تاثیر زمان‌های مختلف پس از زایش بر شاخص‌های سوخت و ساز چربی در بافت چربی گاو هلشتاین

نوع مقاله : مقاله کامل

نویسندگان

گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران، ایران

چکیده

بافت چربی نقشی مهم در برقراری دوره شیردهی موفق و به دنبال آن تولیدمثل مطلوب دارد. هدف از پژوهش حاضر بررسی تغییرات بیان ژن‌های مرتبط با سوخت و ساز چربی در بافت چربی گاوهای هلشتاین در زمان‌های مختلف پس از زایش بود. گاوها (16=n) با میانگین تولید شیر بیشتر از30 کیلوگرم در روز، نوبت زایش (2/1±1/3) و نمره وضعیت بدنی (2/0±25/3) انتخاب و با جیره‌ای مشابه از زمان زایش تا روز 50 شیردهی تغذیه شدند. بیوپسی از بافت چربی زیرپوستی ناحیه پین در روزهای 21 و 42 شیردهی انجام شد. RNA کل استخراج و برای بررسی بیان نسبی ژن‌های مرتبط با بتا اکسیداسیون (CPT1A, ACOX1)، ساخت چربی (SREBP1C، LPL) و تجزیه چربی (ATGL، LIPE) از دستگاه Real-time PCR استفاده شد. نتایج نشان داد بیان نسبی ژن‌های مرتبط با اکسیداسیون، ACOX1 (03/3 برابر) و CPT1A (99/2 برابر) تحت تاثیر زمان بوده و با افزایش روزهای شیردهی افزایش یافت (05/0>P). در بین ژن‌های بررسی شده، بیان نسبی ژن‌های مرتبط با تجزیه چربی، LIPE (54/ 4 برابر) و ATGL (11/7 برابر) افزایش معنی‌داری نشان داد. بیان نسبی ژن‌های مرتبط با ساخت چربی، LPL (75/7 برابر) و SREP1C (61/2 برابر) با افزایش روزهای شیردهی به‌صورت معنی‌داری کاهش یافت. به‌طور کلی نتایج پژوهش حاضر نشان داد بیومارکرهای سوخت و ساز چربی در بافت چربی تحت تاثیر زمان‌های مختلف پس از زایش قرار گرفت. 

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Different Times Postpartum on Lipid Metabolism Markers in Adipose Tissue of Holstein Cows

نویسندگان [English]

  • E. Dirandeh
  • Y. Rezaeian
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran
چکیده [English]

Our objective was to determine the changes in expression of genes related to lipid metabolism in adipose tissue at 20 and 42 d in milk (DIM) in Holstein cows.  Cows (n= 16) were randomly selected and fed the same total mixed ration (TMR) from calving date to 50 DIM. Subcutaneous adipose tissue (AT) biopsies were collected from the tail-head region at 20 and 42 d relative to calving (d 0) as previously described by Sumner and McNamara (2007). The samples were immediately frozen in liquid nitrogen and then transferred to a − 80 °C freezer until analysis. Real-time PCR was conducted in an ABI Prism 7300 instrument (Rotor gene 3000, Corbett Life Science, Concorde, NSW, Australia) and used to measure gene expression related to beta-oxidation (ACOX1= acylcoenzyme A oxidase, CPT1A= carnitine palmitoyltransferase 1A), lipogenesis (SREBP1C= sterol regulatory element binding protein, LPL = lipoprotein lipase) and, lipolysis (ATGL= adipose triglyceride lipase, LIPE = hormone-sensitive lipase) by Real-time PCR. Results showed that genes that have role in the control offatty acid oxidation (CPT1A, ACOX1) were affected by time and enhances with increasing days in milk (P< 0.05). Among those genes significantly increasing in expression were those controlling lipolysis, including ATGL (52%) and LIPE (23%). Genes coding for enzymes controlling lipogenesis significantly decreased, including SREBP1C (−25%) and LPL (−48.4%). Lipid metabolism biomarkers in adipose tissue of Holstein cows affected by different time postpartum.

کلیدواژه‌ها [English]

  • Adipose tissue
  • Gene expression
  • Holstein cows
  • PCR

مراجع

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تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
دوره 33، شماره 3
مهر 1399
صفحه 84-91

فایل ها

سابقه مقاله

  • تاریخ دریافت: 16 تیر 1398
  • تاریخ بازنگری: 12 مرداد 1398
  • تاریخ پذیرش: 19 مرداد 1398

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