اثر تغذیه‌ای پودر برگ گیاه مورد (Myrtus communis) بر فراسنجه‌های کمی و کیفی اسپرم و عملکرد آنتی‌اکسیدانی منی و خون در قوچ عربی

نوع مقاله : مقاله کامل

نویسندگان

1 گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان

2 گروه بهداشت و مواد غذایی، دانشکده دامپزشکی دانشگاه شهید چمران اهواز

چکیده

هدف از این تحقیق بررسی تأثیر پودر برگ گیاه مورد با خاصیت آنتی‌اکسیدانی بر پارامترهای کمی و کیفی اسپرم، ظرفیت آنتی اکسیدانی پلاسمای منی و فعالیت آنزیم سوپراکسید دیسموتاز خون قوچ عربی در فصل غیرتولید‌‌مثلی بود. تعداد ۱۲ رأس قوچ عربی بالغ در قالب 3 تیمار آزمایشی و 4 تکرار استفاده شد. تیمارها شامل سطوح پودر برگ گیاه مورد (۰، ۵/۱۲ و ۲۵ گرم در کیلوگرم ماده خشک جیره) بودند. پس از 42 روز از تغذیه دام‌ها، اسپرم‌‌گیری و خون‌‌‌‌گیری به مدت ۴ هفته متوالی انجام و پارامترهای کمی و کیفی اسپرم و توان آنتی‌اکسیدانی تام پلاسمای منی ارزیابی شدند. همچنین، غلظت تستوسترون سرم خون و فعالیت سوپراکسید دیسموتاز پلاسمای خون مورد سنجش قرار گرفتند. سطح ۲۵ گرم پودر برگ مورد بهترین تأثیر در بهبود شاخص‌های اسپرم و منی شامل جنبایی (88/73%)، زنده‌‌مانی (19/74%)، ناهنجاری (10%)، سلامت غشای پلاسمایی اسپرم (38/43%)، حجم منی (91ml/1)، غلظت اسپرم (ml/109 ×39/1)، ظرفیت آنتی اکسیدانی تام پلاسمای منی (μmol/litr 38/109) و فعالیت سوپراکسید دیسموتاز خون (۱۹U/ml/۵۰۰) نسبت به سطح 5/12 گرم پودر برگ مورد و گروه شاهد داشت (05/0>P). غلظت تستوسترون سرم خون تحت تأثیر تیمارها قرار نگرفت. همبستگی مثبت و معنی‌داری میان توان آنتی‌اکسیدانی تام پلاسمای منی و سوپراکسید دیسموتاز پلاسمای خون با فراسنجه‌های کمی و کیفی اسپرم یافت شد (05/0>p). بطور کلی استفاده از پودر برگ گیاه مورد در جیره موجب بهبود فراسنجه‌های اسپرم و آنتی اکسیدانی پلاسمای منی و خون در قوچ عربی شد. بهترین نتیجه با استفاده از سطح 25 گرم در کیلوگرم جیره از این گیاه بدست آمد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of dietary Myrtus communis leaf powder on Quantitative and Qualitative Characteristics of Sperm and Antioxidant Function of Semen and Blood in Arabi Ram

نویسندگان [English]

  • S. Tabatabaei Vakili 1
  • E. Mohaysenzadeh 1
  • T. Mohammadabadi 1
  • M. Zarei 2
1 Department of Animal Science, Agricultural and Natural Resources University of Khuzestan
2 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz
چکیده [English]

The aim of this study was to investigate the effect of different levels of Myrtle leaf powder on quantitative and qualitative parameters of sperm, antioxidant capacity of semen plasma, and blood superoxide dismutase activity (SOD) in Arabi ram in non-breeding season. 12 adult Arabic rams were used in 3 experimental treatments with 4 replicates per treatment. The treatments consisted of 3 levels of Myrtle leaf powder (0, 12.5 and 25 g/kg of DM). After 42 days feeding of experimental treatments, semen samples were collected and blood samples were obtained once a week for 4 consecutive weeks and the quantitative and qualitative parameters of sperm and total antioxidant capacity of semen were measured. Also, blood samples were subjected to measure testosterone level and SOD activity. 25 g/kg of myrtle powder had the best effect on improvement the sperm and semen characteristics include the motility (73.88%), viability (74.19%), morphological defects (10%), plasma membrane integrity (43.38%), semen volume (1.91 ml) and spermatozoa concentration (1.39 × 109/ml) as well as increased the seminal plasma total antioxidant capacity (TAC) (109.38 μmol/litr) and blood plasma SOD (500.19 U/ml) (p < 0.05). The blood sera testosterone concentration was not affected by treatments. A positive and significant correlation was found between the TAC and SOD with quantitative and qualitative parameters of sperm in Arabi ram (p < 0.05). In conclusion, the use of Myrtle leaf powder in diet improved reproductive performance, seminal plasma TAC and blood SOD activity in Arabi ram. The best result was obtained from 25 g/kg of this plant.

کلیدواژه‌ها [English]

  • Antioxidants
  • Free radicals
  • Myrtle
  • Ram
  • Sperm

مراجع

1. Adeel, M., A. Ijaz, M. Aleem, H. Rehman, M.S. Yousaf and M.A. Jabbar. 2009. Improvement of liquid and frozen-thawed semen quality of Nili-Ravi buffalo bulls (Bubalus bubalis) through supplementation of fat. Theriogenology 71(8): 1220-1225.
2. Agarwal, A., R.A. Saleh and M.A. Bedaiwy. 2003. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertility and Sterility 79(4): 829-843.
3. Ahmad, M.K., A.A. Mahdi, K.K. Shukla, N. Islam, S. Rajender, D. Madhukar, S.N. Shankhwar and S. Ahmad. 2010. Withania somnifera improves semen quality by regulating reproductive hormone levels and oxidative stress in seminal plasma of infertile males. Fertilility and Sterility 94(3): 989-996.
4. Aidi Wannes, W., B. Mhamdi, J. Sriti and B. Marzouk. 2010. Changes in essential oil composition of Tunisian Myrtus communis var. italica during its vegetative cycle. Journal of Essential Oil Research 22: 3-18.
5. Alvarez, J.G. and B.T. Storey. 1995. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Molecular Reproduction and Development 42(3): 334-346.
6. Amidi, F., A. Pazhohan, M.S. Nashtaei, M. Khodarahmian and S. Nekoonam. 2016. The role of antioxidants in sperm freezing: a review. Cell Tissue Bank 17(4): 745-756.
7. Amini Rad, O., M.A. Khalili and H.R. Soltani Gord Faramarzi. 2009. Influence of pomegranate juice on sperm parameters and fertility in mice. Medical Journal of Hormozgan University 13(3): 182-188.
8. Angrimani, D.S.R., M. Nichi, J.D.A. Losano, C.F. Lucio, G.A.L. Veiga, M.V.J. Franco and C.I. Vannucchi. 2017. Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs. Journal of Animal Science and Biotechnology 8(1): 18.
9. Bearden, H.J. and J.W. Fuquay. 1980. Applied Animal Reproduction. Reston Publishing Company, Inc. P: 53.
10. Benzie, I.F.F. and J.J. Strain. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.
11. Bidmeshkipour, A., A. Hosseinzadeh Colagar, M. Gholinezhad Chari and P. Biparva. 2010. Seminal plasma total antioxidant capacity and vitamin- C levels in asthenozoospermia: a case- control study. Tehran University Medical Journal 67(12): 835-842.
12. Cassano, T., L. Pace, G. Bedse, A. Michele Lavecchia, F. De Marco, S. Gaetani and G. Serviddio. 2016. Glutamate and mitochondria: two prominent players in the oxidative stress-induced neurodegeneration. Current Alzheimer Research 13(2): 185-197.
13. DaghighKia, H., F. Sadeghi Sadeghabad, H. Mohamadzadeh, H. Vaseghi Dodran and I. Ashrafi. 2017. The effect of Origanum Vulgare extract as natural antioxidant on quality cryopreserved ram sperm. Animal Science Research 26(4): 111-120.
14. Daghighkia, H., R. Shahbaz zadeh and I. Ashrafi. 2015. Antioxidant effect of Macrantha Satureja extraction on microscopic and biochemical parameters of bull sperm after freeze-thawing process. Animal Science Journal 108(28): 101-112.
15. De Lamirande, E. and C. Cagnon. 1993. Human sperm hyperactivation and capacitation as parts of an oxidative process. Free Radical Biology and Medicine 14(2): 157-166.
16. Dos Santos Hamilton, T. R., C.M. Mendes, L.S. de Castro, P.M. de Assis, A.F.P. Siqueira, J. de Carvalho Delgado and J.A. Visintin. 2016. Evaluation of lasting effects of heat stress on sperm profile and oxidative status of ram semen and epididymal sperm. Oxidative Medicine and Cellular Longevity 1-12.
17. Esmaeili Sabzevar, H., R. Rahbarian, M. Saleh Moghadam and S.D. Sadoughi. 2017. Effect of aqueous extract of mangrove leaves (Avicennia marina) on the antioxidant enzyme activities of the ovarian tissue in diabetic rats. Journal of Health Chimes 5(1): 32-41.
18. Evan, G. and W.M.C. Maxwell. 1987. Salamon’s Artificial Insemination of sheep and goat. Butter woth, Sydney, Australia.
19. Ezzatpour, M. 2003. Sheep and goat breeding in Iran. Moalef publication, Tehran, 181.
20. Faraji, Z., H. Nikzad, K. Parivar and M. Nikzad. 2013. The effect of aqueous extract of Salep Tubers on the structure of testis and sexual hormones in male mice. Journal of Jahrom University of Medical Science 11(1): 71-76.
21. Farhadi, R., H. Daghighkia, A. Hoseinkhani, B. Ghasemi Panahi, G.R. Dehghan and I. Ashrafi. 2015. Effect of Origanum vulgare ethanol extract on quality parameters and malondialdehyde concentration of cryopreserved Holstein bull sperm. Animal Science Research 25(1): 1-11.
22. Ghanbari, E., M. Khazaei and F. Yosefzaei. 2017. The restorative effect of prosopis farcta on fertility parameters and antioxidant status in diabetic rats. Journal of Babol University of Medical Sciences 19(5): 53-60.
23. Ghasemi, A. 2009. Iranian medicinal and aromatic herbs (Recognizing and investigating their effects). Islamic Azad University, Shahrekord, 491.
24. Gill-Guzman, E., M. Ollero, M.C. Lopez, R.K. Sharma and J.G. Ala varez. 2001. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of matauration. Human Reproduction 16(9): 1922-30.
25. González, N., L. Gil, F. Martinez, C. Malo, R. Cano, Mur, P. and E. Espinosa. 2010. Effect of natural antioxidant rosemary in canine soya freezing extender. Reproduction in Domestic Animals 45: 88.
26. Halliwell, B. and J.M. Gutteridge. 2015. Free radicals in biology and medicine. Oxford University Press, USA, 330-332.
27. Hsieh, Y.Y., Y.L. Sun, C.C. Chang, Y.S. Lee, H.D. Tsai and C.S. Lin. 2002. Superoxide dismutase activities of spermatozoa and seminal plasma are not correlated with male infertility. Journal of Clinical Laboratory Analysis 16(3): 127-131.
28. Ji, G., A. Gu, Y. Wang, C. Huang, F. Hu, Y. Zhou, L. Song and X. Wang. 2012. Genetic variants in antioxidant genes are associated with sperm DNA damage and risk of male infertility in a Chinese population. Free Radical Biology and Medicine. 52(4): 775-780.
29. Karamian, R., M. Asadbeigy and S. Yari. 2018. Antioxidant activity of glycyrrhiza glabra L. extract and protective effect of its leaf extract on ethanol-induced nephrotoxicity in male rats. Scientific Journal of Ilam University of Medical Sciences 26(4): 1-12.
30. Kazemian, S., A. Karimi, A. Pilevariyan and A. Ghandi. 2017. The effect of hydro-alcoholic shallots extract on testis and spermatogenesis in Balb/C Mice. Journal of Zanjan University Medical Science 109(25): 50-63.
31. Khaki, A., M. Nouri, F. Fathiazad, H.R. Ahmadi Ashtiani, H. Rastgar and S. Rezazadeh. 2009. Protective effects of quercetin on spermato-genesis in streptozotocin induced diabetic rat. Journal of Medical Plants 8: 57-64.
32. Koca, Y.O., O.L. Ozdal, M. Celik, S. Unal and N. Balaban. 2003. Antioxidant activity of seminal plasma in fertile and infertile men. Archives of Andrology 49(5): 355-359.
33. Kunzle, R., M.D. Mueller, W. Hanggi, M.H. Birkhauser, H. Drescher and N.A. Bersinger. 2003. Semen quality of male smokers and nonsmokers in infertile couples. Fertility and Sterility 79(2): 287-291.
34. Kuriakose, G. C. and M.G. Kurup. 2010. Antioxidant and hepatoprotective activity of Aphanizomenon flos-aquae Linn against paracetamol intoxication in rats. Indian Journal of Experimental Biology 48(11): 1123-1130.
35. Love, C. C., T.L. Blanchard, S.P. Varner, J. Voge and S. Bliss. 2012. Effect of daily semen centrifugation and resuspension on the longevity of equine spermquality following cooled storage. Theriogenology 77: 1911-1917.
36. Mahdiani, E., H. Khadem Haghighian, M. Javadi, A.A. Karami and M. Kavianpour 2018. Effect of Carob (Ceratonia siliqua L.) oral supplementation on changes of semen parameters, oxidative stress, inflammatory biomarkers and reproductive hormones in infertile men. Scientific Journal of Kurdistan University of Medical Science 95: 56-66.
37. McCord, J.M. and I. Fridovich. 1969. Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry 244: 6049-6055.
38. Mohajeri, D., Y. Doustar and Gh. Mousavi. 2012. Protective and antioxidant activities of turnip root ethanolic extract against cisplatin induced hepatotoxicity in rats. Zahedan Journal of Research in Medical Sciences 13(9): 8-15.
39. Mozaffarian, V. 2004. Trees and shrubs of Iran. Farhang Moaser Publication.
40. Mruk, D.D. and C.Y. Cheng. 2000. In vitro regulation of extracellular superoxide dismutase in sertoli cells. Life Science 67(2): 133-145.
41. Murawski, M., J. Saczko, A. Marcinkowska, A. Chwilkowska, M. Grybos and T. Banas. 2007. Evaluation of superoxide dismutase activity and its impact on semen quality parameters of infertile men. Folia Histochemica et Cytobiologica. 45: 123-126.
42. Naseri, M., M. Heydari Nasrabadi, P. Khodarahmi, F. Ahmadi, P. Mojibi and H. Abotalebei. 2011. Study of the effect of Fumaria parviflora alcoholic extract on spermatogenesis in male rats. New Cellular and Molecular Biotechnology Journal 1(2): 61-65.
43. Puntarulo, S. 2005. Iron, oxidative stress and human health. Molecular Aspects of Medicine 26(4): 299-312.
44. Sarbishegi, M. and O. Khajavi. 2018. Effects of hydro-alcoholic extract of withania coagulans on antioxidant status and sperm parameters following testosterone administration in rat. Journal of Ilam University of Medical Sciences 26(2): 11-20.
45. Sato, K., K. Sueoka, R. Tanigaki R., H. Tajima, A. Nakabayashi, Y. Yoshimura and Y. Hosoi. 2010. Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice. Journal of Assisted Reproduction and Genetics 27(8): 501-508.
46. Shamsi, M.B., S. Venkatesh, R. Kumar, N.P. Gupta, N. Malhotra, N. Singh, S. Mittal, S. Arora, D.S. Arya, P. Talwar and R.K. Sharma. 2010. Antioxidant levels in blood and seminal plasma and their impact on sperm parameters in infertile men. Indian Journal of Biochemistry and Biophysics 47(1): 38-43.
47. Sugioka, K., M. Nakano, H. Totsune, H. Minakami, S. Tero-Kubota and Y. ikegami. 1998. Mechanism of O2-generation in reduction and oxidation cycle of ubiquinones in a model of mitochindrial electeron transport systems. Biochimica et Biophysica Acta 936(3): 377-385.
48. Yan, L., J. Liu, S. Wu, S. Zhang, G. Ji and A. Gu A. 2014. Seminal superoxide dismutase activity and its relationship with semen quality and SOD gene polymorphism. Journal of Assisted Reproduction and Genetics 31(5): 549-554.
49. Zanganeh, Z., M. Zhandi, A. Zare Shahneh, A. Najafi, M. Mahdi Nabi and A. Mohammadi-Sangcheshmeh. 2013. Does rosemary aqueous extract improve buck semen cryopreservation? Small Ruminant Research 114(1): 120-125.

فایل ها

سابقه مقاله

  • تاریخ دریافت: 29 تیر 1398
  • تاریخ بازنگری: 20 مرداد 1398
  • تاریخ پذیرش: 16 شهریور 1398

هم رسانی

ارجاع به این مقاله

آمار