تشخیص آسیب‌شناسی و ملکولی ویروس آبله پرندگان در مرغان بومی شهر خرم‌آباد

نوع مقاله: مقاله کامل

نویسندگان

1 گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه لرستان

2 گروه پاتوبیولوژی دانشکده دامپزشکی دانشگاه لرستان

3 گروه علوم درمانگاهی دانشکده دامپزشکی دانشگاه آزاد اسلامی واحد کرج

چکیده

آبله یک بیماری ویروسی متداول و واگیردار در گونه‌های مختلف پرندگان اهلی و وحشی با گسترش جهانی است که عموماً برای گونه‌های مختلف پرندگان اختصاصی میزبان است. در مطالعه حاضر، همه‌گیری‌های آبله پرندگان در مرغان بومی حومه خرم‌آباد با علائم بالینی نظیر بی‌اشتهایی، کز کردن، ژولیدگی پرها، ضایعات به صورت ندول‌های زگیل مانند بر روی تاج، ریش و اطراف پلک‌ها، روی سطح دهان، حلق و اپیگلوت با میزان ابتلای 50 درصد و تلفات پنج تا 20 درصد مورد بررسی قرار گرفتند. در آزمایش هیستوپاتولوژی ضایعاتی از قبیل دژنرسانس، واکوئلدار شدن و هیپرپلازی سلول‌های پوششی و گنجیدگی‌های ائوزینوفیلیک درون سیتوپلاسمی (بولینجر بادی) در سلول‌های مبتلا مشاهده گردید. در آنالیز فیلوژنی جدایه‌های Fowl pox virus تعیین توالی شده در مطالعه حاضر دور از دیگر جدایه‌های ایران و در کنار جدایه‌هایی از تانزانیا، مصر و آلمان قرار گرفت. نتایج این مطالعه نشان داد که آگاهی از وضعیت وقوع آبله در مرغان بومی با توجه به آسیب این بیماری به اقتصاد مناطق روستایی و نقش آنها به عنوان منبع بیماری برای گله‌های طیور صنعتی واجد اهمیت زیادی است.

کلیدواژه‌ها


عنوان مقاله [English]

Pathological and molecular diagnosis of avian pox in backyard chickens in Khorram Abad

نویسندگان [English]

  • Hasan Norouzian 1
  • Ghasem Farjanikish 2
1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
2 Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
چکیده [English]

Avian pox is a world-wide, contagious and common viral disease of numerous domestic and wild bird species and is generally host specific for different species of birds. In the present study, avian pox infection was identified in backyard chickens of Khorramabad with clinical signs including anorexia, depression and ruffled feathers and morbidity and mortality rates of 50% and 5 to 20%, respectively in affected flocks. On clinical examination, the lesions were observed as wart-like nodules with different sizes on the comb, wattle and around the eyelids. In addition, in some affected birds, fibrinonecrotic lesions in oral mucosa, pharynx and epiglottis was observed. Histopathologic examination of lesions revealed degeneration, vacuolization and hyperplasia of epithelial cells associated with intracytoplasmic eosinophilic inclusion bodies (Bollinger bodies). Based on the phylogenetic analysis, the isolated fowl poxvirus was classified in a different subclade far from other Iranian isolates and near to isolates from Tanzania, Egypt and Germany. The evaluation of avian pox situation in backyard chickens is very important because of its economic losses in rural areas and their role as sources of infection in commercial poultry.

کلیدواژه‌ها [English]

  • Avian pox
  • Backyard chickens
  • PCR
  • Histopathology
  • Phylogenetic analysis

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