بررسی اثر کاشت ملاتونین بر عملکرد غده فوق کلیه میش نژاد عربی در خارج از فصل تولیدمثلی

نوع مقاله : مقاله کامل

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی دام دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

2 دانشیار گروه علوم دامی دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

3 استادیار گروه علوم دامی دانشگاه کشاورزی و منابع طبیعی رامین خوزستان

4 استاد دانشکده دامپزشکی دانشگاه شهید چمران اهواز

چکیده

هدف از این تحقیق، بررسی اثر ایمپلنت ملاتونین بر عملکرد غده فوق کلیه و میزان هورمون کورتیزول سرم خون در خارج از فصل تولیدمثلی میش‌های نژاد عربی بود. بدین منظور 16 رأس میش عربی 2 تا 4 ساله (با میانگین وزن 5±35 کیلوگرم) غیرآبستن به دو گروه آزمایشی تقسیم شدند. این تحقیق از اوایل دی ماه تا اواسط اسفند که مصادف با فصل غیرتولیدمثلی میش عربی می‌باشد، انجام شد و طول دوره‌ی آزمایش 10 هفته بود. در گروه اول (شاهد) هیچ گونه درمانی صورت نگرفت. در گروه دوم از کاشت ملاتونین استفاده شد. وزن غده فوق کلیه و ضخامت نواحی قشر، کپسول، گلومرولوزا، فاسیکولاتا و رتیکولاریس این غده در تیمار کاشت ملاتونین نسبت به تیمار شاهد به طور معنی‌داری کاهش یافت (01/0 > p). اختلاف آماری معنی‌داری در میزان کورتیزول سرم خون بین دو تیمار شاهد و کاشت ملاتونین در تمامی زمان‌های مورد مطالعه وجود نداشت (05/0 < p). در گروه شاهد، میزان هورمون کورتیزول سرم خون در روز 14 پس از کاشت ملاتونین بطور معنی‌داری بیشتر از روزهای 42 و 56 بود (01/0 > p). اما در گروه تیمار بین زمان‌های مختلف تفاوت معنی‌داری مشاهده نشد  (05/0 < p). طبق نتایج حاصل، استفاده از ملاتونین جهت القاء فصل تولیدمثلی در میش عربی، یک عامل استرس زا محسوب نمی‌شود و کاربرد آن موجب افزایش میزان کورتیزول نمی گردد

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of melatonin implant on adrenal gland function in Arabian ewe in out of breeding season

نویسندگان [English]

  • Kianfar, M. 1
  • M. Mamouei, 2
  • S. Tabatabaei-Vakili, 3
  • Kh. Mirzadeh, 3
  • N. Erfani-Majd, 4
1 M.Sc. student of Animal Physiology, Ramin Agriculture and Natural Resources University of Khuzestan.
2 Associated Professor, Department of Animal Sience, Ramin Agriculture and Natural Resources University of Khuzestan.
3 Assistant Professors, Department of Animal Sience, Ramin Agriculture and Natural Resources University of Khuzestan.
4 Professor, Faculty of Veterinary Medicine, Shahid Chamran University of Ahwaz.
چکیده [English]

The purpose of this study was to investigate the effect of  melatonin implant on adrenal function and serum cortisol level in Arabian ewes out of the breeding season. For this purpose, 16 Arabian ewes with 2 to 4 years old (mean weight 35±5 kg) and non-pregnant were divided into two groups. The survey was conducted from late December to early March for 10 weeks. The first group (control) received no melatonin. Implantation of melatonin was used in the second group. Weight of adrenal gland in treatment of melatonin implant in compare with control group is significantly dicrease (p<0.01). Weight of adrenal gland and thickness of the cortex, capsule, glomerulosa, fasciculate and reticularis in treatment of melatonin implant has been decreased in compare with control group (p<0.01). In all times of study, there was no significant differences in cortisol levels between melatonin and control treatments (P>0.05). in control group, the cortisol concentration of blood serum in day 14 was significantly higher than days 42 and 56 (p<0.01). But in melatonin treatment, there were no significant different in cortisol value among all times of study (P>0.05). With regard to the results of this study can be said, use of melatonin to induction breeding season in Arabian ewes, not as a stressor and its application in out of the breeding season did not increase the cortisol level. 

کلیدواژه‌ها [English]

  • Adrenal gland
  • Arabian ewe
  • Cortisol
  • Melatonin
  • Serum
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