اپیدمیولوژی و تشخیص ملکولی بابزیا بایجمینا و بابزیا بوویس در گاوهای استان لرستان

نوع مقاله : مقاله کامل

نویسندگان

1 بخش تحقیقات بیماریهای انگلی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

2 شعبه شیراز، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، شیراز، ایران

چکیده

بابزیوزیس یک بیماری منتقله از کنه است که توسط تک‌یاخته‌های خونی جنس بابزیا ایجاد می‌شود. هدف از این مطالعه شناسایی گونه‌های بابزیا بایجمینا و بابزیا بوویس در گاوهای استان لرستان بود. در مجموع تعداد 258 نمونه خون از طریق ورید وداج گاوهای به‌ظاهر سالم به‌طور تصادفی اخذ شد. در ابتدا DNA استخراجی از نمونه‌های خونی با جفت آغازگری که قطعه حدود 400 جفت بازی از ژن 18S rRNA جنس بابزیا را تکثیر می‌کرد، تکثیر شد. تمامی نمونه‌های مثبت گاوی با nested-PCR semi اختصاصی از نظر وجود بابزیا بایجمینا و بابزیا بوویس بررسی شدند و آلودگی نمونه‌های گاوی از نظر بابزیا بایجمینا و بابزیا بوویس به ترتیب 8/17  و 4/19 درصد تشخیص داده شد. آزمون مربع کای جهت مقایسه میزان شیوع نسبت به اقلیم، ارتفاع از سطح دریا، طول جغرافیایی، عرض جغرافیایی، نوع دامداری از نظر مدیریت، سطح بهداشتی دامداری، حضور کنه روی بدن دام، سم‌پاشی در فصول تکثیر کنه،  فاصله با دامداری‌های دیگر، تماس با نشخوارکنندگان وحشی، تراکم دام در دامداری، نژاد دام، سن دام و جنس دام انجام شد. در مقایسه فراوانی گونه بابزیا بایجمینا تنها در مورد تراکم دام در دامداری اختلاف معنی‌داری مشاهده شد (05/0≥P). در مدل لجستیک چند متغیره نوع دامداری و تراکم دام در دام‌داری به‌عنوان مهم‌ترین عوامل خطر در ابتلا به بابزیا بوویس معرفی شدند (01/0≥P). نتایج این مطالعه می‌تواند در برنامه‌ریزی راهبردی پیشگیری و کنترل بابزیوزیس گاوی در غرب ایران مورد استفاده قرار گیرد

کلیدواژه‌ها


عنوان مقاله [English]

Molecular detection and prevalence of B. bigemina and B. bovis in cattle in Lorestan province, Iran

نویسندگان [English]

  • V Noaman 1
  • M Namavari 2
1 Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
2 Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural ‎Research Education and Extension Organization (AREEO), Shiraz, Iran.‎
چکیده [English]

Babesiosis is a tick-borne disease caused by protozoa of the genus Babesia. This study aimed to determine the B. bigemina and B. bovis among cattle of Lorestan Province. A total of 258 blood samples were collected via the jugular vein from healthy cattle, randomly. The extracted DNA from blood cells was amplified by Babesia-all primers, which amplify an approximately 400bp DNA fragment from the region of the 18S rRNA gene from various members of the genus Babesia. All cattle positive samples were further analysed for the presence of B. bigemina and B. bovis by specific semi-nested PCR. B. bigemina and B. bovis were identified by specific semi-nested PCR in 17.8% and 19.4% of cattle blood samples, respectively. Chi-square tests were used to compare molecular prevalence values relative to climate, altitude, longitude, latitude,  farm type, hygiene, vectors, use of acaricide, distance from other farms, contact with wild ruminants, farm density, race, age, and sex were recorded for each animal. Among these factors, a significant association was only found between the prev-alence of B. bigemina infection and farm density (P< 0.05). Multivariable logistic regression analysis revealed that farm type and farm density were significant risk factors for B. bovis infection in cattle of Lorestan province (P< 0.01). The results of this study can be used in strategic planning for the prevention and control of bovine babesiosis in dairy cattle in the west of Iran.

کلیدواژه‌ها [English]

  • Iran
  • Lorestan province
  • B. bigemina
  • B. bovis
  • Cattle
  • molecular detection
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