بررسی فعالیت آنتاگونیستی لاکتوباسیل های جدا شده از شیر و ماست گاومیش علیه بعضی از پاتوژن های غذایی در استان گلستان

نوع مقاله : مقاله کامل

نویسندگان

1 گروه میکروبیولوژی، دانشکدة علوم زیستی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

2 گروه میکروبیولوژی، واحد آزادشهر، دانشگاه آزاد اسلامی، آزادشهر، ایران

چکیده

لاکتیک اسید باکتری‌ها که به مقدار زیاد در فرآورده‌های لبنی یافت می‌شوند، علیه بسیاری از میکروارگانیسم‌های پاتوژن فعالیت آنتاگونیستی دارند. در این مطالعه اثر مهاری سویه های لاکتوباسیلوس جدا شده از شیر و ماست گاومیش در شهرستان بندرگز واقع در استان گلستان بررسی شد. نمونه‌های شیر خام و ماست پس از کشت در محیط MRS تحت شرایط بی‌هوازی در دمای 37 درجه سانتی‌گراد به مدت 48 ساعت گرمخانه‌گذاری شدند. کلنی‌های مشکوک بر اساس رنگ‌آمیزی گرم و آزمایش‌های بیوشیمیایی افتراقی شناسایی شدند. سپس فعالیت ضدباکتریایی محلول رویی استخراج شده از سویه‌های لاکتوباسیلوس با استفاده از روش انتشار در آگار علیه 5 سویه  استاندارد شامل ATCC 12022 Shigella flexneri، ATCC 14028 Salmonella typhimurium ،ATCC 17802 Vibrio paraheamolyticus  Escherichia coli ATCC 11303 و Staphylococcus aureus ATCC 29213 بررسی شد. در مجموع 10 سویه لاکتوباسیلوس شامل سویه‌های Lactobacillus plantarum ،‌Lactobacillus casei و Lactobacillus acidophilus جدا شدند. در بین جدایه‌ها L. plantarum A1  اثر مهاری خوبی در برابر S.aureus ، E. coli و S.flexneriاز خود نشان داد اما در برابر V. paraheamolyticus و S. typhimurium اثر مهارکنندگی نداشت. تنها جدایه‌ای که اثر مهاری روی V. paraheamolyticus داشت، جدایه L. acidophilus R1 بود. نتایج این مطالعه نشان داد که لاکتوباسیل‌های جدا شده از شیر و ماست گاومیش علیه بعضی از پاتوژن‌های غذایی اثر مهاری دارند و می‌توانند از گزینه‌های مطرح برای کنترل این عوامل در محصولات غذایی باشند. 

کلیدواژه‌ها

عنوان مقاله [English]

Antagonistic Activity of Lactobacilli Isolated from Buffalo Milk and Yogurt against Some Food Pathogens in Golestan province

نویسندگان [English]

  • R Ahmadi Liwani 1
  • A Ahani Azari 1
  • H Koohsari 2
1 Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
2 Department of Microbiology, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran
چکیده [English]

Lactic acid bacteria which are found in large amounts in dairy products, have antagonistic activity against many pathogenic microorganisms. In this study, the inhibitory effect of Lactobacillus strains isolated from buffalo milk and yogurt in Bandar Gaz city was investigated. Raw milk and yogurt samples were cultivated on MRS medium, incubating anaerobically at 37°C for 48 h. The suspected colonies were identified on the basis of Gram’s staining and biochemical tests. The antibacterial activity of the cell-free supernatant extracted from Lactobacillus strains was determined using the agar well diffusion method against standard strains including Shigella flexneri ATCC 12022 ، Salmonella typhimurium ATCC 14028 ، Vibrio paraheamolyticus ATCC 17802 ، Escherichia coli ATCC 11303 and Staphylococcus aureus ATCC 29213. A total 10 Lactobacillus strains were identified as L. plantarum, L. casei, L. acidophilus. The cell-free supernatant of L. plantarum A1 had a relatively strong inhibitory effect against S. aureus and a moderate inhibitory effect against E. coli and S. flexneri, while no inhibitory effect on V. paraheamolyticus and S. typhimurium growth. L. casei B1 showed a moderate inhibitory effect against S. flexneri and S. aureus. The supernatant of L. acidophilus R1 vs. S. aureus showed a moderate inhibitory effect and had an inhibitory effect on V. paraheamolyticus. The results of this study showed that Lactobacilli isolates had good inhibitory activity against some food pathogens, therefore further studies on their use as biopreservatives to prevent the survival and proliferation of microorganisms in food products are recommended. 

کلیدواژه‌ها [English]

  • Pathogens
  • Buffalo
  • Lactobacilli
  • Inhibitory

مراجع

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سابقه مقاله

  • تاریخ دریافت: 17 مرداد 1399
  • تاریخ بازنگری: 23 شهریور 1399
  • تاریخ پذیرش: 05 مهر 1399

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