بررسی اثر سطوح مختلف باکتری لاکتوباسیلوس اسیدوفیلوس بر پاسخ استرس اکسیداتیو و فراسنجه‌های بیوشیمیایی سرم خون ماهی قزل‌آلای رنگین‌کمان در مواجهه با فلز سرب در جیره غذایی

نوع مقاله : مقاله کامل

نویسندگان

1 استادیار، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 دانشجوی دکترای بهداشت آبزیان، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 دکترای بهداشت آبزیان، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

4 دانشجوی دکتری عمومی دامپزشکی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

استفاده از ترکیبات فراسودمند مانند پروبیوتیک‌ها می‌تواند در بهبود عملکرد آبزیان موثر باشد. هدف از مطالعه حاضر تاثیر سطوح مختلف باکتری پروبیوتیکی i(La) Lactobacillus acidophilusروی برخی از فاکتورهای بیوشیمیایی و آنتی‌اکسیدانی سرم خون ماهی قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) پس از مسمومیت با فلز سرب در جیره غذایی بوده است. تعداد 375 قطعه ماهی قزل‌آلا بطور تصادفی به پنج گروه در سه تکرار تقسیم شدند. گروه‌های یک، دو و سه به ترتیب از ابتدا تا انتهای آزمایش با جیره‌حاوی 106×5، 107×5 و 108×5 CFU/gr باکتری پروبیوتیکی تغذیه شدند. گروه چهارم (کنترل) در تمام دوره با جیره فاقد پروبیوتیک و بدون فلز سرب تغذیه شد. گروه پنجم به مدت 45 روز با جیره فاقد پروبیوتیک و سپس (به مدت 21 روز) همراه با تیمارهای پروبیوتیکی تا انتهای آزمایش با جیره حاوی µg/kg 500 نیترات سرب تغذیه شد. خونگیری از ماهیان در روزهای صفر، 45، 52، 59 و 66 آزمایش انجام شد. نتایج نشان داد که در گروه دو بعد از 45 روز مصرف پروبیوتیک مقادیر گلوکز، فسفر، ALP و SOD افزایش و مقادیر کلسترول و تری‌گلیسرید کاهش معنی‌داری نسبت به گروه کنترل داشته است (05/0 > p). پس از مواجهه با سرب مقادیر LDH در روز 59 و مقادیر ALP در تمامی روزها در گروه دو نسبت به گروه پنج بطور معنی‌داری کاهش یافت (05/0 >p). همچنین سطح آنزیم کاتالاز و SOD در گروه دو نسبت به گروه پنج در روز 66 افزایش معنی‌داری داشته است (05/0 > p). نتایج این مطالعه نشان داد که باکتری La بعنوان یک پروبیوتیک می‌تواند در بهبود شاخص‌های سرمی ماهی قزل‌آلا قبل و پس از مواجهه با فلز سرب نقش داشته باشد. همچنین غلظت 107×5 CFU/gr پروبیوتیک روی شاخص‌های سرمی تاثیر بهتری داشته است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of effect of variouslevels of Lactobacillus acidophilus on serum oxidative stress response and biochemical parameters of rainbow trout (Oncorhynchus mykiss) exposed to lead in diet

نویسندگان [English]

  • T. Mohammadian 1
  • R. Ghanei-Motlagh 2
  • S.S. Hosseini 3
  • S. Robatkarimi 4
  • M. Emam 4
  • N. Alijani 4
  • H. Bakhshi 4
1 Assistant professor, Department of clinical sciences, Faculty of veterinary medicine, Shahid chamran university of Ahvaz, Ahvaz, Iran.
2 PhD student of Aquatic animal health, Faculty of veterinary medicine, Shahid chamran university of Ahvaz, Ahvaz, Iran.
3 PhD of Aquatic animal health, Faculty of veterinary medicine, Shahid chamran university of Ahvaz, Ahvaz, Iran.
4 DVM student, Faculty of veterinary medicine, Shahid chamran university of Ahvaz, Ahvaz, Iran.
چکیده [English]

Application of functional ingredients like probiotics could be effective in promotiong of aquatic animals performance. The aim of this study was to investigate the effect of various levels of probiotic bacterium Lactobacillus acidophilus on some serum biochemical and antioxidant factors of rainbow trout (Oncorhynchus mykiss) following dietary lead poisoning. 375 trout fish were randomly divided into five groups in three replications. Groups 1, 2 and 3 were respectively fed with diets containing 5 × 106, 5 × 107 and 5 × 108 CFU / g probiotic bacteria from beginning to end of the experiment. Group 4 (control) was fed witha diet free of probiotic and lead. Group 5 was fed with probiotic-free diet for 45 days and then with a diet containing 500 μg / kg lead nitrate (for 21 days) to end of the experiment similar to probiotic-consuming treatments. Bleeding of fish was done on days 0, 45, 52, 59 and 66. The results showed that levels of glucose, phosphorus,ALP and SOD were significantly higher and cholesterol and triglyceride levels were significantly lower (p <0.05)in group 2 compared to control group after 45 days probiotic consumption. After lead exposure, LDH values on day 59 and ALP values in all days were significantly lower in group 2 compared to group 5 (p <0.05). Also, catalase and SOD levels were significantly higher in group 2 than group 5 on day 66 (p <0.05). The results of this study revealed that Lactobacillus acidophilus,as a probiotic,can be effective in improving the blood serum indices of trout before and after lead exposure. Also, level of 5 × 107 CFU / g of probiotic had a better effect on the serum parameters.

کلیدواژه‌ها [English]

  • Blood serum
  • Lead metal
  • Rainbow trout
  • Lactobacillus acidophilus

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تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
دوره 31، شماره 4
دی 1397
صفحه 91-105

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سابقه مقاله

  • تاریخ دریافت: 28 فروردین 1397
  • تاریخ بازنگری: 18 تیر 1397
  • تاریخ پذیرش: 24 تیر 1397

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