مطالعه‌ی آلودگی به انگل کریپتوسپوریدیوم در سگ‌های خانگی و ولگرد شهرستان‌های تبریز و ارومیه

نوع مقاله: مقاله کامل

نویسندگان

1 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

2 گروه بیماری های درونی و کلینیکال پاتولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران.

چکیده

تک‌یاخته کریپتوسپوریدیوم یکی ازتک‌یاخته‌های انگلی روده‌ای مهم در حیوانات و قابل انتقال به انسان با انتشار جهانی است. هدف مطالعه‌ی فراوانی آلودگی کریپتوسپوریدیوم در سگ‌های شهرستان تبریز و ارومیه بود. به این منظور از مدفوع 150 قلاده سگ ولگرد و 150 قلاده سگ خانگی نمونه‌برداری به روش تصادفی ساده انجام شد. برای جدا سازی و تخلیص اووسیست‌های کریپتوسپوریدیوم از روش شناورسازی و گرادیانت سوکروز و پرکول استفاده گردید و به روش زیل- نیلسن اصلاح‌شده رنگ‌آمیزی شدند. فراوانی کلی آلودگی 1 درصد (300 : 3) با توزیع فراوانی 67/0 درصد (150 : 1) و 33/1 درصد (150 : 2)، به ترتیب، در سگ‌های ولگرد تبریز و ارومیه بود ولی در سگ‌های خانگی آلودگی یافت نشد. بیشترین شیوع آلودگی در سگ‌های زیر یک سال بود. قوام مدفوع در نمونه‌های آلوده اسهالی و شل بود. فراوانی آلودگی در فصول مختلف معنی‌دار نبود. نتایج این مطالعه نشان داد که آلودگی کریپتوسپوریوم در مناطق تحت مطالعه با فراوانی پایینی حضور دارد که نیازمند مطالعه تکمیلی برای تعیین تنوع گونه‌ای و ژنوتیپ انگل به عنوان یک عامل بیماری‌زای مشترک در میان جمعیت دامی و انسانی می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

A comparative study on Cryptosporidium infection in household and stray dogs in Urmia and Tabriz municipalities, Iran

نویسندگان [English]

  • A. Khanzadeh-Karvigh 1
  • M. Yakhchali 1
  • M. Hadian 2
1 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Internal diseases and Clinical pathlogy, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
چکیده [English]

Cryptosporidium is an important intestinal pathogenic protozoan of livestock and human worldwide. The present study was carried out to compare Cryptosporidium infection in dogs of Urmia and Tabriz municipalities, Iran. A total of 300 fecal samples were collected from stray dogs and domestic dogs of the regions. Cryptosporidium oocysts were removed using floatation method and purified with sucrose and Percoll gradients. Finally, the slides were stained using modified Ziehl-Neelsen technique. Microscopically, overall prevalence was 1% (3/300) with distributions of 0.67% (1/150) and 1.33% (2/150) in Tabriz and Urmia, respectively. There was no cryptosporidial infection in household dogs. The highest infection rate was found in stray dogs less than 1 year old (0.66%, 1/150). In the infected dogs, the consistency of feces was diarrheal and soft. There was no significant association between season and prevalence. The results indicated that Cryptosporidium infection existed in dogs of Tabriz and Urmia municipalities with low prevalence. It is necessary as a zoonotic agent to do further investigations on Cryptosporidium species diversity and genotypes in human and animal populations of northwestern Iran.

کلیدواژه‌ها [English]

  • Cryptosporidium
  • Dog
  • Tabriz
  • Urmia
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