اعتبارسنجی نوارهای ادراری انسانی به لحاظ پارامترهای مختلف بیوشیمیایی در سگ

نوع مقاله : مقاله کامل

نویسندگان

1 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شهید باهنر کرمان. کرمان. ایران

2 گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه شهید باهنر کرمان، کرمان، ایران

3 گروه علوم پایه، دانشکدۀ دامپزشکی، دانشگاه شهید باهنر کرمان، کرمان، ایران

4 گروه علوم درمانگاهی، دانشکدۀ دامپزشکی، دانشگاه شهید باهنر کرمان، کرمان، ایران

چکیده

تجزیه و تحلیل ادرار اطلاعات ارزشمندی در مورد شرایط فیزیولوژیکی و آسیب‌شناسی بیماران ارائه می‌دهد. هدف از این مطالعه، تعیین میزان دقت نوار ادراری انسان برای ارزیابی پارامترهای مختلف بیوشیمیایی مانند pH، وزن مخصوص، پروتئین، گلوکز، بیلی‌روبین و لکوسیت در نمونه‌های ادرار سگ بود. بدین منظور، نمونه ادرار 60 سگ جمع‌آوری شد و نتایج اندازه‌گیری پارامترهای ادراری با استفاده از هر دو نوار ادراری تجاری مختلف (کیمیا و MN) و آزمایشات استاندارد مورد تجزیه و تحلیل قرار گرفت. ضریب همبستگی بین پروتئین اندازه‌گیری شده ادرار با دو نوار ادراری و روش استاندارد متوسط بود (68/0=r). برای گلوکز، ضریب همبستگی بین روش مرجع و نوار ادرار خوب بود (78/0=r) در مورد پروتئین و گلوکز، هر دو نوار حساسیت و ارزش پیش‌بینی منفی (NPV) عالی را نشان دادند. همبستگی بین pH ادرار سنجش شده با pH متر و نوارها بسیار عالی بود (91/0=r). اگرچه همبستگی بین وزن مخصوص ادرار با استفاده از رفراکتومتر و نوارها معنی‌دار بود (38/0= r)، ضریب همبستگی ضعیف بود. به نظر می‌رسید که استفاده از نوار ادراری انسان برای ارزیابی وزن مخصوص روش مناسبی نبوده و رفراکتومتر ترجیح داده می‌شود. پیشنهاد می‌شود که می‌توان از نوار ادراری انسان برای تعیین پروتئین، گلوکز و pH در ادرار سگ استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Validation of human urinary dipsticks for different biochemical parameters in dog

نویسندگان [English]

  • N Eskandarzade 1
  • M Zamani-Ahmadmahmudi 2
  • F Hekmatynia 3
  • M Rezaei 4
  • M Imani 4
1 Basic sciences,, School of Veterinary Medicine. Shahid Bahonar University of Kerman. Kerman. Iran
2 Department of Clinical Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
3 Basic sciences,, School of Veterinary Medicine. Shahid Bahonar University of Kerman. Kerman. Iran
4 Department of Clinical Science, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده [English]

Urinalysis provides the valuable information about physiological and pathological conditions of the patients. The aim of this study was to determine accuracy of human urinary dipsticks for assessment of different biochemical parameters such as pH, specific gravity, protein, glucose, bilirubin and leukocyte in canine urine samples. For this purpose, urine samples of 60 dogs were collected and paired measurements of the urinary parameters with both different commercial dipsticks (Kimia and MN) and standard tests were analyzed. The correlation coefficient was fair between the assayed urine protein with two urine strips and standard method (r=0.68). For glucose, correlation coefficient was good between reference method and the urine strips (r=0.78). In the case of protein and glucose, both strips showed excellent sensitivity and negative predictive value (NPV). Correlation coefficient was good between reference method and the urine strips (r=0.91). Although correlation was significant between urine specific gravity assayed using refractometer and strips (r=0.38), correlation coefficient was poor. It seemed that employing human urine strips was not suitable method for evaluating specific gravity and refractometer was preferred. In conclusion, it is suggested that human urine strips can be used to determine protein, glucose and pH in urine of dog.  

کلیدواژه‌ها [English]

  • Urinalysis
  • Validation
  • Urinary dipsticks
  • Dog

مراجع

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سابقه مقاله

  • تاریخ دریافت: 09 تیر 1400
  • تاریخ بازنگری: 07 شهریور 1400
  • تاریخ پذیرش: 08 شهریور 1400

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