مقایسه روش های میکروسکوپیک و ملکولی در تشخیص آناپلاسما مارجیناله و تعیین عوامل خطر مرتبط در گاوهای استان سمنان

نوع مقاله : مقاله کامل

نویسندگان

1 بخش تحقیقات بیماریهای انگلی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

2 گروه پاتوبیولوژی دانشکده دامپزشکی دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران

چکیده

هدف از این مطالعه تعیین شیوع آناپلاسما مارجیناله در جمعیت گاوهای سنتی و نیمه‌صنعتی استان سمنان بود. در مجموع تعداد 208 نمونه خون از طریق رگ گوش و رگ وداج گاوهای به‌ظاهر سالم به‌طور تصادفی به ترتیب برای آزمون میکروسکوپی و ملکولی اخذ شد. DNA استخراجی از نمونه‌های خونی با جفت آغازگری که قطعه حدود 866 جفت بازی از ژن msp4 آناپلاسما مارجیناله را تکثیر می‌کرد، تکثیر شد و 2/45 درصد از نمونه‌ها از نظر آناپلاسما مارجیناله مثبت تشخیص داده شدند. آزمون مربع کای جهت مقایسه میزان شیوع نسبت به اقلیم، ارتفاع از سطح دریا، طول جغرافیایی، عرض جغرافیایی، فصل، نوع دامداری از نظر مدیریت، سطح بهداشت دامداری، ناقلین موجود در دامداری، تراکم گله سن، جنس، تولید شیر انجام شد. در آزمون لجستیک چند متغیره عرض جغرافیایی، نوع دامداری از نظر مدیریت، ناقلین موجود در دامداری، سن و تولید شیر عوامل خطر مهم برای ابتلا به آناپلاسما مارجیناله معرفی شدند (0.05>P). در مقایسه با آزمون ملکولی، حساسیت و ویژگی آزمون میکروسکوپی به ترتیب 9/31 و 4/54 درصد تعیین گردید. محاسبه ضریب کاپا بین آزمون ملکولی و میکروسکوپی آناپلاسما مارجیناله (50 فیلد) نشان‌دهنده سطح ناچیز توافق دو آزمون بود (0.13= Kappa). این مطالعه اولین مطالعه تشخیص ملکولی آناپلاسما مارجیناله در گاوهای استان سمنان است. تحقیقات دیگری در جهت شناسایی ناقلین، اثر متقابل میزبان- ناقل و شناسایی واریته های ژنتیکی که ممکن است حضور و گسترش گونه‌های آناپلاسما را در گاوهای استان سمنان تحت تأثیر قرار دهند موردنیاز است.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of microscopic and molecular methods in the diagnosis of Anaplasma marginale and determination of related risk factors in cattle in Semnan province

نویسندگان [English]

  • V Noaman 1
  • P Adami-Dehkordi 2
  • S. Sh. R Shojaei 2
1 Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
2 Department of pathobiology, Faculty of veterinary medicine, Karaj branch, Islamic Azad University, Karaj, Iran
چکیده [English]

This study aimed to determine the molecular prevalence of Anaplasma marginale among cattle of Semnan province. A total of 208 blood samples were randomly collected via the ear vein and jugular vein from healthy cattle for microscopy and molecular examination, respectively. The extracted DNA from blood cells was amplified by a pair of primer, which amplify an approximately 866bp DNA fragment from the region of the msp4 gene of  A. marginale and 45.2% of cattle blood samples were identified positive for A. marginale. Chi-square tests were used to compare molecular prevalence values relative to climate, altitude, longitude, latitude, season, farm-type, hygiene of the farm, vectors, farm density, age, sex, and milk yield. The significant major risk factors of A. marginale in cattle were identified as latitude, farm-type, vectors, age, and milk yield by the multivariable logistic regression analysis (P< 0.05). The examination of 50 microscopic fields showed 31.9% sensitivity and 54.4% specificity compared to molecular examination. The Kappa coefficient between molecular and microscopy (50 fields) techniques indicated a slight level of agreement (Kappa= 0.13). This study is the first molecular detection of A. marginale from cattle in Semnan province, Iran. Further researches are needed to determine the vectors, vector-host interactions, and genotypic variants that may affect the presence and distribution of Anaplasma species in Semnan province.

کلیدواژه‌ها [English]

  • Anaplasma marginale
  • Cattle
  • Microscopic and molecular detection
  • Semnan province
  • Iran

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  • تاریخ دریافت: 29 شهریور 1399
  • تاریخ بازنگری: 13 مهر 1399
  • تاریخ پذیرش: 10 آبان 1399

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