نوع مقاله : مقاله کامل
نویسنده
پژوهشکده کشاورزی، سازمان پژوهشهای علمی و صنعتی ایران، تهران، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسنده [English]
Introduction: Screening of poultry products is crucial to prevent antibiotic residues from entering into the human food chain and maintaining public health. Thermophilic bacteria from the genera Geobacillus and Bacillus rank the most well-known indicator microorganisms in test tube- based microbial inhibition assays for screening animal products.
Objective: The current study was carried out to asses the antibiotic susceptibility of Bacillus licheniformis (BL) PTCC 1721 and Geobacillus stearothermophilus (GS) IBRC-M 10771 obtained from local microbial collections to different permitted or banned antibiotics for use in food-producing poultry.
Methods: The minimum inhibitory concentration (MIC) of enrofloxacin, sulfamethazine and oxytetracycline as commonly utilized poultry antibiotic class types, was assessed by broth microdilution method. Moreover, antibiotic susceptibility to 12 antibiotics was evaluated using the disk diffusion test.
Results: The results showed that MIC of enrofloxacin, sulfamethazine, and oxytetracycline for GS were 0.015, 0.003, and 0.062 mg/L, respectively. Likewise, the values for BL were 2, 1, and 0.125 mg/L, respectively. Compared to the standard values, these findings indicated that both strains were fully sensitive to the above antibiotics. The results from disk diffusion method additionally revealed that GS exhibited sensitivity to all the evaluated antibiotics. Conversely, BL demonstrated resistance to two antibiotics colistin and lincomycin, yet it was entirely sensitive to the other antibiotics assessed.
Conclusions: Based on the findings, both evaluated strains exhibited a good antibiotic sensitivity profile for applying as an indicator microorganism in microbial inhibition assays. However, the spectrum of sensitivity and hence potential for residues detection was found to be broader in GS compared to BL. Further studies are needed to determine the detection limit of the strains according to the maximum residue limits in poultry various tissues.
کلیدواژهها [English]
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