCK8s + glutamate were administered. The fourth experiment was also the same as the third experiment and only the doses of CCK8s (1 nmol) and glutamate (300 nmol) were changed. According to the obtained results, the administration of doses under the effect of CCK4 and glutamate separately or simultaneously did not have a significant effect on the food intake of chickens (p<0.05). Injection of an effective dose of glutamate led to a significant decrease in feed intake, and simultaneous administration of CCK4 with glutamate did not change this hypophagic effect (p<0.05). Injection of sub-effective doses of CCK8s and glutamate had no significant effect on food intake (p<0.05), however, their simultaneous administration weakened food intake (p<0.05). Injection of effective doses of CCK8s and glutamate separately caused a significant decrease in food intake in chickens, and the administration of both of them strengthened this hypophagic effect (p<0.05). Finally, it seems that there is a synergistic effect between CCK8s and glutamate in the regulation of food intake in laying chickens.

کلیدواژه‌ها [English]

  • Cholecystokinin
  • Glutamate
  • Food intake
  • Layers

مراجع

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تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
دوره 37، شماره 1
فروردین 1403
صفحه 42-49

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سابقه مقاله

  • تاریخ دریافت: 01 شهریور 1402
  • تاریخ بازنگری: 17 شهریور 1402
  • تاریخ پذیرش: 17 شهریور 1402

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