مطالعه چندشکلی در ژن دی آسیل گلیسرول آسیل ترانسفراز (DGAT1) شترهای تک‌کوهانه خوزستان

نوع مقاله : مقاله کامل

نویسندگان

1 گروه علوم دامی دانشگاه علوم کشاورزی و منابع طبیعی خوزستان

2 گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران

چکیده

ژن DGAT1 با کد‌کردن آنزیم دی آسیل گلیسرول آسیل ترانسفراز (DGAT1) نقش اصلی را در ساخت تری‌گلیسیرید و چربی شیر دارد. تحقیقات اخیر نشان داده که چندشکلی در ژن DGAT1 بر صفت تولید شیر موثر است. لذا هدف از این تحقیق بررسی چندشکلی اگزون 14 ژن DGAT1 در شترهای تک‌کوهانه خوزستان با استفاده از روش چندشکلی شکل فضایی رشته‌های منفرد (SSCP) بود. برای انجام این آزمایش از 80 نفر شتر تک‌کوهانه خونگیری به عمل آمد. پس از استخراج DNA‌، یک قطعه 310 جفت بازی از اگزون 14 ژن DGAT1 با استفاده از واکنش زنجیره پلی مراز (PCR) تکثیر گردید. در این مطالعه 6 الگوی باندی متفاوت به عنوان شش ژنوتیپ متفاوت شامل AA، AB، BB، CC، DD و EE به ترتیب با فراوانی‌های 45 ، 75/33 ، 5، 75/3 ، 5/2 و 10 درصد و پنج نوع آلل A، B، C، D و E به ترتیب با فراوانی‌های 619/0، 219/0، 038/0، 025/0، 1/0 شناسایی گردید. بعلاوه، آزمون کای‌مربع نشان داد که جمعیت در این جایگاه در تعادل هاردی-‏وینبرگ قرار ندارد. تعداد آلل موثر، شاخص شانون، هتروزیگوسیتی مورد‏انتظار و مشاهده شده برای این جایگاه به ترتیب 259/2، 07/1، 557/0 و 338/0 محاسبه گردید. این نتایج نشان دهنده‏ی وجود چندشکلی است اما نشان می‌داد که میزان هتروزیگوسیتی در جمعیت مورد مطالعه کم است. پیشنهاد می‌گردد با تدوین برنامه‌های اصلاح نژادی مناسب تنوع موجود در جمعیت شتر تک‌کوهانه خوزستان افزایش داده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Study of polymorphism in exon 14 of Diacylglycerol O-Acyltransferase 1 (DGAT1) Gene in Dromedary Camel of Khuzestan

نویسندگان [English]

  • E. Yavari 1
  • J. Fayazi 2
  • M Nazari 2
  • Kh. Mirzadeh 2
1 Animal genetic and Breeding, Faculty of Animal Science and Food Technology, Agricultural science and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran
2 Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran
چکیده [English]

The Diacylglycerol O-Acyltransferase 1 (DGAT1) gene plays a key role in the production of triglycerides and milk fat by encoding the enzyme DGAT1. Recent research has shown that polymorphisms in the DGAT1 gene affect milk production. Therefore, the aim of this study was to investigate polymorphism in exon 14 region of DGAT1 gene in Khuzestan dromedary camels (Camelus dromedarius) using PCR-SSCP method. To perform this experiment, blood samples were collected from 80 dromedary camels in Khuzestan province. After DNA extraction, a 310-bp fragment of exon 14 region of DGAT1 gene was amplified using polymerase chain reaction (PCR). Six different SSCP patterns, including six different genotypes of AA (45%), AB (33.75%), BB (5%), CC (3.75%) , DD (2.5%) and EE (10%) and five alleles including A (0.619), B (0.219), C (0.038), D (0.025) and E (0.1) were identified. Moreover, the chi-square (χ2) test showed that the population was not in Hardy-Weinberg equilibrium. The number of effective alleles, Shannon index, expected and observed heterozygosity for this site was calculated 2.259, 1.07, 0.557 and 0.338, respectively. The results indicated polymorphism and low heterozygosity in the population under study. It is suggested to increase the diversity in the dromedary camel population of Khuzestan by developing appropriate breeding programs.

کلیدواژه‌ها [English]

  • Polymorphism
  • DGAT1
  • SSCP
  • Dromedary Camel
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