بررسی تنوع ژنوتیپی سویه‌های باکتری Paenibacillus larvae جدا شده از کلنی‌های زنبور عسل ایران

نوع مقاله : مقاله کامل

نویسندگان

1 بخش تشخیص بیماری‌های زنبور عسل و کرم ابریشم، موسسه تحقیقات واکسن و سرم‌سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 گروه زیست فناوری میکروبی، دانشکده علوم پایه دانشگاه علم و فرهنگ تهران، تهران، ایران

چکیده

بیماری لوک آمریکایی زنبورعسل یک بیماری باکتریایی کشنده لاروهای زنبورعسل در سراسر جهان است. این بیماری توسط باکتری گرم مثبت و تشکیل‌دهنده اسپور Paenibacillus larvae ایجاد می‌شود و زیان‌های اقتصادی مهمی را به همراه دارد. اما در مورد تنوع عامل ایجاد کننده تاکنون در ایران مطالعه‌ای صورت نگرفته است. در این تحقیق از 19 سویه ‌ Paenibacillus larvae که از لارو‌های بیمار، تلف شده و عسل، جداسازی شده بودند پس از تأیید بوسیله آزمایشات بیوشیمیایی، PCR ژن 16S rRNA و تعیین توالی، جهت استفاده در تکنیک ERIC-PCR برای بررسی تنوع ژنوتیپی استفاده شدند. نتایج امکان شناسایی ژنوتیپ‌های ERIC I و ERIC II را برای اولین بار در ایران فراهم کرد. 14 سویه‌ ژنوتیپ II (7/73 %) ‌و 5 سویه‌ ژنوتیپ I (3/26 %) را نشان دادند. 

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of genotypic diversity of Paenibacillus larvae strains isolated from Iranian honey bee colonies.

نویسندگان [English]

  • M moharrami 1
  • E Ahmadi Niri 2
1 Department of Honey Bee, Silk Worm a Wildlife, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
2 Department of Microbial Biotechnology, Faculty of Basic Sciences, University of Science and Culture, Tehran, Iran
چکیده [English]

American Foulbrood disease is a deadly bacterial disease of honey bee larvae in the world. The disease is caused by gram-positive and spores forming bacteria of Paenibacillus larvae that has significant economic losses. However, no study has been done on the diversity of this disease agent in Iran. In this study, 19 strains of Penibacillus larvae that were isolated from diseased and dead larvae and honey after confirmation by biochemical tests, PCR of 16S rRNA gene and sequencing, were used in ERIC-PCR technique to study genotypic diversity. The results enabled the identification of ERIC I and ERIC II genotypes for the first time in Iran.  14 strains of genotype II (73.7%) and 5 strains showed genotype I (26.3%).

کلیدواژه‌ها [English]

  • Paenibacillus larvae
  • American Foulbrood
  • (ERIC-PCR) technique
  • ERIC genotypes

مراجع

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سابقه مقاله

  • تاریخ دریافت: 29 دی 1400
  • تاریخ پذیرش: 23 بهمن 1400

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