اثر حفاظتی گل همیشه بهار در بروز آسیب کبدی ناشی از تتراکلرید کربن در جوجه‌های گوشتی

نوع مقاله : مقاله کامل

نویسندگان

1 دانشجوی دکترای دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 عضو هیات علمی دانشگاه فردوسی مشهد

3 کارشناسی ارشد دانشگاه فردوسی مشهد

چکیده

این مطالعه به‌‌ منظور بررسی اثر سطوح مختلف عصاره‌‌ روغنی ‌‌گل‌‌ همیشه‌‌ بهار بر آنزیم های کبدی و تغییرات هیستوپاتولوژی بافت کبد جوجه‌‌های گوشتی طی یک دوره 42 روزه انجام گرفت.تعداد 200 قطعه جوجه‌‌ گوشتی نر سویه‌‌ راس 308 در یک طرح کاملاَ تصادفی با پنج گروه و چهار تکرار و 10 پرنده در هر تکرار مورد آزمایش قرار گرفتند. تیمارهای آزمایشی شامل گروه کنترل منفی (فاقد افزودنی عصاره‌‌ گل‌‌ همیشه‌‌ بهار و تتراکلریدکربن)، کنترل مثبت {تتراکلریدکربن (یک میلی‌‌گرم بر کیلوگرم وزن بدن) و فاقد افزودنی عصاره‌‌ گل‌‌ همیشه‌‌ بهار}و سه تیمار دیگر شامل سطوح 150، 300 و 450 میلی‌‌گرم در کیلوگرم جیره‌‌ عصاره گل همیشه-بهار به‌‌ همراه تتراکلریدکربن بود. فراسنجه‌‌های خون (SGOT و SGPT)،  که در اثر تزریق تتراکلریدکربن، سطح‌‌شان در خون افزایش یافته بود، عصاره گل همیشه بهار توانست اثرات منفی ناشی از این ماده سمی را تقلیل دهد. وزن نسبی کبد در تیمارهایی که علاوه بر تتراکلریدکربن، سطوح مختلف عصاره گل همیشه بهار را دریافت کرده بودند، نسبت به تیمار دریافت کننده‌‌ی تتراکلریدکربن، به‌‌صورت معنی‌‌داری پایین تر
بود (05/0>P.).در شاخص‌‌های مربوط به سنجش سیستم ایمنی، سطح ایمنوگلوبولین G در تیماری که علاوه بر تتراکلریدکربن، سطح 300 میلی‌‌گرم عصاره گل همیشه‌‌بهار را دریافت کرده بودند نسبت به گروه کنترل منفی و غلطت 150 همیشه بهار به‌‌صورت معنی‌‌داری بالاتر بود (05/0>P). در بررسی تغییرات هیستوپاتولوژی کبد، مشاهده شد که گروه کنترل مثبت از لحاظ وضعیت سیاهرگ مرکزی، هپاتوسیت‌‌ها، سینوزوئیدها و تریادپورت بیشترین میزان آسیب بافتی و گروه کنترل منفی کمترین میزان آسیب‌‌دیدگی را داشتند. اما تیمارهایی که همراه با تتراکلریدکربن، عصاره‌‌ی گل همیشه‌‌ بهار را دریافت کرده بودند در مقایسه با گروه کنترل مثبت، بهبود بیشتری در بافت مشاهده شد (05/0>P). این نتایج نشان دهنده اثر حفاظتی عصاره گل همیشه‌‌ بهار بر  کبد می باشد که احتمالاً بواسطه فلاونویید های موجود در گیاه و توانایی آنها در مهار فعال سازی زیستی CCl4 و در نتیجه مهار فعالیت سیتوکروم  P450  و اثر خنثی کنندگی رادیکال‌های آزاد می‌باشد

کلیدواژه‌ها

عنوان مقاله [English]

The protective effects of marigold (Calendula officinalis) extract in liver damage by CCl4 in broiler chicken

نویسندگان [English]

  • S.، Moghaddam 1
  • H., Kermanshahi 2
  • R., Vahed 3
  • H., Nasiri Moghaddam 2
1 Ph.D. Student, Sari University of Agricultural and Natural Resources Sciences
2 Member of Scientific Board of Mashhad Ferdowsi University; Vahed, R., M.Sc., Mashhad Ferdowsi University
3 M.Sc., Mashhad Ferdowsi University
چکیده [English]

This experiment was conducted to determine the effects of Marigold Oil Extract (MOE) on blood parameters, immune system and livers of broiler chickens in a 42-day period. A total of 200 Ross 308 broiler chickens were allocated to five dietary treatments with four replicates of 10 birds each. The experimental treatments included a negative control group (basal diet  without using any MOE and carbon tetrachloride, CCl4), a positive control group}CCl4(1 mg/kg BW) and basal diet without using any MOE {and three levels of  MOE at 150, 300 and 450 mg/kg BW together with CCl4 and basal diet. Levels of blood parameters (SGOTو SGPT) increased, and MOE was able to reduce the negative effects of this toxic substance. When the broilers were 33 and 42 days old, the liver weights in treatments receiving various concentrations of MOE besides CCl4 were significantly lower than those in the treatment receiving CCl4. As for the indices related to immune system, the IgG level in the treatment receiving 300 mg/Kg of MOE in addition to CCl4 was significantly highercompard to the negative control and level of 150 mg/Kg of MOE . Examination of histopathological changes in the liver tissues revealed that the positive control group suffered the maximum tissue damage in the central vein, hepatocytes, sinusoids, and the triad port (the hepatic artery, the portal vein, and the bile ducts), while the negative control group exhibited the minimum tissue damage. However, treatments receiving MOE together with CCl4 showed greater recovery in the tissues compared to the positive control group. In conclusion,These results indicated the protective effects of MOE on the liver.  These effects are probably due to the ability of the flavonoids found in this plant to suppress the bioactivation of CCl4 and, hence, to suppress the activity of Cyt P450, and due to the neutralization of free radicals by the flavonoids.

کلیدواژه‌ها [English]

  • carbon tetrachloride
  • Marigold extract
  • Liver toxicity
  • Broiler Chickens

مراجع

پیش بین، ش.، خوانساری، ن. و شایگان، م. 1381. بررسی ارتباط بین قدرت آنتی اکسیدانی تام پلاسما و دو عملکرد سیستم ایمنی (پاسخ تکثیری لنفوسیت­ها و حرکت هدف دار نوتروفیل ها). مجله دانشکده دامپزشکی دانشگاه علوم پزشکی تهران، شماره 5: صفحات 354 تا 363.
Abdollahi, M.R., Kamyab A, Bazzaz zadekan A, Nik-Khah,A  andZare ShahnehA.Z. 2002. Effect of different levels of Timus oil on broiler performance. Int J poult. Sci. 5(2): 156-161.
Adzet, T., Camarasa, J and Laguna, J.C. 1987.Hepatoprotective activity of polyphenolic compounds from Cynarascolymus against CCL4 toxicity in isolated rat hepatocytes. Natural. Pro., 50: 612-617.
Aniya, Y., Koyama, T., Miyagi, C., Miyahira, M., Inomata, C., Kinoshita, S. 2005. Free  radical scavenging and hepatoprotective actions of medicinal herbs, Crassocephalum crepidioides from the Okinawa Islands. Bio. Pharm. Bull.28: 19-23.
Avigen. 2009. Ross 308 broiler manual. http://en.aviagen.com/ross-308/.
Azzaz, N.A., Hassan, E. A., Emarey, F. A. 2009. Physiological, anatomical, and biochemical studies on pot marigold (Calendula officinalis L.) plants. African. Crop. Sci. 8:1727-1738.
Baer-Dubowska, W., Szaefer, H. and Krajka- Kuzniak, V. 1998.Inhibition of murin hepatic cytochrom P450 activities by natural and synthetic phenolic compounds.Xenobiotica. 28:735-743.
Basavaraj, V.C., Karnakumar, V.B.and Rajabhau, S.Sh. 2011. Evaluation of
Hepatoprotective Activity of Flowers of “Tagetes erecta linn”.  Int. J. Pharm&Bio.2:692-695.
Basch, E., Bent, S., Foppa, I., Haskmi, S., Krol, D., Mele, M., Szapary, P., Ulbricht, C., Vora, M. and Yong, S. 2006. Marigold (Calendula officinalis L.): An evidence-based systematic  reviewby the natural standard research collaboration, J. Herb. Pharmacother, 6(3-4) 135.
Boll, M., Weber, L. W. D., Becker, E. and Stampfi, A. 2001. Mechanism of carbon
tetrachloride induced hepatotoxicity. Hepato-cellular damage by reactive CCL4 metabolites
 Zei. Nat. J. 56: 649-659.
Brenes, A and Roura, E. 2010. Essential oils in poultry nutrition: main effects and modes of  action. Anim. Feed Sci. &Tech. 158: 1-14.
Chalchat, J.C., Garry, R. P., Michet, A. 1991. Chemical composition of essential oil of
Calendula officinalis L. (pot marigold).Flav. & Frag. J. 6(3): 189–192.
Chinnasamy, S., Murugan, R., Rathinasamy, P., Ganesan, K. 2011. Hepatoprotective Effect of  Herbitars, A Polyherbal Formulation Against CCL4 Induced Hepatotoxicity in Rats. J. Pharm. Res. 4(3): 676-679.
Christake, E., Paneri, v., Giannenas, I., Papazahariadou, M., Botsoglou, N.A  and. Spais, A.B. 2004. Effect of mixture of herbal extract on broiler chicken infected with eimeria tenella. Anim. Res. 53: 137-144.
Cordova, C.A., Siqueira, I.R., Netto, C.A., Yunes, R.A., Volpato, A. 2002.Protective  properties of butanolic extract of the Calendula officinalis L. against lipid peroxidation of rat liver microsomes and action as free radical scavenger. Redox. Rep. 7: 95-102.
Drotman, R.B. and Lawhorn, G.T., 1978. Serum enzymes as indicators of chemical-induced liver damage.Drug & Chem. Toxi.1: 163–171.
Duncan, D.B. 1955. Multiple range and multiple F tests, Biometrics. 11: 1 – 42
Fernandez, G., Villarruel, M. C., De Ferreyra, E. C., De Fenos, O., Mbernacchi, A. S., De  Castro, C. R and Castro, J. A. 1984. Carbon tetrachloride-induced early biochemical  alterations but not necrosis in pigeon's liver. Inf. Res. 15: 463-466.
Ferre, N., Camps, K., Cabre, M., Paul, A. 2001.Hepatic paraoxygenase activity alterations and free radical production in rats with experimental cirrhosis.Met. J. 50: 997-1000.
Fronza, M., Heinzmann, B., Hamburger, M., Laufer, S., Merfort, I. 2009. Determination of the  wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J. Ethn. 126(3): 463-7.
Fossati P and Lorenz P. 1982 Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. J. Clin. Chem. 28: 2007
Guinot, P., Gargadennec, A., Valette, G., Fruchier, A., Andary, C. 2008. Primary flavonoids in marigold dye: extraction, structure and involvement in the dyeing process. Phyt. Annal. 19(1): 46-51.
Harsh, M. 2005.Text book of Pathology. 5Th. New Delhi; Jaypee Brothers. 608-10.
Jayasekhar, P., Mohanan, P. V., Rathinam, K. 1997. Hepatoprotective activity of ethyl acetate extract of Acacia catechu.Ind. J. Pharm. 29: 426-8.
Havel, R. J. 1986.Functional activities of hepatic lipoproteins receptors.Annu. Rev. Phys. 48: 119-134.
Hernandez, F., J. Madrid, V. Garcia, J. Orengo and M. D. Megias. 2004. Influence of two plant extracts on broiler performance, digestibility and digestive organ size. Poult. Sci. 83: 169-174.
Jayasekhar P, Mohanan PV, Rathinam K .1997. Hepatoprotective activity of ethyl acetate extract of Acacia catechu.Indian JournalPharmaceutical.29: 426-428.
Jordan FTW, Pattison M .1992.Poultry  disease 4th edition. W.B.Sauder company ltd london. 169-171.
Kassab, S., Cummings, M., Berkovitz, S., van, H. R., Fisher, P. 2009. Homeopathic medicines for adverse effects of cancer treatments. Coch. Data. Syst. Rev. 15(2): CD004845.
Kasprzyk, Z., Pyrek, J. 1968. Triterpenic alcohols of Calendula officinalis L. flowers Phytochemistry. J. Anim. Sci. 7(9): 1631-1639.
Kumar, S. S., Kumar, B. R. and Mohan, G. K. 2009. Hepatoprotective effect of  TrichosanthescucumerinaVarcucumerina L. on carbontetrachloride induced liver damage in rats. J. Ethn.123: 347–350.
Lin, L.T., Liu, L.T., Chiang, L.C., Lin, C.C. 2002.In vitro anti-hepatoma activity of fifteen natural medicines from Canada. Phyto. Res. 16: 440-444.
Meyer, S.A. and Kulkarni, A.P. 2001. Hepatotoxicity. In: Introduction to biochemical  toxicology. New York. John. 487.
Muley,  B.P., Khadabadi, S.S., Banarase,  N.B. 2009. Phytochemical constituents and pharmacological activities of Calendula officinalis L. Rev. Trop. J. Pharm. Res.8: 455-465.
Pietta, P., Bruno, A.M.P., Rava, A. 1992. Separation of flavonol-2-O-glycosides from  Calendula officinalis and Sambucus nigra by high performance liquid and micellar  electrokinetic capillary chromatography. J. Chrom.593: 165-170.
Preethi, K.C and Kuttan, G. 2009. Antiinflammatory activity of flower extract of Calendula  officinalis Linn. And its possible mechanism of action. Ind. J. Exp. Bio.47: 113-20.
Pyo, Y.H., Lee, T.C., Logendra, L. and Rosen, R.T. 2004. Antioxidant activity and phenoliccompounds of Swiss chard (Beta vulgaris subspecies cycla) extracts.Food Chemistry. 85,19-26.
Reitman S and Frankel S. 1957.A Colorimetric method for the determination of serum glutamic oxaloacetic transminase and serum glutamic pyruvic transaminase.American. J . Clin. Path. 20: 56-59.
Ríos, J.L., Recio, M.C., Máñez, S., Giner, R.M. 2000. Natural triterpenoids as anti inflammatory agents. Studies in Natural Products Chemistry.22(3): 93- 143.
Robjohns, S. 2009. Carbon tetrachloride toxicological overview.J. Health Pro. Agen. 1-11.
Rusu, M. A., Tamas, M., Puica, C. 2005. Ioana Roman and Mihaela Sabadas. TheHepatoprotective Action of Ten Herbal Extracts in CCL4 Intoxicated Liver.Phyt. Res. 19:  744–749.
Sadeghi, H. Ghaitasi, E. Mazrooghi, N. Sabzali, S. 2007. The hepatoprotective effects of dorema auchri on carbon tetrachloride induced liver damage in rats. J shahrekord univ med sci. 9: 38-43
Saman, S. and Cook, N.C. 1996.Flavonoids chemistry, metabolism, cardio protective effects, dietary sources.J. Nut. Bio.7: 66-76.
Sadeghi H, Ghaitasi E, Mazrooghi N, Sabzali S .2007. The hepatoprotective effects of dorema auchri on carbon tetrachloride induced liver damage in rats. J shahrekord university of medical science journal. 9: 38-43.
SAS (Statistical Analysis System). 2001. SAS/STAT® 8.0. User's guide. SAS Institute Inc. Cary, NC.
Satoshi, M., Kiyoto, S., Hiroe, M., Makoto, I., Takenori, Y., Torao, I. and Yeunhwa, G.U.2004.Antioxidant and immune enhancing of Echinacea purpurea. Biol Pharmaceut Bull. 27: 1004-1009.
Sell, S. 1990. Is there a liver stem cell.Cancer Res. 50: 3811.
Sethuraman, M. G., Lalitha, K.G., Rajkapoor, B. 2003. Hepatoprotective activity of  Sarcostemma brevistigma against carbon tetrachloride-induced hepatic damage in rats. Curr. Sci. 84: 1186-7.
Schuppan, D., Jia, J. D., Brinkhaus, B. and Hahn, E. G. 1999. Herbal products for liver diseases.J. Hepatology. 30(4): 1099-1104.
Shams Ghafarokhi, M., Razafsha, M.,Allameh, A. and Razzaghi Abyaneh, M. 2003. Inhibitory effect of Aqueous Onion and Garlic Extracts on growth and keratinase activity in trichophyon Mentagro phytes.J. Irn. Biomed. 7: 113-118.
Shimizu, H., Uetsuka, K., Nakayama, H. and Doi, K. 2001. carbontetrachloride-induced liver acute liver injury in Mini and Wister rats. J. Exp. & Tox. Path. 53(1): 11-17.
Sun, F.. 2000. Evaluation of oxidative stress based on lipid hydroperoxide, vitamin C and vitamin E during apoptosis and necrosis caused by thioacetamide in rat liver. Biochimica et Biophysica Acta ,1500: 181-185.
Takahashi, K., Mashiko, T. and Akiba, Y. 2000. Effects of dietary concentration of xylitol on
growth in male broiler chicks during immunological stress. Poult. Sci. 79: 743-747.
Ulicna, O., Greksak, M., Vancova, O., Zlatos, L., Galbavy, S. and Bozek, P. 2003.  Hepatoprotective effect of Roobos tea (Asppalathuslinearis) on CCL4 induced liver damage in rats. J. Phys. Res. 52: 461-466.
Venukumar, M.R., Latha, M.S. 2002. Hepatoprotective of the methanolic extract of  Curculigo orchioides in CCL4-treated male rats. Ind. J. Pharm. 34: 269-75.
Wilkinson JH, Baron DN, Moss DW, Wolter PG. 1972. Standardization of clinical enzyme assays: Reference method for aspartate and alanine transaminase. J. Clin. Path. 25: 940.
Wegmann, T. and Smithies, O. 1966. A simple hemagglutination system requiring small amounts of red cells and antibodies.J.Transfusion. 6:67-75.
Wells, F. E. 1988.Tests in liver and biliary tract disease. In: Gowenlock, H. A. (Ed.), Varley’s Pract. Clin. Bio. CRC Press, Florida.
Wilkinson JH, Baron DN, Moss DW, Wolter PG. 1972. Standardization of clinical enzyme assays: Reference method for aspartate and alanine transaminase. J.Clin. Path. 25: 940.
Wolf, P. L. 1999.Biochemical diagnosis of liver disease.J. Clin. Bio. 14: 59-64.
Yang, H., Lee, M.K., Kim, Y.C. 2005.Protective activities of stilbene glycosides from Acermono leaves against H2O2-induced oxidative damage in primary cultured rat hepatocytes. J. Agri. Food Chem. 53: 4182-6.
Zaragoza, A., Andres, D., Sarrion, D. and Cascales, M. 2000.Potentiation of thioacetamide hepatotoxicity by Phenobarbital pretreatment in rats, inducibility of FAD monooxygenase system and age effect. Chemico-Biological Interactions ,124: 87-101.
Zix, M.H. and Agarwal, R. 1997. Novel cancer chemopreventive effects of a flavonoid antioxidant silymarin.Biochm. Biophys. Res. Commun., 239: 334-339.
Zlatkis A, Zak B, and Boyle AJ. 1953. A new method for the direct determination of serum cholesterol. J.Clin. Med. 41: 486-492.

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  • تاریخ دریافت: 01 مهر 1393
  • تاریخ بازنگری: 01 آذر 1394
  • تاریخ پذیرش: 01 اسفند 1393

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