بررسی وضعیت سلنیوم خون گله‌های گاو شیری در گاوداری‌های صنعتی استان اصفهان

نوع مقاله : مقاله کامل

نویسندگان

1 بخش تحقیقات علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اصفهان؛ ایران

2 موسسه تحقیقات واکسن و سرمسازی رازی، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران

چکیده

سلنیوم یکی از عناصر ضروری برای گاو شیری به ویژه برای تولید مثل آن محسوب می شود. این مطالعه به منظور تعیین میزان سلنیوم خون گروه‌های مختلف گاوشیری در گاوداری‌های صنعتی استان اصفهان و همچنین اثر فصل(تابستان و زمستان) بر غلظت آن در قالب طرح بلوک کامل تصادفی انجام شد. بدین منظور 553 رأس گاو از 17 واحد گاوداری صنعتی در نقاط مختلف جغرافیایی استان انتخاب گردید. جمعیت هر گاوداری به 5 گروه مختلف شامل گاوهای پرتولید (154 رأس)، گاوهای تازه‌زا (118 رأس)، خشک و تلیسه آبستن (121رأس)، تلیسه‌های غیرآبستن (81 رأس) و گوساله‌ها (79 رأس) تقسیم شدند. میزان سلنیوم خون به وسیله دستگاه اتو آنالایزر آلفا کلاسیک به روش غیرمستقیم و با اندازه‌گیری فعالیت آنزیم گلوتاتیون پراکسیداز تعیین گردید. براساس نتایج، میانگین غلظت سلنیوم خون در گاوهای پرتولید  برابر 21/0 میلی‌گرم بر لیتر، گاوهای تازه‌زا 18/0 میلی‌گرم بر لیتر، خشک و تلیسه آبستن 24/0 میلی‌گرم بر لیتر، تلیسه‌های غیرآبستن 24/0 میلی‌گرم بر لیتر و گوساله‌ها 21/0 میلی‌گرم بر لیتر بدست آمد. گاوهای خشک و تلیسه آبستن و همچنین تلیسه‌های غیرآبستن با بیشترین میزان سلنیوم خون نسبت به گاوهای پرتولید، تازه‌زا و گوساله‌ها تفاوت معنی‌داری نشان دادند (05/0>P). کمترین میزان سلنیوم خون نیز به گاوهای تازه‌زا تعلق داشت که با سایر گروه‌ها تفاوت معنی‌‌داری نشان داد (05/0>P). بین گروه‌های مختلف گاو شیری از نظر میزان فعالیت آنزیم پراکسیداز تفاوت معنی‌داری مشاهده نشد. میانگین غلظت سلنیوم خون در تابستان (129 رأس) 0.08± 0.21   میلی‌گرم بر لیتر و در زمستان (174 رأس) 0.06±0.20  میلی‌گرم بر لیتر بود که نشان‌دهنده عدم تاثیرمعنی‌دار فصل بر میزان سلنیوم خون بود. همچنین، مقایسه هر گروه گاوشیری با هم گروه خود در گاوداری‌های مختلف نیز وجود تفاوت معنی‌دار از نظر میزان سلنیوم خون را به اثبات رسانید (05/0>P). توصیه می‌شود، ابتدا میزان سلنیوم خون گله بررسی شود و سپس مکمل سلنیوم زیر نظر متخصص تغذیه دام مورد استفاده قرار گیرد. 

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of Blood Selenium Status of Dairy Cattle Herds in Isfahan Province Industrial Cattle Farms

نویسندگان [English]

  • Sayyed Kamaleddin Allameh 1
  • Mohsen Rasti 1
  • Ahmad Reza Ranjbari 1
  • Vahid Noaman 2
  • Masood Boroumand 1
1 Animal Science Research Department, Isfahan Agriculture and Natural Resources Research and Education Center; Agriculture Research, Education and Extension Organization (AREEO), Isfahan, Iran.
2 Razi Vaccine and Serum Research Institute, Agricultural Research , Education and Extension Organization, (AREEO), Karadj, Iran.
چکیده [English]

Selenium is an essential element in dairy cattle, particulrarly for reproduction. The present study was conducted in order to determine the amount of selenium in the blood of different groups of cattle in Isfahan province industrial cattle farms and also the effect of season (summer and winter) on its concentration in the form of a randomized complete block design. The present study was investigated the blood selenium level in dairy cattle in Isfahan province. Also, the effect of season (summer and winter) on selenium values was studied. This experiment was carried out in a complete block randomized design for 553 cows in 17 farms from different regions of the Isfahan province. Acordingly, 553 cows were selected from 17 industrial cattle breeding units in different geographical parts of the province. The population of cows in each cattle farm wasere divided into five groups which included high producing cows (n=154), fresh cows (n=118), dry and pregnant heifers (n=121), non-pregnant heifers (n=81) and calves. Blood selenium concentration was indirectly assessed determined through the measurement of glutathione peroxidase activity by Alpha Classic Auto Analyzer. According to the results, the mean whole blood selenium concentration mean for high producing cows, fresh cows, dry and pregnant heifers, non-pregnant heifers and calves were measured at 0.21, 0.18, 0.24, 0.24, 0.21 mg/L, respectively. Dry and pregnant heifers and non-pregnant heifers with higher selenium concentration showed significant differences compared to other groups (P<0.05). The lowest selenium concentration was significantly observed for fresh cows (P<0.05). No significant differences were found among different groups of dairy cows in terms of peroxidase enzyme activity for glutathione peroxidase activity. The mean blood selenium concentrations in summer and winter with no significant differences were at 0.21± 0.08  (n=129) and 0.20±0.06  (n=174) mg/L, respectively which showed no significant effect of season on blood selenium level. Also, the comparison of the same group in different farms showed significant difference in terms of blood selenium levels s between them (P<0.05).Therefore, it is recommended to check the blood selenium level of dairy cattle and then ,use a selenium supplement under supervision of animal nutritionists.

کلیدواژه‌ها [English]

  • Dairy Cattle
  • Industrial
  • Supplement
  • Blood
  • Selenium
  • Glutathione Peroxidase
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