واکاوی پروفایل بیان ژنی به منظور شناسایی ژن‌های کلیدی در مسیر لیپوژنز در جوجه گوشتی

نوع مقاله : مقاله کامل

نویسندگان

1 گروه علوم دامی ، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت، ایران.

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت، ایران

3 گروه علوم دامی، دانشکده کشاورزی، دانشگاه جیرفت، جیرفت ، ایران

چکیده

وجود چربی در لاشه جوجه‌های گوشتی یکی از چالش‌های اصلی در صنعت طیور می‌باشد. لیپوژنز کبدی از مراحل مهم مرتبط با متابولیسم چربی در جوجه‌های گوشتی است که توسط مجموعه ای از ژن ها کنترل می‌شود. پژوهش کنونی به هدف واکاوی پروفایل بیان ژنی برای شناسایی ژن‌های موثر در مسیر لیپوژنز انجام شد. ابتدا داده‌های پروفایل بیانی مربوط به سه بافت کبد، ماهیچه سینه و چربی شکمی از جوجه‌های گوشتی تغذیه شده با دو منبع مختلف انرژی دانلود شدند. سپس، آنالیز بیان ژن‌های مربوط به سه بافت ذکر شده با استفاده از نرم‌افزار تحت وب GEO2R انجام شد. بررسی مسیرهای زیستی با استفاده از پایگاه WebGestalt و بازسازی شبکه ژنی به کمک نرم‌افزار Cytoscape انجام شد. تجزیه و تحلیل نتایج حاصله نشان داد در دو بافت ماهیچه سینه و چربی شکمی تفاوت معنی‌داری در پروفایل بیانی در اثر تغدیه با دو جیره مختلف وجود ندارد اما در بافت کبد 975 ژن تفاوت معنی‌دار داشتند. براساس آنالیز شبکه و مسیر زیستی 10 ژن با بالاترین درجه در فعالیت‌های بیولوژیکی بیوسنتز اسید چرب، بیوسنتز اسیل کوآنزیم آ، متابولیسم اسید چرب و تجزیه اسید چرب نقش داشتند. از این‌رو این ژن‌ها می‌توانند بعنوان ژن‌های کلیدی کنترل کننده لیپوژنز کبدی اهمیت داشته باشند. بنابراین براساس یافته‌های به دست آمده در پژوهش کنونی، که حاصل برهم‌کنش بین تغذیه و ژنوم می‌باشد، می‌توان جیره‌های غذایی را برای پرورش طیور گوشتی پیشنهاد داد که از آنها به عنوان جیره مناسب، برای تولید گوشت کم چربی استفاده کرد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Analysis of gene expression profiles to identify key genes involved in the lipogenesis pathway in meat chickens

نویسندگان [English]

  • Fariba Mashayekhi 1
  • Mozhgan Mazhari 1
  • Omidali Esmaeilipour 1
  • Morteza Mokhtari 2
  • zahra Roudbari 3
1 Department of Animal Science, Faculty of Agriculture, University of Jiroft. Jiroft. Iran
2 Department of Animal Science, Faculty of Agriculture, JiroftUniversity, Jiroft, Iran
3 Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft,Iran
چکیده [English]

 The presence of fat in the carcasses of broilers is one of the main challenges in the poultry industry. Hepatic lipogenesis is an important step related to lipid metabolism in broilers that is controlled by a set of genes. The aim of the present study was to investigate the gene expression profile to identify genes that are effective in the lipogenesis pathway.
Materials and methods: In the first, the gene expression profiles related to three tissues of the liver, breast, and abdominal fat of broilers fed with two diets containing high starch, low fiber, low fat (LF), and low starch, high fiber, high fat (HF) were downloaded from GEO database. In the next step gene expression analysis related to the three tissue mentioned was performed by GEO2R web-based software. The study of biological pathways was performed by the WebGestalt tool and the gene network was reconstructed by Cytoscape software. 
Results: The analysis of the results showed that there was no significant difference in the expression profile due to feeding with two different diets in the breast muscle tissue and abdominal fat, but 975 genes were significantly different in the liver tissue. Based on a network and biological analysis, 10 genes with the highest degree were involved in the biological activities of fatty acid biosynthesis, acyl-coenzyme A biosynthesis, fatty acid metabolism, and fatty acid degradation.
Conclusion: Therefore, these genes can be important as key genes controlling hepatic lipogenesis. Therefore, based on our findings, which are the result of the interaction between nutrition and the genome, we can suggest diets for breeding poultry, which can be used as a suitable diet for the production of low-fat meat.

کلیدواژه‌ها [English]

  • Diet energy
  • Gene expression
  • Gene ontology
  • lipogenesis pathway
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