مطالعه بیوانفورماتیکی پروتئین مولتی اپی‌توپ طراحی شده بر اساس مناطق بسیار محافظت شده‌ پروتئین هماگلوتینین سویه‌های H9N2 و H5N8 ویروس آنفولانزا

نوع مقاله : مقاله کامل

نویسندگان

1 گروه میکروبیولوژی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

2 بخش بیوتکنولوژی، موسسه تحقیقات سرم سازی رازی، سازمان واکسن و ترویج کشاورزی، کرج، تحقیقات، آموزش و ایران

چکیده

واکسیناسیون علیه آنفلوانزای پرندگانH9N2/H5N8   به عنوان یک راهکار موثر برای مقابله و کنترل این پاتوژن های کشنده مطرح می باشد. به همین منظور در این مطالعه یک واکسن کایمریک دوگانه موثر علیه H9/H5 از ناحیه HA2 دوسویه H9N2 و H5N8 طراحی شد و با استفاده از مدل های بیوانفورماتیک، خصوصیات فیزیکوشیمیایی و تجزیه و تحلیل ساختار آنتی ژنیک اپی توپ های انتخاب شده مورد تجزیه و تحلیل قرار گرفت. برای انجام این مطالعه سویه های (A/chicken/Iran/B308B/2004)  H9N2 و (A/Poultry/Iran/clade 2344/2018)  H5N8 انتخاب شدند و اپی توپ های محرک لنفوسیت های B  و T بررسی و مناسب ترین آنها انتخاب شدند. سپس یک واکسن کایمریک با ترکیب اپی توپهای بسیار ایمنی زا طراحی شد. در ادامه با استفاده از نرم افزارهای مرتبط، ویژگیهای فیزیکوشیمیایی و ساختار ثانویه واکسن طراحی شده از لحاظ پایداری حرارتی و آبدوستی و  pH ایزوالکتریک و میزان آنتی ژنسیتی،آلرژنسیتی نیز مورد ارزیابی قرار گرفت. در نهایت واکسن پیشنهادی رونویسی معکوس شد و به منظور قرارگیری در وکتور بیانی پروکاریوتی PET 41 a(+) تطبیق داده شد. با توجه به نتایج حاصل، پیش بینی میشود که ساختارآنتی ژنیک طراحی شده در این مطالعه می تواند با موفقیت و به میزان قابل قبولی در سیستم های پروکاریوتی بیان شود و جهت مطالعات ایمنی زایی علیه سویه های H5N8 وH9N2  ویروس آنفلوآنزا مورد استفاده قرار گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Bioinformatic Anaysis of Multi-epitope Protein Designed Based on Highly Conserved Regions of Hemagglutinin Protein of H9N2 and H5N8 Influenza Virus Strains

نویسندگان [English]

  • zahra Bozorgkhoo 1
  • Behzad Hemmati 1
  • Morteza Taghizadeh 2
  • Majid Tebianian 2
  • Mostafa Ghaderi 1
1 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2 Department of Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده [English]

Vaccination against bird flu H9N2/H5N8 is considered as an effective strategy to deal with and control these deadly pathogens. For this purpose, in this study, an effective dual chimeric vaccine against H9/H5 from the bidirectional HA2 region of H9N2 and H5N8 was designed and analyzed using bioinformatics models, physicochemical characteristics and antigenic structure analysis of selected epitopes.To carry out this study, (A/chicken/Iran/B308B/2004) H9N2 and (A/Poultry/Iran/clade 2344/2018) H5N8 strains were selected and the epitopes stimulating B and T lymphocytes were investigated and the most suitable ones were selected. Then a chimeric vaccine was designed by combining highly immunogenic epitopes. In the following, using related software, the physicochemical characteristics and secondary structure of the designed vaccine were evaluated in terms of thermal stability, hydrophilicity, isoelectric pH, antigenicity and allergenicity. Finally, the proposed vaccine was reverse transcribed and adapted to be placed in the prokaryotic expression vector pET-41 a(+). According to the results, it is predicted that the antigenic structure designed in this study can be expressed successfully in prokaryotic systems and use for immunogenic studies against H5N8 and H9N2 strains of influenza virus.    
                     

کلیدواژه‌ها [English]

  • influenza virus
  • multi-epitope
  • hemagglutinin
  • bioinformatics

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  • تاریخ دریافت: 30 آذر 1401
  • تاریخ پذیرش: 03 دی 1401

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