پاراتوبرکولوزیس در نشخوارکنندگان، جستجوی مولکولی بدنبال شواهد وجود مایکوباکتریوم ایویوم زیرگونه پاراتوبرکولوزیس Type I و Type II در ایران

تدین, کیوان; مصوری, نادر; کشاورز, روح اله; شاهمرادی, امیرحسین; قادری, رایناک; سخاوتی, محمد; مهره کش حقیقت, مریم

doi:10.22034/vj.2016.105749

پاراتوبرکولوزیس در نشخوارکنندگان، جستجوی مولکولی بدنبال شواهد وجود مایکوباکتریوم ایویوم زیرگونه پاراتوبرکولوزیس Type I و Type II در ایران

نوع مقاله : مقاله کامل

نویسندگان

¹ استادیار موسسه تحقیقات واکسن و سرم سازی رازی رئیس بخش

² دکترای تخصصی، استادیار مؤسسه تحقیقات واکسن و سرم سازی رازی رئیس بخش

³ دانشجوی دکترا مؤسسه تحقیقات واکسن و سرم سازی رازی

⁴ دکترای دامپزشکی مؤسسه تحقیقات واکسن و سرم سازی رازی

⁵ دکترای تخصصی مؤسسه تحقیقات واکسن و سرم سازی رازی معاون بخش

⁶ کارشناسی ارشد، دانش آموخته دکترا مؤسسه تحقیقات واکسن و سرم سازی رازی

10.22034/vj.2016.105749

چکیده

پاراتوبرکولوزیس یک نوع التهاب گرانولوماتوز مزمن و پیشرونده غیر قابل درمان روده می‌باشد که توسط مایکوباکتریوم ایویوم زیرگونه پاراتوبرکولوزیس (Mycobacterium avium subsp. paratuberculosis MAP) ایجاد می‌گردد. پاراتوبرکولوزیس در تمام جهان در میان نشخوارکنندگان دیده می‌شود. نخستین گزارشات پاراتوبرکولوزیس در ایران به دهه 1960 میلادی باز می‌گردد زمانی که بیماری در میان دام‌های وارداتی گاوداری شرکت نفت آبادان شناسایی گردید. در مطالعه حاضر تعداد 13 جدایه کلینیکی MAP شامل 4 جدایه گوسفندی و 3 جدایه بزی از استان اصفهان همراه با 3 جدایه گاوی از استان زنجان و 3 جدایه گاوی از استان البرز در کنار دو سویه واکسینال MAP 316F و MAP III & V توسط آزمایش های مستقل PCR-IS900، PCR-F57 به منظور تعیین هویت و PCR-Collins به منظور تعیین تیپ باکتری مورد بررسی قرار گرفتند. در نتیجه اجرای این بررسی از نظر تکنیکی امکان اجرای هر سه آزمایش با استفاده از یک پروتکل واحد PCR فراهم گردید و علاوه بر این هویت همه 15 جدایه کلینیکی و سویه واکسینال مورد بررسی به عنوان تیپ‌های گاوی (Type II) MAP مورد تائید قرار گرفت. مشاهده انحصاری این تیپ در میان باکتری‌های تحت بررسی علیرغم بزرگتر بودن جمعیت گله گوسفند و بز کشور در مقایسه با گله گاو از نظر اپیدمیولوژیکی جالب توجه بنظر می‌رسد.

کلیدواژه‌ها

20.1001.1.24235407.1395.29.1.11.8

عنوان مقاله [English]

Paratuberculosis in ruminants, a molecular search for traces of Mycobacterium avium subspecies paratuberculosis type I and type II in Iran

نویسندگان [English]

K., Tadayon, ¹
N., Mosavari, ²
R., Keshavarz, ³
AH., Shahmoradi, ⁴
R., Ghaderi, ⁵
M., Sekhavati, ³
M., Mohrekesh Haghighat, ⁶

¹ Assistant Professor of Razi Vaccine and Serum Research Institute, Alborz, Iran.

² Assistant Professor of Razi Vaccine and Serum Research Institute, Alborz, Iran.

³ PhD Student of Razi Vaccine and Serum Research Institute, Alborz, Iran.

⁴ Razi Vaccine and Serum Research Institute, Alborz, Iran.

⁵ Razi Vaccine and Serum Research Institute, Alborz, Iran.

⁶ Razi Vaccine and Serum Research Institute, Alborz, Iran.

چکیده [English]

Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (Map) is a progressive, chronic and non-curable granulomatous enteritis. Paratuberculosis affects ruminants worldwide. The earliest reports on paratuberculosis in Iran date back to 1960’s when the disease was diagnosed in imported animals of the Abadan Oil Company’s farm. In the work presented here 13 field MAP isolates including 4 ovine and 3 caprine isolates from Isfahan, 6 bovine isolates from Zanjan and Alborz provinces plus 2 vaccinal strains of 316F and III & V were subjected to PCR-IS900, PCR-F57 for identification and also PCR-Collins for typing. In consequence, while an identical PCR protocol was successfully developed to simultaneously perform all the three experiments, the 15 tested bacteria were all shown to be MAP Type II (cattle type). In epidemiological sense, when the size of national goat, sheep and cattle herds of Iran is taken into account, circulation of MAP Type II is intriguing.

کلیدواژه‌ها [English]

Mycobacterium avium subsp. paratuberculosis
IS900
F57
Type I
Type II

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