بررسی فراوانی بروسلا آبورتوس در گاوهای هلشتاین شمال غرب ایران با استفاده از روش‌های سرولوژی و PCR

نوع مقاله : مقاله کامل

نویسندگان

1 گروه مامایی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

2 گروه بیماری‌های درونی و کلینیکال پاتولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

3 دانش آموخته دکتری دامپزشکی، گروه مامایی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

چکیده

بروسلوزیس یک بیماری مشترک با گسترش جهانی است که سبب سقط‌جنین و کاهش باروری در گاو می‌شود. بروسلوزیس همواره در ایران بروز بالایی دارد. هدف از مطالعه حاضر بررسی شیوع آلودگی به بروسلا آبورتوس در گاو‌های هلشتاین شمال غرب ایران با استفاده از روش‌های سرولوژی و PCR بود. در این مطالعه نمونه خون از 1692 راس از گاوهای شیری استان‌های آذربایجان غربی، آذربایجان شرقی و اردبیل (شمال غرب ایران) جمع‌آوری و سرم آن‌ها توسط سانتریفیوژ جدا گردید. نمونه‌های سرمی برای انجام آزمایش رزبنگال (RBT)، آزمایش 2-ME و روش PCR مورد استفاده قرار گرفت. از تعداد 1692 نمونه جمع آوری شده، 65/14 درصد آزمایش رزبنگال، 23/13 درصد آزمایش 2-ME و 98/21 درصد در آزمایش PCR مثبت بودند. این‌طور می‌توان نتیجه گرفت که شیوع بیماری بروسلوز در گاو‌های شیری این منطقه پایین بوده و از بین تمام تست‌های کمک تشخیصی، روش‌های مولکولی نظیر PCR برای تشخیص سویه‌های بروسلا در گاوهایی که در معرض سقط‌جنین هستند استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Prevalence of Brucella abortus in Holstein dairy cows in north west of Iran by using serological and PCR assays

نویسندگان [English]

  • A. Soleimanzadeh 1
  • Gh. Jalilzadeh-Amin 2
  • Ali Vaghar 3
1 Assistant Professor, Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Internal Medicine and Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 DVM graduate, Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
چکیده [English]

Bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reduced fertility in cows. The aim of this study was to determine prevalence of Brucella abortus in Holstein dairy cows in north west of Iran by using serological and PCR assays. In this study, blood samples were drawn from 1692 cows at the dairy farms of west Azerbaijan, east Azerbaijan and Ardabil provinces (North-West of Iran) and their sera separated by centrifugation. The serum samples were analyzed by Rose Bengal Test (RBT), 2-ME and multiplex PCR assays. From 1692 samples collected, 14.65% showed positive RBT and 13.23% showed positive 2-ME and 21.98% showed a positive reaction to the PCR test. In conclusion, all serological and particularly PCR tests are recommended for diagnosis of Brucella strains in cows subjected to abortion. It was concluded from the results that prevalence of brucellosis in cattle was low in this region.

کلیدواژه‌ها [English]

  • Brucella abortus
  • Serological
  • PCR
  • cow
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