نوع مقاله : مقاله کامل
نویسندگان
1 گروه علوم پایه، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
2 گروه میکروبیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی آجا، تهران، ایران
3 مرکز تحقیقات نانوبیوتکنولوژی، دانشگاه علوم پزشکی بقیه اله، تهران، ایران
4 مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی مشهد، مشهد، ایران
5 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background: Active plant compounds, such as curcumin, are increasingly being used to treat cancer because of their anti-cancer properties and lower side effects. To further evaluate the potential of curcumin-loaded niosomal nanoparticles (CM-NP) as a drug delivery system, this study investigated the effect on cell survival and migration, as well as DNA damage, in the resistant lung cancer cell line A549 in comparison to the effect of free curcumin (CM). Methods: The A549 cells were treated with CM and CM-NP and evaluated by MTT, DNA fragmentation and cell migration methods. To determine the IC50 of the drugs as well as cell proliferation and survival, the cells were exposed to different concentrations of CM and CM-NP for 24, 48 and 72 hours. To assess DNA fragmentation, cells were stained with an acridine-orange solution. The cells were then observed and counted under a fluorescence microscope. To evaluate the inhibitory effects of CM and CM-NP on the migration of A549 cells, a wound healing migration model was used. Results: Treatment with both CM and CM-NP significantly decreased A549 cell proliferation in a time-dependent manner (P<0.05). The IC50s values of CM-NP and CM in the A549 cells were 107.2 and 39.48 µg/mL, respectively, after 24 hours. The IC50 of CM-NP significantly increased in the A549 cells with fragmented DNA compared to those in the free CM and control groups after 24 hours (P<0.01). Migration of the A549 cell was significantly lower in the CM-NP group than in the CM group, and this difference was influenced by both dose and duration of treatment (P<0.01). Conclusions: The results of the present study indicate that curcumin nanonaniosomes can be used as efficient carriers for the delivery of curcumin for the medical treatment of lung cancer.
کلیدواژه‌ها [English]
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