نوع مقاله : مقاله کامل
نویسندگان
1 علوم دامی- دانشکده کشاورزی- دانشگاه فردوسی مشهد- مشهد-ایران
2 علوم دامی- دانشکده کشاورزی- دانشگاه فردوسی مشهد- مشهد- ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Introduction: The development of digestibility plays an important role on improvement of feed efficiency. Bacteria produce beneficial enzymes that help to digest food. The use of commensal bacteria were evaluated for increase the digestion and absorption of feed nutrients in this study.
Materials and methods: Enzyme activity of 46 bacterial strains was tested by well method on solid medium containing a substrate of enzyme. These bacteria were isolated form digestive tract of broiler chicken and rumen of dairy cattle. The bacteria with the highest enzyme activity were isolated and examined on feed digestibility under different treatments. Food digestibility was measured by collecting the whole feces in Arian breed chickens.
Findings: The findings showed that rumen bacteria effectively secrete amylase, protease, cellulase, phytase and pectinase enzymes. On the contrary, Lactobacillus bacteria could not produce cellulase and pectinase enzymes but some of them effectively exhibited lipase and xylanase activity that formed different bacterial consortiums in 9 treatments. In feed digestibility experiment, composition of isolates significantly affected nutrient digestibility in broiler chickens. Treatment 6 showed the highest digestibility for dry matter and starch and suitable lipase activity.
Conclusion: According to the results of this experiment, Lactobacillus bacteria exhibited the ability to produce lipase and xylanase enzymes and, bacteria isolated from Holstein's digestive system showed the ability to produce cellulose and pectinase enzymes. In addition, bacterial consortiums effectively break down the substrate in the feed by extraction of amylase, phytase, cellulase and lipase enzymes, which leads to improved nutrient digestibility. Based of comparison of treatments, treatment of 5 and 6 displayed the best consortiums among other treatments and suggested to performance test in the farm.
کلیدواژهها [English]
)