تاثیر گرسنگی بر سطوح گلیکوژن تری گلیسیرید، کلسترول و آنزیم‌های آمینوترانسفراز کبد بچه‌ماهی کفال خاکستری (Mugil cephalus)

اکبری, پریا; بیابانی, سعیده; سلیمانی‌فر, خالد

doi:10.22092/vj.2017.109889

تاثیر گرسنگی بر سطوح گلیکوژن تری گلیسیرید، کلسترول و آنزیم‌های آمینوترانسفراز کبد بچه‌ماهی کفال خاکستری (Mugil cephalus)

نوع مقاله : مقاله کامل

نویسندگان

دانشگاه دریانوردی و علوم دریایی چابهار، دانشکده علوم دریایی، گروه شیلات

10.22092/vj.2017.109889

چکیده

در صنعت آبزی‌پروری، توجه به کیفیت ماهیان پرورشی حائز اهمیت است و یکی از فاکتور‌های موثر بر تعیین کیفیت تولید، میزان تغذیه و گرسنگی می‌باشد. این تحقیق، به‌منظور تعیین اثر گرسنگی بر گلیکوژن، تری‌گلیسرید، کلسترول و فعالیت آنزیم‌های آمینوترانسفراز کبد بچه‌ماهی کفال خاکستری (Mugil cephalus) انجام گردید. 180عدد بچه‌ماهی کفال خاکستری با میانگین وزنی 10/1±62/21 گرم به‌صورت تصادفی به دو گروه تغذیه شده که دو بار در روز (8 صبح و 4 بعد ازظهر) غذادهی شدند و تغذیه نشده (گرسنه) با 3 تکرار تقسیم شدند (30 قطعه در هر تکرار). نمونه‌برداری از هر گروه به‌ترتیب در 0، 1، 2، 3 و 4 هفته پس از شروع آزمایش، با صید 5 قطعه ماهی از هر تکرار در هر نوبت انجام شد. تمام فاکتور‌های بیوشیمیایی از طریق دستگاه اتوآنالایزر اندازه‌گیری شدند. مقادیر کلسترول، آلانین آمینوترانسفراز، آسپارتات آمینوترانسفراز و تری‌گلیسرید در بین گروه تغذیه شده و تغذیه نشده در طول دوره آزمایش، اختلاف معنی‌داری را نشان ندادند. میزان گلیکوژن در گروه تغذیه نشده کاهش معنی‌داری در مقایسه با گروه تغذیه نشده نشان داد (05/0>P). نتایج به‌دست آمده نشان می‌دهد که گلیکوژن به‌عنوان اولین منبع انرژی در ماهی کفال خاکستری مورد استفاده قرار می‌گیرد.

کلیدواژه‌ها

20.1001.1.24235407.1396.30.3.15.3

عنوان مقاله [English]

Effect of starvation on liver glycogen, triglyceride cholesterol and aminotransferase enzymes in grey mullet, (Mugil cephalus) fingerling

نویسندگان [English]

P. Akbary
S. Biabani
Kh. Solimani Far

Chabahar Maritime University, Faculty of Marine Sciences, Fisheries group, Iran.

چکیده [English]

The quality of farmed fish is becoming increasingly important within the aquaculture industry and levels of feeding and starvation are crucial factors that determine product quality. In this study the effect of starvation on glycogen, triglyceride, cholesterol and aminotransferase enzyme activities of liver in grey mullet fingerling was investigated 180 fingerling were randomly distributed into six tanks (three tanks for each of two experimental groups, fed twice a day (8 AM and 16 PM) and starved) with 30 fish in each tank. The experiment consisted of five samplings performed 0,1,2,3 and 4 weeks after the onset of the experiment Levels of total triglyceride, total cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and glycogen were analyzed using an automatic biochemistry analyzer. No significant differences in levels of total cholesterol, triglyceride, AST and ALT of liver were detected between fed and starved groups at any sampling time throughout the experiment. In contrast, liver glycogen levels were significantly lower in starved fish than in fed fish from day 7 onwards. The present results indicate that glycogen is used as the first energy resource in Mugil cephalus.

کلیدواژه‌ها [English]

Mugil cephalus
LIVER GLYCOGEN
Food deprivation
Liver aminotrasferase

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