بهبود بازده اسپرم منجمد در قوچ با استفاده از آنتی اکسیدان گلوتاتیون

نوع مقاله : مقاله کامل

نویسندگان

1 موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

2 گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

هدف از اجرای این طرح بهبود بازده باروری اسپرم در گوسفند با افزودن آنتی‌اکسیدان‌ گلوتاتیون به محیط انجماد بود. در این آزمایش اسپرم از 5 رأس قوچ نژاد زل جمع‌آوری به رقیق‌کننده‌های حاوی صفر، 5/0، 1، 2، 4 و 8 میلی‌مولار گلوتاتیون اضافه و سپس منجمد شدند. در مرحله ارزیابی آزمایشگاهی کیفیت اسپرم، اسپرم‌های منجمد مورد ارزیابی آزمایشگاهی قرار گرفتند و فراسنجه‌های حرکتی، زنده‌مانی، سلامت غشا، مورفولوژی، سلامت آکروزوم، فعالیت میتوکندری و میزان لیپید پراکسیداسیون مورد ارزیابی قرار گرفتند. نتایج نشان داد که استفاده از 4 میلی مولار گلوتاتیون منجر به بهبود جنبایی کل، جنبایی پیشرونده، سلامت غشا، فعالیت میتوکندری، سلامت آکروزوم، زنده مانی و کاهش آپوپتوزیس و پراکسیداسیون لیپیدهای غشایی در اسپرم منجمد قوچ پس از یخگشایی شد. استفاده از تیمار 8 میلی مولار گلوتاتیون منجر به کاهش کیفیت اسپرم پس از یخگشایی شد و استفاده از آنتی اکسیدان گلوتاتیون تاثیری بر مورفولوژی اسپرم پس از فرایند انجماد-یخگشایی نداشت. در نتیجه استفاده از تیمار 4 میلی مولار آنتی اکسیدان گلوتاتیون می‌تواند راهکاری در جهت بهبود بازده باروری اسپرم قوچ پس از فرایند انجماد-یخگشایی باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Improvement of Ram’s Frozen Sperm Quality Using Glutathione Antioxidant

نویسندگان [English]

  • R. Masoudi 1
  • A. Z. Shahneh 2
  • N. Asadzadeh 1
1 Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
2 Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The aim of this study was improvement of ram frozen semen efficiency using addition of glutathione antioxidant to freezing medium. In this experiment, semen samples were collected from five Zell rams and add to the extender containing 0, 0.5, 1, 2, 4 and 8 mM glutathione. In sperm quality evaluation, sperm samples were evaluated and motion parameters, membrane integrity, morphology, acrosome integrity, mitochondria activity, viability and lipid peroxidation were assessed. The results showed using 4 mM glutathione resulted in improving of sperm total and progressive motility, membrane integrity, mitochondria activity, acrosome integrity, and viability as well as reduction of apoptosis rate and lipid peroxidation. Using the treatment of 8 mM glutathione caused to reduction of sperm quality after thawing and the antioxidantd did not have any effect on sperm morphology. In conclusion, using 4 mM glutathione in freezing extender could be a suitable method to improve ram sperm reproductive performance after freeze-thaw process.

کلیدواژه‌ها [English]

  • Sperm freezing
  • Sperm evaluation
  • Glutathione
  • Ram
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