تاثیر استفاده از دانه کتان بعنوان منبع امگا 3 برمیزان تولید شیر و بیان ژن های درگیر در رشد و نمو بافت پستان در بزهای سانن

نوع مقاله : مقاله کامل

نویسندگان

1 موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد ورامین پیشوا، تهران، ایران

3 گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد ورامین- پیشوا، تهران، ایران

چکیده

به منظور بررسی اثر مصرف دانه کتان به عنوان منبع اسید چرب امگا-3 بر بیان ژن‌های مؤثر در رشد و نمو بافت پستان در بزهای سانن آبستن شکم اول، تعداد 30 رأس گیسه (بز ماده جوان) که در نیمه دوم اولین آبستنی خود بودند، انتخاب و پس از وزن‌کشی به سه گروه 10 رأسی تقسیم شدند. یک گروه به عنوان شاهد منفی، جیره‌ای فاقد هرگونه منبع چربی دریافت نمود، گروه دوم دریافت‌کننده جیره حاوی چربی اشباع پالم (شاهد مثبت) و گروه سوم دریافت‌کننده دانة بزرک (کتان) اکسترود شده به عنوان منبع امگا-3 بودند. جیره‌های هر سه گروه بر اساس احتیاجات بز شیری NRC 2007 تنظیم شدند. گروه‌های آزمایشی از 2 ماه پایانی آبستنی تا 12 هفته پس از زایش تیمارهای تغذیه‌ای را دریافت نمودند. طی دوره آزمایش، تولید شیر برای هر حیوان به‌طور هفتگی ثبت شد و نمونه‌گیری از بافت پستان در سه نوبت، 24 الی 36 ساعت بعد از زایش، دو و چهار ماه پس از زایش به منظور بررسی بیان ژن‌های IGF-1،IGFBP3 و IGFBP5 انجام شد. نتایج حاصل از این تحقیق نشان داد که بین تیمارها از نظر بیان ژن‌های مورد مطالعه تفاوت معنی داری وجود ندارد. همچنین برهمکنش بین تیمار و زمان نیز برای هیچکدام از ژن‌های فوق معنی‌دار نبود و تنها اثر زمان برای ژن IGFBP-3 معنی دار شد (05/0>P).

کلیدواژه‌ها

عنوان مقاله [English]

Effect of using flaxseed as omega-3 source on mammary gland growth and development gene expression in Saanen goat at their first pregnancy and lactation

نویسندگان [English]

  • H. R. Seyedabadi, 1
  • Gh. Asgari Jafarabadi 2
  • A. R Mahmoodi 3
  • H. Javaheri Barforosh 1
1 Animal Science Research Institute of IRAN (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
2 Department of Animal Science, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Tehran, Iran
3 Department of Animal Science, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

In order to investigate the effect of flaxseed consumption as a source of omega-3 fatty acids on the expression of genes effective in mammary gland tissue development in primiparous Sanan goats, 30 does (young female goats) in the second half of their first pregnancy were selected and divided into three groups of 10 based on their average initial live weight . One group received a diet without any fat source (negative control), the second group received a diet containing palm saturated fat (positive control) and the third group received extruded flaxseed (flax) as a source of omega-3. The diets of all three groups were adjusted according to the requirements of NRC 2007 dairy goats. Experimental groups received nutritional treatments from the last 2 months of pregnancy up to  4 months after parturition. During the experimental period, milk production was recorded weekly for each animal and mammary gland tissue sampling was performed three times, 24 to 36 hours after parturition, two and four months after parturition were performed to investigation the expression of IGF- 1, IGFBP3 and IGFBP5 genes. The results of this study showed that there was no significant difference among the treatments in terms of expression of the studied genes. Also, the interaction between treatment and time was not significant for any of the above genes and only the effect of time was significant for IGFBP-3 gene (P <0.05).

کلیدواژه‌ها [English]

  • Dairy goat
  • omega-3
  • milk production
  • breast tissue
  • gene expression

مراجع

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  • تاریخ دریافت: 27 مرداد 1399
  • تاریخ بازنگری: 19 مهر 1399
  • تاریخ پذیرش: 20 مهر 1399

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