ارزیابی سیستم ایمنی موش‌های BALB/c در برابر فرم لیوفلیزه نئوسپورا کانینوم

نوع مقاله : مقاله کامل

نویسندگان

1 واحد کرج، دانشگاه آزاد اسلامی ، کرج ، ایران

2 گروه انگل شناسی ، واحدکرج، دانشگاه آزاداسلامی، کرج، ایران

3 موسسه رازی شعبه شیراز. شیراز. ایران

چکیده

در این مطالعه، هدف بررسی اثر لیوفلیزاسیون روی سوش تخفیف حدت‌یافته در مقایسه با سوش حاد در ایجاد پاسخ ایمنی در موش BALB/c می‌‌باشد. بدین منظور 40 سر موش BALB/c در پنج گروه هشت‌تایی تقسیم شدند. گروه اول سوش حاد NC1، گروه دوم سوش حاد NC1به صورت لیوفلیزه، گروه سوم سوش تخفیف حدت یافته NCR، گروه چهارم سوش تخفیف حدت NCR به صورت لیوفلیزه و گروه پنجم گروه کنترل. برای انجام آزمون‌های الایزا و اینترفرون گاما سه هفته پس از تزریق، خون‌گیری صورت گرفت. نتایج نشان دادند که سیستم ایمنی هومورال به خوبی عمل کرده است و در هر چهار گروه ایمن شده پاسخ ایمنی هومورال نسبت به گروه کنترل تفاوت معنی‌دار دارد (05/0>P value). این مطالعه نشان داد که سوش تخفیف حدت‌یافته به خوبی قادر به ایجاد پاسخ ایمنی هومورال و ایمنی سلولی می‌باشد و می‌تواند به عنوان کاندیدی مناسب جهت مطالعات بیشتر برای تولید واکسن مطرح گردد. 

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of immune response of BALB/c mice against lyophilized form of Neospora caninum

نویسندگان [English]

  • N tavassoli 1
  • M kefayat 2
  • M.M Namavari 3
1 Karaj branch,Islamic azad university,karaj, iran
2 department of Parasitology, karaj branch, Islamic azad university,karaj, iran
3 Razi Vaccine and Serum Research Institute Shiraz, Shiraz,, Iran
چکیده [English]

The aim of this study was to investigate the effect of lyophilization on which strain in comparison with acute strain in the development of immune response in BALB/c mice. To this end, 40 BALB/c mice were divided into 5 groups each of 8 heads. The first group: NC1 (acute strain), the second group: lyophilized NC1 (acute strain), the third group: NCR (attenuated strain), the fourth group: lyophilized NCR (attenuated strain), and the fifth group: control. Three weeks after induction of vaccines, blood sampling was performed for ELISA and interferon-gamma tests. The results showed that the hemorrhagic immune system performed well (P <0.05, OR (CI95%)), and all four immunosuppressed groups had a significant difference in humoral immune response compared to the control group. An important result was the interferon test, which revealed that the lyophilized form in the acute strain had no significant difference in the level of interferon gamma compared to the control group, while lyophilized attenuated strain also had a significant difference between the levels of IFN-γ compared to the control (P <0.05, OR (CI95%)). This study showed that the lyophilized attenuated strain N.caninum is also able to produce a humoral and cell mediated immune and can be considered as an appropriate candidate for further studies on vaccine production.

کلیدواژه‌ها [English]

  • Neospora caninum
  • lyophilization
  • BALB/c Mice
  • Immunity
  • vaccine
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