ارزیابی بیومکانیکی داربست سه بعدی زیستی تهیه شده از تاندون شترمرغ به عنوان گرافت

نوع مقاله : مقاله کامل

نویسندگان

گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

چکیده

هدف اصلی در مهندسی بافت ایجاد ساختاری مشابه ساختار طبیعی موجود در بدن موجود زنده، جهت ترمیم آسیب‌های بافتی است. همواره مشکلاتی در انجام پیوند بافت وجود داشته است. از جمله کمبود منابع بافتی مناسب و پس‌زدن ایمونولوژیک بافت‌های پیوند زده شده است؛ در مهندسی بافت یکی از جدیدترین روش‌ها به منظور کاهش واکنش ایمنی بدن نسبت به بافت پیوند زده شده، روش سلول‌زدایی بافت است. تعداد 12 نمونه تاندون آشیل شترمرغ در دو گروه سلول زدایی شده (گرافت) و نشده (کنترل) از شتر مرغ نر 2 تا 3 ماهه تهیه گردید. عمل سلول‌زدایی با سدیم دودسیل سولفات انجام شد. پس از ثبوت و طی روند معمول تهیه مقاطع بافتی، به منظور ارزیابی ساختار بافتی از رنگ آمیزی‌های هماتوکسیلین-ائوزین، پیکروسیروس قرمز و جهت بررسی میزان سلول‌زدایی و بقایای هسته از رنگ آمیزی DAPI استفاده گردید و ارزیابی استحکام بافتی به وسیله آزمون بیومکانیکی انجام شد. نتایج حاصل  از آن توسط Student-Test و Mann-Whithney با نرم افزار SPSS، در دامنه (05/0 ≥ p) مورد ارزیابی قرار گرفتند. در ارزیابی میزان سلول‌زدایی، 99% گرافت فاقد هسته و بقایای DNA بود. گروه کنترل در مقایسه  بازنوگرافت پس از طی فرآیند سلول زدایی، از نظر کیفیت کلاژنی فاقد اختلاف معنی‌دار و از نظر  نظم رشته‌ای دارای اختلاف معنی‌داری بود. در آزمون بیومکانیکی میزان تنش تفاوت معنی‌داری مشاهده نشد ولی در کرنش بافتی گرافت کاهش معنی‌داری را نشان داد. با ارزیابی خصوصیات ساختار بافتی و بیومکانیکی داربست سه بعدی تهیه شده در مقایسه با تاندون شترمرغ، مشخص شد این زنوگرافت دارای شرایط مناسب برای پیوند می‌باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Biomechanical evaluation of three-dimensional biological scaffold made from Ostrich tendons as graft

نویسندگان [English]

  • S. Hosseini
  • Sh. Hasanzadeh
  • A. Shahrooz
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
چکیده [English]

The main goal of tissue engineering design and create a structure similar to the natural structure of a tissue in vivo for repair tissue damages. Tissue transplantation, there are always problems, including a shortage of suitable tissue and immunological rejection of grafted tissue is in tissue engineering is one of the newest methods to reduce the immune response to transplanted tissue, is tissue decellularization methods (Acellular). After fixation and routine tissue  process, to evaluate the structure of tissue H&E stained, collagen quality assessment xenograft Picrosirius red stained , review and tracking the decellularization, detected  nuclei trace and any DNA remaining 4', 6'diamino-2-phenylindole-2HCl (DAPI) staining was used. Assess tissue strength by biomechanical test was performed. Biomechanical results by Student t-Test and Mann-Whithney (P≤0.05)was performed with SPSS software. Compared with ostrich tendonsand  matrix three-dimensional structure after the process of decellularization, the tissue density is somewhat reduced cohesion between collagen fibers were observed in some places a few miles away. Quality collagen stained by Picrosirius red that there is no difference between them. In assessing the amount of de-cells after staining DAPI 99% of the three-dimensional scaffold and remains free of nuclear DNA. Biomechanical testing of Stress, there was no difference in graft tissue but strain showed a significant decrease. Evaluation of properties of three-dimensional tissue structure made of scaffolding and comparison with ostrich tendons found this graft is the appropriate for graft.

کلیدواژه‌ها [English]

  • Graft
  • tendon
  • three-dimensional scaffold
  • Acellular
  • Ostrich

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تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
دوره 29، شماره 2
تیر 1395
صفحه 64-74

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سابقه مقاله

  • تاریخ دریافت: 18 شهریور 1394
  • تاریخ بازنگری: 18 فروردین 1395
  • تاریخ پذیرش: 09 آذر 1394

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