بررسی فراوانی بروسلا آبورتوس در گاوهای هلشتاین شمال غرب ایران با استفاده از روش‌های سرولوژی و PCR

نوع مقاله: مقاله کامل

نویسندگان

1 گروه مامایی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

2 گروه بیماری‌های درونی و کلینیکال پاتولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

3 دانش آموخته دکتری دامپزشکی، گروه مامایی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

چکیده

بروسلوزیس یک بیماری مشترک با گسترش جهانی است که سبب سقط‌جنین و کاهش باروری در گاو می‌شود. بروسلوزیس همواره در ایران بروز بالایی دارد. هدف از مطالعه حاضر بررسی شیوع آلودگی به بروسلا آبورتوس در گاو‌های هلشتاین شمال غرب ایران با استفاده از روش‌های سرولوژی و PCR بود. در این مطالعه نمونه خون از 1692 راس از گاوهای شیری استان‌های آذربایجان غربی، آذربایجان شرقی و اردبیل (شمال غرب ایران) جمع‌آوری و سرم آن‌ها توسط سانتریفیوژ جدا گردید. نمونه‌های سرمی برای انجام آزمایش رزبنگال (RBT)، آزمایش 2-ME و روش PCR مورد استفاده قرار گرفت. از تعداد 1692 نمونه جمع آوری شده، 65/14 درصد آزمایش رزبنگال، 23/13 درصد آزمایش 2-ME و 98/21 درصد در آزمایش PCR مثبت بودند. این‌طور می‌توان نتیجه گرفت که شیوع بیماری بروسلوز در گاو‌های شیری این منطقه پایین بوده و از بین تمام تست‌های کمک تشخیصی، روش‌های مولکولی نظیر PCR برای تشخیص سویه‌های بروسلا در گاوهایی که در معرض سقط‌جنین هستند استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Prevalence of Brucella abortus in Holstein dairy cows in north west of Iran by using serological and PCR assays

نویسندگان [English]

  • A. Soleimanzadeh 1
  • Gh. Jalilzadeh-Amin 2
  • Ali Vaghar 3
1 Assistant Professor, Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
2 Department of Internal Medicine and Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 DVM graduate, Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
چکیده [English]

Bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reduced fertility in cows. The aim of this study was to determine prevalence of Brucella abortus in Holstein dairy cows in north west of Iran by using serological and PCR assays. In this study, blood samples were drawn from 1692 cows at the dairy farms of west Azerbaijan, east Azerbaijan and Ardabil provinces (North-West of Iran) and their sera separated by centrifugation. The serum samples were analyzed by Rose Bengal Test (RBT), 2-ME and multiplex PCR assays. From 1692 samples collected, 14.65% showed positive RBT and 13.23% showed positive 2-ME and 21.98% showed a positive reaction to the PCR test. In conclusion, all serological and particularly PCR tests are recommended for diagnosis of Brucella strains in cows subjected to abortion. It was concluded from the results that prevalence of brucellosis in cattle was low in this region.

کلیدواژه‌ها [English]

  • Brucella abortus
  • Serological
  • PCR
  • cow
1. Akbarmehr, J. and M. Ghiyamirad. 2011. Serological survey of brucellosis in livestock animals in Sarab City (East Azarbayjan province), Iran. African Journal of Microbiology Research 5(10):1220-1223.
2. Bricker, B.J., Ewalt, D.R., MacMillan, A.P., Foster, G. and S. Brew. 2000. Molecular characterization of Brucella strains isolated from marine mammals. Journal of clinical microbiology. 38(3):1258-1262.
3. Bricker, B.J. 2002. PCR as a diagnostic tool for brucellosis. Veterinary microbiology. 90(1): 435-446.
4. Doomari, H. 2009. A serological survey on cow brucellosis in Jiroft city. Journal of Veterinary Research. 64(3): 233-236. (In Farsi).
5. Doosti, A. and P.G. Dehkordi. 2011. Application of real-time PCR for identification and differentiation of Brucella abortus and Brucella melitensis in cattle. Bulgarian Journal of Veterinary Medicine. 14(2): pp.109-115.
6. Foster, G., Osterman, B.S., Godfroid, J., Jacques, I. and A. Cloeckaert. 2007. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. International journal of systematic and evolutionary microbiology. 57(11): 2688-2693.
7. Gholizadeh, S. S., Zali, M. H., Hashempour, A. and E. M. Ahmadi. 2013. Investigation of brucellosis in cattle and sheep in Urmia-Iran. Yüzüncü yıl Üniversitesi Veteriner Fakültesi Dergisi. 24(3): 133-134.
8. Hasani Tabatabaie, A.M and Firozi, R. 2016. Bacterial diseases of animals. 4th ed. pp. 310-520, Publication: Tehran University press, Iran. (In Farsi)
9. Huber, B., Scholz, H.C., Lucero, N. and H. J. Busse. 2009. Development of a PCR assay for typing and subtyping of Brucella species. International Journal of Medical Microbiology. 299(8): 563-573.
10. Ilhan, Z., Aksakal, A., Ekin, I.H., Gülhan, T., Solmaz, H. and S. Erdenlig. 2008. Comparison of culture and PCR for the detection of Brucella melitensis in blood and lymphoid tissues of serologically positive and negative slaughtered sheep. Letters in applied microbiology. 46(3): 301-306.
11. Leal-Klevezas, D. S., Lopez-Merino, A. and J. P. Martinez-Soriano. 1994. Molecular detection of Brucella spp.: rapid identification of B. abortus biovar I using PCR. Archives of medical research. 26(3): 263-267.
12. Leuenberger, R., Boujon, P., Thür, B., Miserez, R., Garin-Bastuji, B., Rüfenacht, J. and K.D.C. Stärk. 2007. Prevalence of classical swine fever, Aujeszky's disease and brucellosis in a population of wild boar in Switzerland. Veterinary Record: Journal of the British Veterinary Association. 160(11).
13. Maadi, H., Moharamnejad, M. and M. Haghi. 2011. Prevalence of brucellosis in cattle in Urmia, Iran. Pakistan Veterinary Journal. 31(1): 81-82.
14. Moradi, G., Esmaiel Nasab, N., Ghaderi, E., Sofi Majidpour, M. and H. Salimzadeh. 2006. Brucellosis in Kurdistan Province from 1997 to 2003. Annals of Alquds Medicine. 2(1): 32-37.
15. Moreno, E., Cloeckaert, A. and I. Moriyón. 2002. Brucella evolution and taxonomy. Veterinary microbiology. 90(1): 209-227.
16. Nielsen, K. (2002). Diagnosis of brucellosis by serology. Veterinary microbiology. 90(1): 447-459.
17. O’Leary, S., Sheahan, M. and T. Sweeney. 2006. Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Research in veterinary science. 81(2): 170-176.
18. Ocholi, R.A., Kwaga, J.K.P., Ajogi, I. and J.O.O. Bale. 2004. Phenotypic characterization of Brucella strains isolated from livestock in Nigeria. Veterinary Microbiology. 103(1):47-53.
19. Samaha, H., Mohamed, T.R., Khoudair, R.M. and H.M. Ashour. 2009. Serodiagnosis of brucellosis in cattle and humans in Egypt. Immunobiology. 214(3): 223-226.
20. Silva, T.M.A.D., Oliveira, R.G.D., Mol, J.P.D.S., Xavier, M.N., Paixão, T.A.D., Cortez, A., Heinemann, M.B., Richtzenhain, L.J., Lage, A.P. and R.D.L. Santos. 2009. Etiologic diagnosis of bovine infectious abortion by PCR. Ciência Rural. 39(9): 2563-2570.
21. Verger, J.M., Grayon, M., Cloeckaert, A., Lefèvre, M., Ageron, E. and F. Grimont. 2000. Classification of Brucella strains isolated from marine mammalsusing DNA–DNA hybridization and ribotyping. Research in microbiology. 151(9): 797-799.
22. Yagupsky, P. and E.J. Baron. 2005. Laboratory exposures to Brucella and implications for bioterrorism. Emerging Infectious Disease Journal. 11(8): 1180-1185.
23. Youngquist, R. S and W. R. Threlfall. 2007. Current Therapy in Larg Animal Theriogenology. 2rd ed. Philadelphia: Saunders Company.
24. Zamanian, M., Hashemi Tabar, G.R., Rad, M. and A. Haghparast. 2015. Evaluation of different primers for detection of Brucella in human and animal serum samples by using PCR method. Archives of Iranian Medicine (AIM). 18(1): 44-50.