تاثیر آنتی اکسیدان سیستیامین بر فراسنجه‌های سلولی و کیفیت اسپرم منجمد در قوچ

نوع مقاله : مقاله کامل

نویسندگان

1 گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

3 بخش تحقیق، پرورش و تولید حیوانات آزمایشگاهی، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

چکیده

هدف از اجرای این طرح بهبود بازده باروری اسپرم در گوسفند با افزودن آنتی‌اکسیدان‌ سیستیامین به محیط انجماد بوده است. در این آزمایش اسپرم از 5 رأس قوچ نژاد زندی جمع‌آوری به رقیق‌کننده‌های حاوی صفر، 1، 2، 4 و 8 میلی‌مولار سیستیامین اضافه و سپس منجمد شدند. در مرحله ارزیابی آزمایشگاهی کیفیت اسپرم، اسپرم‌های منجمد مورد ارزیابی آزمایشگاهی قرار گرفتند و فراسنجه‌های حرکتی، زنده‌مانی، سلامت غشا، مورفولوژی، سلامت آکروزوم، فعالیت میتوکندری و میزان لیپید پراکسیداسیون مورد ارزیابی قرار گرفتند. نتایج نشان داد که استفاده از 2 میلی مولار سیستیامین منجر به بهبود جنبایی کل، جنبایی پیشرونده، سلامت غشا، فعالیت میتوکندری، سلامت آکروزوم، زنده مانی و کاهش آپوپتوزیس و پراکسیداسیون لیپیدهای غشایی در اسپرم منجمد قوچ پس از یخگشایی شد. استفاده از تیمار 8 میلی مولار سیستیامین منجر به کاهش کیفیت اسپرم پس از یخگشایی شد و استفاده از آنتی اکسیدان سیستیامین تاثیری بر مورفولوژی اسپرم پس از فرایند انجماد-یخگشایی نداشت. در نتیجه استفاده از تیمار 2 میلی مولار آنتی اکسیدان سیستیامین می‌تواند راهکاری مناسب برای بهبود بازده اسپرم قوچ پس از فرایند انجماد-یخگشایی باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of cysteamine antioxidant on cellular parameters and frozen sperm quality in ram

نویسندگان [English]

  • Z. Abdollahi 1
  • R. Masoudi 2
  • N. Dadashpour Davachi 3
1 Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
3 Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

The aim of this study was improvement of ram frozen semen efficiency using addition of cysteamine antioxidant to freezing medium. In this experiment, semen samples were collected from five Zandi rams and add to the extender containing 0, 1, 2, 4 and 8 mM cysteamine. In sperm quality evaluation, sperm samples were evaluated and motion parameters, membrane integrity, morphology, acrosome integrity, mitochondria activity, viability and lipid peroxidation were assessed. The results showed using 2 mM cysteamine resulted in improving of sperm total and progressive motility, membrane integrity, mitochondria activity, acrosome integrity, and viability as well as reduction of apoptosis rate and lipid peroxidation. Using the treatment of 8 mM cysteamine caused to reduction of sperm quality after thawing and the antioxidant did not have any effect on sperm morphology. In conclusion, using 2 mM cysteamine in freezing extender could be a helpful strategy for improvement of ram sperm quality after cryopreservation.

کلیدواژه‌ها [English]

  • Sperm freezing
  • Sperm evaluation
  • Cysteamine
  • Ram
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