اثر حفاظتی گل همیشه بهار در بروز آسیب کبدی ناشی از تتراکلرید کربن در جوجه‌های گوشتی

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی دکترای دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 عضو هیات علمی دانشگاه فردوسی مشهد

3 کارشناسی ارشد دانشگاه فردوسی مشهد

چکیده

این مطالعه به‌‌ منظور بررسی اثر سطوح مختلف عصاره‌‌ روغنی ‌‌گل‌‌ همیشه‌‌ بهار بر آنزیم های کبدی و تغییرات هیستوپاتولوژی بافت کبد جوجه‌‌های گوشتی طی یک دوره 42 روزه انجام گرفت.تعداد 200 قطعه جوجه‌‌ گوشتی نر سویه‌‌ راس 308 در یک طرح کاملاَ تصادفی با پنج گروه و چهار تکرار و 10 پرنده در هر تکرار مورد آزمایش قرار گرفتند. تیمارهای آزمایشی شامل گروه کنترل منفی (فاقد افزودنی عصاره‌‌ گل‌‌ همیشه‌‌ بهار و تتراکلریدکربن)، کنترل مثبت {تتراکلریدکربن (یک میلی‌‌گرم بر کیلوگرم وزن بدن) و فاقد افزودنی عصاره‌‌ گل‌‌ همیشه‌‌ بهار}و سه تیمار دیگر شامل سطوح 150، 300 و 450 میلی‌‌گرم در کیلوگرم جیره‌‌ عصاره گل همیشه-بهار به‌‌ همراه تتراکلریدکربن بود. فراسنجه‌‌های خون (SGOT و SGPT)،  که در اثر تزریق تتراکلریدکربن، سطح‌‌شان در خون افزایش یافته بود، عصاره گل همیشه بهار توانست اثرات منفی ناشی از این ماده سمی را تقلیل دهد. وزن نسبی کبد در تیمارهایی که علاوه بر تتراکلریدکربن، سطوح مختلف عصاره گل همیشه بهار را دریافت کرده بودند، نسبت به تیمار دریافت کننده‌‌ی تتراکلریدکربن، به‌‌صورت معنی‌‌داری پایین تر
بود (05/0>P.).در شاخص‌‌های مربوط به سنجش سیستم ایمنی، سطح ایمنوگلوبولین G در تیماری که علاوه بر تتراکلریدکربن، سطح 300 میلی‌‌گرم عصاره گل همیشه‌‌بهار را دریافت کرده بودند نسبت به گروه کنترل منفی و غلطت 150 همیشه بهار به‌‌صورت معنی‌‌داری بالاتر بود (05/0>P). در بررسی تغییرات هیستوپاتولوژی کبد، مشاهده شد که گروه کنترل مثبت از لحاظ وضعیت سیاهرگ مرکزی، هپاتوسیت‌‌ها، سینوزوئیدها و تریادپورت بیشترین میزان آسیب بافتی و گروه کنترل منفی کمترین میزان آسیب‌‌دیدگی را داشتند. اما تیمارهایی که همراه با تتراکلریدکربن، عصاره‌‌ی گل همیشه‌‌ بهار را دریافت کرده بودند در مقایسه با گروه کنترل مثبت، بهبود بیشتری در بافت مشاهده شد (05/0>P). این نتایج نشان دهنده اثر حفاظتی عصاره گل همیشه‌‌ بهار بر  کبد می باشد که احتمالاً بواسطه فلاونویید های موجود در گیاه و توانایی آنها در مهار فعال سازی زیستی CCl4 و در نتیجه مهار فعالیت سیتوکروم  P450  و اثر خنثی کنندگی رادیکال‌های آزاد می‌باشد

کلیدواژه‌ها


عنوان مقاله [English]

The protective effects of marigold (Calendula officinalis) extract in liver damage by CCl4 in broiler chicken

نویسندگان [English]

  • S.، Moghaddam 1
  • H., Kermanshahi 2
  • R., Vahed 3
  • H., Nasiri Moghaddam 2
1 Ph.D. Student, Sari University of Agricultural and Natural Resources Sciences
2 Member of Scientific Board of Mashhad Ferdowsi University; Vahed, R., M.Sc., Mashhad Ferdowsi University
3 M.Sc., Mashhad Ferdowsi University
چکیده [English]

This experiment was conducted to determine the effects of Marigold Oil Extract (MOE) on blood parameters, immune system and livers of broiler chickens in a 42-day period. A total of 200 Ross 308 broiler chickens were allocated to five dietary treatments with four replicates of 10 birds each. The experimental treatments included a negative control group (basal diet  without using any MOE and carbon tetrachloride, CCl4), a positive control group}CCl4(1 mg/kg BW) and basal diet without using any MOE {and three levels of  MOE at 150, 300 and 450 mg/kg BW together with CCl4 and basal diet. Levels of blood parameters (SGOTو SGPT) increased, and MOE was able to reduce the negative effects of this toxic substance. When the broilers were 33 and 42 days old, the liver weights in treatments receiving various concentrations of MOE besides CCl4 were significantly lower than those in the treatment receiving CCl4. As for the indices related to immune system, the IgG level in the treatment receiving 300 mg/Kg of MOE in addition to CCl4 was significantly highercompard to the negative control and level of 150 mg/Kg of MOE . Examination of histopathological changes in the liver tissues revealed that the positive control group suffered the maximum tissue damage in the central vein, hepatocytes, sinusoids, and the triad port (the hepatic artery, the portal vein, and the bile ducts), while the negative control group exhibited the minimum tissue damage. However, treatments receiving MOE together with CCl4 showed greater recovery in the tissues compared to the positive control group. In conclusion,These results indicated the protective effects of MOE on the liver.  These effects are probably due to the ability of the flavonoids found in this plant to suppress the bioactivation of CCl4 and, hence, to suppress the activity of Cyt P450, and due to the neutralization of free radicals by the flavonoids.

کلیدواژه‌ها [English]

  • carbon tetrachloride
  • Marigold extract
  • Liver toxicity
  • Broiler Chickens
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