مقایسه قدرت تولید توکسین توسط تیپ‌های مختلف کلستریدیوم پرفرنجنس درجدایه‌های گوسفند و بز با روش الیزا

نوع مقاله : مقاله کامل

نویسندگان

1 گروه میکروبیولوژی، واحد کرمان، دانشگاه آزاد اسلامی، کرمان، ایران

2 بخش تحقیقات و فناوری، شعبه کرمان، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمان، ایران

چکیده

کلستریدیوم پرفرنجنس جزو کلستریدیوم‌هایی قرار می‌گیرد که بیماری‌زایی آن‌ها به دلیل تولید توکسین و زهرابه کشنده‌ای است که در داخل بدن ترشح می‌کنند و ضررهای اقتصادی زیادی را به صنعت دام وارد می‌کنند. کلستریدیوم پرفرنجنس چهار نوع توکسین اصلی آلفا، بتا، اپسیلون و یوتا تولید می‌کند. در مطالعه حاضر تعداد 84 جدایه از تیپ‌های مختلف کلستریدیوم پرفرنجنس که از گوسفند و بز با روش‌های بیوشیمیایی و میکروبی جداسازی و با روش PCR تعیین تیپ شده بودند جهت ارزیابی قدرت تولید توکسین‌های آلفا، بتا و اپسیلون در شرایط برون‌تنی (In Vitro) با روش الیزا مورد بررسی قرار گرفتند. براساس نتایج بدست آمده از تعداد 84 جدایه، 64 جدایه حضور حداقل یک توکسین را در آزمایش الیزا نشان دادند. همچنین در 31 جدایه حضور فقط توکسین آلفا، شش جدایه فقط توکسین اپسیلون، 17 جدایه توکسین آلفا و اپسیلون، شش جدایه توکسین آلفا و بتا و چهار جدایه هر سه توکسین آلفا، اپسیلون و بتا تایید شد و در 20 جدایه حضور هیچ کدام از توکسین‌ها شناسایی نشد. نتایج حاصل با نتایج تعیین تیپ به روش PCR که روی جدایه‌ها انجام شده بود نیز مقایسه شد. با بررسی فراوانی و توزیع توکسین‌های مختلف در تیپ‌های مختلف مشخص شد که در جدایه‌های تیپ C بیشترین موارد حضور توکسین آلفا و بتا و جدایه‌های تیپ B بیشترین موارد حضور توکسین اپسیلون را نشان دادند که مشخص کرد بیماری‌زایی مرتبط با این تیپ‌ها در جدایه‌های مورد مطالعه وجود دارد.  

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of toxin production power in different types of Clostridium perfringens among sheep and goats isolates by ELISA

نویسندگان [English]

  • Maryam Amini 1
  • Mehrdad Shamsaddini Bafti 2
  • BabaK Kheirkhah 1
  • Farokha Rokhbakhsh-Zamin 1
1 Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
2 Department of Research and Technology, Kerman Branch, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
چکیده [English]

Clostridium perfringens (C. perfringens) is one of the most common pathogens that produce an array of toxins that are secreted in the body which cause economic losses in the livestock industry. C. perfringens has four major toxins: alpha, beta, epsilon, and iota. A total of 84 isolates of C. perfringens from various types that originated from sheep and goats' isolates that were identified as C. perfringens by microbiological, biochemical, and toxin typed by PCR method were evaluated for production of alpha, beta, and epsilon toxin by ELISA method. Results showed that out of 84, 64 isolates showed the presence of at least one toxin in ELISA test, 31, and six isolates were positive for only alpha and only epsilon toxin, respectively, 17, six isolates show the presence of both alpha and epsilon and both alpha and beta toxins, respectively furthermore four isolates positive for the presence of all three toxins, meanwhile, 20 C. perfringens isolates didn’t show any of the toxins. The results were compared with the results of toxinotyping by PCR method. The frequency and distribution of toxins in different strains were evaluated, so type C isolates showed the most positive cases of alpha and beta toxin and type B isolates showed the most positive cases of epsilon toxin, which could be related to the pathogenicity of these types in the isolates studied

کلیدواژه‌ها [English]

  • Clostridium perfringens
  • Toxinotyping
  • Alpha
  • Beta
  • Epsilon

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سابقه مقاله

  • تاریخ دریافت: 14 شهریور 1400
  • تاریخ بازنگری: 01 آبان 1400
  • تاریخ پذیرش: 19 آبان 1400

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