نوع مقاله : مقاله کامل
نویسندگان
1 گروه ریاضی،دانشکده علوم، دانشگاه زنجان، ایران
2 گروه بیوتکنولوژی، پژوهشکده فناوریهای نوین زیستی دانشگاه زنجان، دانشگاه زنجان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Introduction: Brucella ovis is a prevalent infectious disease in sheep manifesting in clinical and subclinical forms. This pathogen causes reproductive tract lesions, reduced fertility, abortion, and increased perinatal mortality in lambs. Brucella ovis-induced epididymitis generates pathological alterations in the epididymis, testes, and accessory sex glands, leading to impaired ram fertility. These alterations may compromise the utility of ram sperm for in vitro fertilization (IVF), resulting in reduced fertilization rates. Furthermore, poor sperm quality may impair sperm survival during freeze-thaw procedures.
Objective: This study aims to analyze gene expression profiles in Brucella ovis-infected sheep to identify key genes and associated biological pathways linked to this infection.
Methods: The dataset used in this study was extracted from the GEO database (Accession Number: GSE35615). Samples were collected from six distinct glands (epididymis, vas deferens ampullae, vesicular glands, bulbourethral glands, testes, and a composite of inguinal and scrotal lymph nodes) at three infection stages: acute phase (60 dpi), chronic phase I (120 dpi), and chronic phase II (240 dpi), alongside healthy controls (0 dpi). Gene expression profiling was performed using the Affymetrix Bovine Genome Array. Comparative analysis of gene expression levels across these six glands at different infection stages was conducted.
Findings: Through rigorous bioinformatic and statistical analyses, 45 genes exhibiting significant differential expression with high regulatory significance were identified. These genes play pivotal roles in molecular binding, protein binding, molecular function, and cellular component pathways.
Conclusion: This study provides valuable insights into the molecular mechanisms underlying Brucella ovis infection and its impact on in vitro fertilization. The findings present potential targets for further research in veterinary biotechnology and reproductive health management.
کلیدواژهها [English]
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