نوع مقاله : مقاله کامل
نویسندگان
1 دپارتمان میکروب شناسی، دانشکده علوم پایه، واحد قم،دانشگاه آزاد اسلامی، قم، ایران کدپستی: 3749113191
2 موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، ترویج و آموزش کشاورزی، بخش تحقیق و تولید توبرکولین و مالئین، کرج ، ایران کدپستی:
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The economically-challenging paratuberculosis caused by Mycobacterium avium subspecies paratuberculosis (Map), is a contagious chronic disease that essentially affects ruminants. It bears characteristic clinical symptoms including incurable diarrhea, mesenteric lymphadenitis, proliferative enteropathy and progressive emaciation. Considering the evolutionary important variability of Map genome, characterization of its strains is pivotal in diagnosis and epidemiological investigation, and therefore establishing prevention and disease control strategies. The present work was therefore designed to extend our currently limited knowledge on population structure of Map in Iran. A total of 30 recently-archived Map isolates obtained from bovine, ovine and caprine hosts representing a diverse geographical origin were revived and sub-cultured to obtain the required genomic material. They were subsequently analyzed for the presence of IS900, the Map specific sequence, as well as IS1311 using PCR protocols. They were further typed using IS900 and IS1311 polymerase chain reaction-restriction endonuclease analysis (PCR-REA) using Alu I and Hinf I enzymes.
All the DNA samples were found positive for the two genetic markers of IS900 and IS1311. On top of this both IS900 and IS1311 PCR-REA analyses jointly demonstrated that showed that all Map strains under examination belonged to the cattle type.
Taking into account the diverse geography of ruminant exporting countries to Iran plus lacking reports of paratuberculosis in the indigenous ruminant breeds, the presence and circulation of the "cattle type" Map strains in the today’s Iran looks odd and deserves more investigation as this can have important epidemiological implications in regard with the paratuberculosis control and prevention in Iran.
کلیدواژه‌ها [English]
)