ارتباط بین برخی از فاکتورهای خونی، غلظت فاکتور نکروزکننده تومور آلفا و ناهنجاری‌‌های متابولیکی طی دوره‌‌ انتقال در گاوهای هلشتاین

نوع مقاله: مقاله کامل

نویسندگان

گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

هدف از پژوهش حاضر بررسی ارتباط بین برخی از فاکتورهای خونی، فاکتور نکروز کننده تومور آلفا (TNF-α) و ناهنجاری‌‌های متابولیکی طی دوره‌‌ انتقال در گاوهای هلشتاین بود. بدین منظور 50 رأس گاو هلشتاین آبستن با نمره وضعیت بدنی 75/0±5/3 و نوبت زایش سوم انتخاب شدند. خون‌گیری از دو هفته قبل از زایش تا دو هفته پس از زایش به‌‌صورت هفتگی انجام شد و غلظت پلاسمایی اسیدهای چرب غیراستریفه (NEFA)، بتاهیدروکسی بوتیرات (BHBA) و مقدار فعالیت آنزیم‌‌های آسپارتات آمینوترانسفراز (AST) و آلانین آمینو تراسفراز (ALT) در نمونه‌‌ها اندازه‌‌گیری و دام‌‌ها بر مبنای نتایج حاصل به دو گروه سالم و دارای ناهنجاری متابولیک تقسیم ‌‌شدند. نتایج نشان داد غلظت پلاسمایی TNF-αدر گاوهای دارای ناهنجاری متابولیک بیشتر از گاوهای سالم بود ولی بالعکس غلظت پلاسمایی انسولین و گلوکز در گاوهای دارای ناهنجاری متابولیک کمتر از گاوهای سالم بود. تاثیر زمان بر غلظت پلاسمایی گلوکز معنی‌‌دار بود به‌‌طوری‌‌که غلظت آن در هر دو گروه تا زمان زایش افزایش و سپس تا روز 14+ کاهش یافت. غلظت پلاسمایی گلوکز در روز 7+ و 14+ در گاوهای سالم بیشتر از گاوهای دارای ناهنجاری متابولیک بود. غلظت پلاسمایی فاکتور نکروزکننده تومور آلفا در کل دوره پژوهش در گاوهای بیمار بیشتر از گاوهای سالم بود. به‌‌طور کلی نتایج پژوهش حاضر نشان داد با توجه به غلظت بیشتر فاکتور نکروزکننده تومور آلفا قبل از زایش در گاوهای مستعد به ناهنجاری‌‌های متبولیک، اندازه‌‌گیری غلظت فاکتور نکروزکننده تومور آلفا قبل از زایش می‌‌تواند در شناخت گاوهای مستعد ابتلا به ناهنجاری‌‌های متابولیک و استفاده از راهکارهای مناسب برای کاهش شدت وقوع آن کمک کند.

کلیدواژه‌ها


عنوان مقاله [English]

Association between blood metabolites, tumor necrosis factor alpha concentrations and metabolic disorders in Holstein cows during transition period

نویسندگان [English]

  • F. Mohseni
  • B. Shohreh
  • Essa Dirandeh
  • Z. Ansari Pirsaraei
Department of Animal Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
چکیده [English]

The objectives of present study was to evaluate the association among blood metabolites, tumor necrosis factor (TNF)-alpha and metabolic diseases in Holstein cows during transition period. Pregnant multiparous Holstein cows (N = 50), with body condition scores (BCS) 3.5±0.75 and third parity were selected. Blood samples were collected weekly from 2 week prior to calving to 2 weeks after calving to determine plasma concentrations of non-esterifies fatty acids (NEFA), beta-Hydroxyl butyrate acid (BHBA) and activity of aspartate aminotransferase (AST) and alanine aminotrans-ferase (ALT) enzymes. According to above parameters cows divided into healthy (N =10) and diseases cow (n= 10) and plasma concentrations of glucose, insulin and TNF-α compared weekly from 2 week prior to calving to 2 weeks after calving.Results showed cows with metabolic disorders had higher plasma concentrations of NEFA, BHBA, AST and TNF-alpha compared to healthy cows (P < 0.05) but conversely plasma concentrations of glucose and insulin were lower in cows with metabolic disorders compared to healthy cows (P < 0.05). Plasma glucose concentration affected by time and increased from -14 to calving in both treatments and after that decreased until d +14. Glucose concentrations at d +7 and +14 were greater in healthy cows compared to cows with metabolic disorders. Plasma TNF-α concentration were greater in cows with metabolic disorders compared to healthy cows during whole esxperiment. In conclusion due to greater TNF-α concentration in cows with metabolic disorders before calving, using this item could be useful to predict cows suspicious to metabolic disorders after calving therefore we can use suitable strategies to prevent or decreased incidence of metabolic disorders.

کلیدواژه‌ها [English]

  • Fatty liver
  • Glucose
  • Holstein cow
  • ketosis
  • Tumor necrosis factor alpha
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