عنوان مقاله [English]
Bovine Theileriosis have been producing in Iran nearly four decades. While Bovine Theileriosis vaccine administration is now very crucial for disease control, therefore it is important to improve the quantity and quality of the vaccine for providing the national and export markets. The growth of Theileria annulata infected cell line S15 vaccine strain in Stoker cell culture medium including 10% adult bovine serum in wolf simple cell culture vessel, Biomixer and in "Labfors" fully controlled aerobic bioreactor fed-batch system has revealed the efficient growth of T. annulata infected cell line in all employed vessels. The obtained results determined the 250% cell growth in "Wolf" vessel, 180% in Labfors aerobic bioreactor and finally 200% for Biomixer vessel. The results for quality control of produced vaccine in proposed technique have shown the good quality of the prepared bovine Theileriosis vaccine in aerobic bioreactor system. In conclusion, according to the achieved findings, the production of bovine Theileriosis vaccine in aerobic bioreactor system might be used for high production and less manipulation of production processes as well as saving labor in order to mechanizing the production and better controlling the process of manufacturing.