بررسی شیوع آلودگی به تک‌یاخته بلاستوسیستیس در انسان و پرندگان اهلی

عبداله یی, سمانه; دلیمی اصل, عبدالحسین; پیرستانی, مجید

doi:10.22092/vj.2022.358234.1963

بررسی شیوع آلودگی به تک‌یاخته بلاستوسیستیس در انسان و پرندگان اهلی

نوع مقاله : مقاله کامل

نویسندگان

¹ گروه انگل شناسی و حشره شناسی پزشکی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس

² گروه انگل شناسی وحشره شناسی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس

³ گروه انگل شناسی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس، تهران

10.22092/vj.2022.358234.1963

چکیده

بلاستوسیستیس انگل روده‌ای بی‌هوازی و شایع در بین انسان‌ها و حیوانات مختلف در سراسر جهان است. برای تشخیص بلاستوسیستیس از روش‌های مختلفی مانند روش مستقیم، رنگ‌آمیزی و مولکولی می‌توان استفاده کرد. هدف از این مطالعه، بررسی آلودگی بلاستوسیستیس در انسان و پرندگان اهلی در شهرستان تفرش و مقایسه سه روش در تشخیص بلاستوسیستیس بوده است. مطالعه حاضر، به صورت مشاهده‌ای-توصیفی در خلال سال‌های 1399-۱400 در شهرستان تفرش طراحی و اجرا گردید. بدین‌منظور از کبوتران (300 نمونه)، مرغ و خروس‌ها (50 نمونه) و از صاحبان آنها (100 نمونه) نمونه مدفوع جمع‌آوری شد. سپس مدفوع ابتدا با روش‌ میکروسکوپی (آزمایش مستقیم و رنگ‌آمیزی با تری‌کروم) از لحاظ ابتلا به بلاستوسیستیس مورد مطالعه قرار گرفت سپس نمونه‌های مثبت از لحاظ مولکولی با روش PCRو از طریق تکثیر ژن 18S rRNA تشخیص داده شد.
طبق نتایج حاصله، در آزمایش مستقیم 39 نمونه کبوتر (13%)، 13 نمونه مرغ و خروس ( 26%) و 18 نمونه مدفوع انسانی ( 18%) و در آزمایش رنگ‌آمیزی با تری‌کروم، 45 نمونه کبوتر (15%)، 12 نمونه مرغ و خروس (24%) و 15 نمونه مدفوع انسانی ( 15%) از نظر وجود انگل بلاستوسیستیس مثبت تشخیص داده شد. در آزمایش PCR از بین نمونه‌های مدفوع مثبت کبوتران، یک نمونه (5/2%) و از بین نمونه‌های مدفوع مثبت مرغ و خروس، فقط 5 نمونه (46/38٪) و از نمونه‌های مدفوع مثبت انسانی، ۴ نمونه (22/22%) از نظر وجود انگل بلاستوسیستیس، مثبت تشخیص داده شد. در مطالعه حاضر فقط تعداد محدودی از نمونه‌های مثبت مرتبط با روش‌های مستقیم و رنگ‌آمیزی شده با تری‌کروم در آزمایش PCR مثبت شدند، این نشان می‌دهد که روش PCR با استفاده از یک جفت پرایمر به تنهایی روشی مناسب برای تعیین میزان آلودگی نیست. لذا پیشنهاد می‌شود برای تشخیص دقیق بلاستوسیستیس از چندین روش متفاوت استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

Prevalence of Blastocystis protozoan infection in humans and domestic birds

نویسندگان [English]

Samaneh Abdolahi ¹
Abdolhossein Dalimi ²
Majid Pirestani ³

¹ Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University

² Parasitology Department, Faculty of Medical Sciences, Tarbiat Modares University

³ Parasitology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran

چکیده [English]

Blastocystis is an anaerobic intestinal parasite that is prevalent among humans and various animals throughout the world. Different methods such as direct and staining stool smear examination as well as molecular methods can be used to diagnose Blastocystis.
The main objective of the present work was to study Blastocystis infection in humans and domestic birds in Tafresh city and to compare three methods in diagnosing Blastocystis. The present study was designed and conducted as a cross-sectional descriptive study during the years 1399-1400 in Tafresh city. For this purpose, fecal samples were collected from pigeons (300 samples), hens and roosters (50 samples) and from their owners (100 samples). Stools were then examined microscopically (direct examination and trichrome staining) for Blastocystis detection. The positive samples were tested molecularly by PCR (18S rRNA amplification).
In direct stool examination, 39 samples of pigeon feces (13%) and 13 samples of hen and rooster feces (26%) and 18 samples of human feces (18%) and in trichrome staining examination, 45 samples of pigeon feces (15%), 12 samples of hen and rooster feces (24%) and 15 samples of human feces (15%) were found to be positive for Blastocystis. In PCR test, only one sample (2.5%) of pigeon positive samples and 5 samples (38.46%) of hen and rooster positive samples and 4 samples (22.22%) of human positive samples were found to be positive. In the present study, only a limited number of positive samples related to direct stool examination and stained with trichrome were found to be positive in PCR experiment. This indicates that PCR method alone is not an appropriate method to determine the infection rate of Blastocystis. Therefore, it is suggested to use several different techniques for accurate diagnosis of Blastocysts.

کلیدواژه‌ها [English]

Blastocystis
Human
Bird
Infection rate

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