اثرات سم عقرب آندروکتونوس کراسیکودا بر آزاد سازی سایتوکین های پیش التهابی و ضد التهابی در موش صحرایی

نوع مقاله : مقاله کامل

نویسندگان

1 گروه جانوران سمی و تولید پادزهر، موسسه تحقیقات واکسن و سرم سازی رازی شعبه جنوب غرب کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران

2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

ترشح و فعال سازی واسطه های پیش التهابی از مهمترین عواملی است که در پاتوژنز سم عقرب نقش دارند. هدف از این مطالعه مقایسه میزان سایتوکین پیش التهابی(IL-6) و سایتوکین ضد التهابی(IL-10) در گروه موش های رت پس از تزریق زیر جلدی سم عقرب Androctonus crassicauda با گروه کنترل بوده است. برای انجام این مطالعه پس از تعیین LD50 سم عقرب آندرکتونوس کراسیکودا، به میزان 5/0 میلی گرم زیر جلدی به گروه دریافت کننده سم تزریق گردید. علاوه بر آن به تعداد 12 موش صحرایی به میزان 5/0 میلی لیتر سرم فیزیولوژی)هم حجم سم عقرب( به عنوان گروه کنترل به شکل زیر جلدی تزریق گردید. در زمان های4 24٬ و 72 ساعت بعد از تزریق سم، نمونه خون هپارینه از قلب حیوانات جمع آوری گردید. به منظور تعیین سطح پلاسمایی سایتوکین های( IL-6 و IL-10 ) از کیت تشخیصی الایزا شرکت ZellBio استفاده گردید.
میزان اینترلوکین 10 چهار ساعت بعد از تزریق سم از لحاظ آماری افزایش معنی داری نسبت به گروه کنترل نشان داد ولی مقدار اینترلوکین6 نسبت به گروه کنترل کاهش پیدا کرده بود. از طرفی میزان اینترلوکین 10 و اینترلوکین 6 ، بیست چهار ساعت بعد از تزریق سم عقرب نسبت به گروه کنترل تغییر معناداری نشان نداد و به حالت پایدار رسیدند. و در نهایت نتیچه گیری شد که علائم بالینی ناشی از عقرب زدگی به علت تعادل بین سایتوکین های پیش التهابی و ضد التهابی متغیر می باشد و این تعادل بین سایتوکین ها نقش محوری در بروز علائم بالینی در افراد عقرب زده دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Androctonus crassicauda scorpion venom on the release of proinflammatory and anti-inflammatory cytokines in rats

نویسندگان [English]

  • M Nemati 1
  • N Mohammadpour 1
  • A Zare Mirakabadi 1
  • F Salabi 1
  • M Khosravi 2
1 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran.
2 Department of Pathobiology, faculty of veterinary medicine, Shahid Chamran university of Ahvaz, Ahvaz,Iran
چکیده [English]

This study was performed to compare the levels of proinflammatory cytokine (IL-6) and anti-inflammatory cytokine (IL-10) in rats after subcutaneous injection of Androctonus crassicauda scorpion venom with control group that received only physiological serum subcutaneously. 0.5 mg of Androctonus crassicauda scorpion venom with LD50 400mg / kg was injected subcutaneously into 12 rats in the venom group. In addition, 0.5 ml of physiological serum (equal volume of scorpion venom) was injected subcutaneously into 12 rats in the control group. At 0,4,24 and 72 hours after injection of the venom (28), heparinized blood samples were collected via the heart of animals and the plasma levels of cytokines (IL-6 and IL-10) were determined using the ZellBio ELISA diagnostic kit.
Four hours after injection of the venom, the level of interleukin 10 was statistically significantly increased compared to the control group, but the level of interleukin 6 was decreased compared to the control group. 24 hours after injection of the venom, the levels of interleukin 10 and interleukin 6 did not show a significant change compared to the control group and reached a stable state.
Finally, it was concluded that due to the balance between proinflammatory and anti-inflammatory cytokines (IL-6 and IL-10), the clinical symptoms of scorpion stings are variable. And the balance between cytokines plays a pivotal role in the development of clinical symptoms in scorpion stings.
 

کلیدواژه‌ها [English]

  • Androctonus crassicauda Scorpion
  • Pro-inflammatory cytokines
  • Anti-inflammatory cytokines
  • Rat
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