اثر اسانس‌ مریم‌گلی و مرزه بر باکتری‌های استافیلوکوک آرئوس، استرپتوکوک آگالاکتیه و اشرشیاکلی

نوع مقاله : مقاله کامل

نویسندگان

گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس،گنبد کاووس، ایران

چکیده

برای درمان بیماری‌های باکتریایی با توجه به عوارض مصرف آنتی‌بیوتیک‌ها نیاز به مواد ضد‌باکتریایی جدید و طبیعی است و یکی از این مواد روغن‌های اسانسی گیاهان دارویی هستند لذا این مطالعه به‌منظور بررسی فعالیت ضد‌باکتریایی اسانس‌های مرزه و مریم‌گلی و مقایسه آن‌ها با آنتی‌بیوتیک‌های جنتامایسین و آموکسی‌سیلین علیه باکتری‌های استافیلوکوک آرئوس، استرپتوکوک آگالاکتیه و اشرشیاکلی انجام شد. شناسایی ترکیبات شیمایی اسانس با آنالیز به‌روش GC/MS انجام شد. حداقل غلظت بازدارنده رشد (MIC) و حداقل غلظت باکتری‌کشی (MBC) اسانس‌ها به روش رقیق‌سازی لوله‌ای و حساسیت باکتری به اسانس‌ها و آنتی‌بیوتیک‌ها با استفاده از دیسک دیفیوژن تعیین گردید و تاثیر اسانس‌ها بر منحنی رشد باکتری‌ها در ساعت‌های 0، 6، 10 و 24 مطالعه شد. مهم‌ترین ترکیبات مریم‌گلی کارواکرول(01/61%) و تیمول(41/20%) و مرزه اکالیپتول(45/32%) و کارواکرول( 24/28%) بود. از نظر MIC و MBC اسانس مریم‌گلی بیشترین تأثیر را علیه باکتری استافیلوکوک آرئوس (به‌ترتیب  009/0 % و 019/0%) و اسانس مرزه بیشترین تأثیر را علیه باکتری استرپتوکوک آگالاکتیه( به‌ترتیب 078/0% و 156/0%) داشت. در دیسک دیفیوژن نیز اثر ضد‌باکتریایی اسانس‌ها تفاوت معنی‌داری با جنتامایسین و آموکسی سیلین/کلاولانات نداشت و تاثیر مریم‌گلی قوی‌تر از مرزه بود. در منحنی رشد، غلظت MIC اسانس‌ها ساعت 24 باعث کاهش معنی‌دار تعداد باکتری‌ها شدند. به‌طور‌کلی اسانس مریم‌گلی با دارا بودن ترکیبات ضد‌باکتریایی قوی (کارواکرول و تیمول) بیشتر، اثر قوی‌تری از مرزه علیه باکتری‌ها داشت و اثر ضد‌باکتریایی هر دو اسانس تفاوت معنی‌داری با دو آنتی‌بیوتیک جنتامایسین و آموکسی سیلین/کلاولانات نداشت که نشان دهنده اثر قابل قبول اسانس‌هاست. 

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Essential Oils of Salvia officinalis and Satureja hortensis on Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli

نویسندگان [English]

  • R. Rahchamani
  • S. Zarooni
  • F. Ghanbari
Department of Animal Science, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran
چکیده [English]

There is need to investigate new and natural antibacterial agents because of various side effects of antibiotics. Essential oil of medicinal plants is one of these agents. The aim of this study was to determine the antibacterial activity of Salvia officinalis (sage) and Satureja hortensis (savory) essential oils in comparison with Gentamicin and Amoxicillin / Clavulanate antibiotics on Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli. Chemical compositions of essential oils were determined by GC/MS. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) of oils and the inhibition zones of oils and antibiotics were determined using serial broth dilution and disk diffusion method, respectively. The effect of MIC concentrations of oils on growth curve of bacteria were obtained at 0, 6, 10 and 24 hour. Major compositions of sage and savory oils were carvacrol (61.01%), thymol (20.41%) and eucalyptol (32.45%), thymol (28.24%), respectively. According to MIC and MBC results, the most antibacterial activity of sage was against Staphylococcus aureus (0.009% and 0.019%, respectively) and those of savory were against Streptococcus agalactiae (0.078%, 0.156%, respectively). The inhibition zone of oils had no significant difference with antibiotics and antibacterial effect of sage was more than savory. MIC concentrations of oils decreased bacterial count at 24 h. Generally, the essential oil of sage had a stronger antibacterial effect than savory because of more strong antibacterial components (carvacrol and thymol) and Antibacterial effects of two oils had no significant difference with gentamycin and amoxicillin/ clavulanate that show satisfactory antibacterial effect of oils.

کلیدواژه‌ها [English]

  • Antibacterial
  • Antibiotic resistance
  • Medicinal plant
  • Bacterial disease
  • Mastitis

مراجع

1.Adrar, N., N. Oukil and F. Bedju. 2016. Antioxidant and antibacterial activities of Thymus numidicus and Salvia officinalis essential oils alone or in combination. Industrial Crops and Products 88: 112-119.
2.Alekish, M.O., Z.B. Ismail, M.S. Awawdeh and S. Shatnawi. 2017. Effects of intramammary infusion of sage (Salvia officinalis) essential oil on milk somatic cell count, milk composition parameters and selected hematology and serum biochemical parameters in Awassi sheep with subclinical mastitis. Veterinary World 10(8): 895-900.
3.Ananda Baskaran, S., G.W. Kazmer, L. Hinckley, S.M. Andrew and K. Venkitanarayanan. 2009. Antibacterial effect of plant-derived antimicrobials on major bacterial mastitis pathogen’s in vitro. Journal of Dairy Science 92(4): 1423-1429.
4.Aumeeruddy-elalfi, Z., A. Gurib-fakim and F. Mahomoodally. 2015. Antimicrobial, antibiotic potentiating activity and phytochemical profile of essential oils from exotic and endemic medicinal plants of Mauritius. Industrial Crops & Products 71: 197-204.
5.Bouyahya, A., A. Et-Touys, J. Abrini, A. Talbaoui, H. Fellah, Y. Bakri and N. Dakka. 2017. Lavandula stoechas essential oil from Morocco as novel source of antileishmanial, antibacterial and antioxidant activities. Biocatalysis and Agricultural Biotechnology 12: 179-184.
6.Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology 94: 223-253.
7.CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25, Wayne.
8.Elshafie hazem, S., S. Sakr, S.M. Mang and S. Belviso. 2016. Antimicrobial activity and chemical composition of three essential oils extracted from Mediterranean aromatic plants. Journal Of Medicinal Food 19(11): 1-8.
9.Essid, R., F. Zahra, K. Msaada, I. Sghair, M. Hammami, A. Bouratbine, K. Aoun and F. Limam. 2015. Antileishmanial and cytotoxic potential of essential oils from medicinal plants in Northern Tunisia. Industrial Crops & Products 77: 795-802.
10.Fu, Y., Y. Zu, L. Chen, , X. Shi, , Z. Wang, S. Sun and T. Efferth. 2007. Antimicrobial Activity of Clove and Rosemary Essential Oils Alone and in Combination. Phytotherapy Research 994 March: 989-994.
11.Golparvar, A.R., M.M. Gheisari, A. Hadipanah and M. Khorrami. 2018. Antibacterial, Antifungal Properties and Chemical Composition of Essential Oils of Satureja hortensis L. and Satureja khuzestanica Jamzad. Journal of Herbal Drugs, 8(4): 243-249.
12.Hajlaoui, H., H. Mighri, M. Aouni, N. Gharsallah and A. Kadri. 2016. Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase properties of Tunisian Origanum majorana L. essential oil. Microbial Pathogenesis 95: 86-94.
13.Jafari, F., F. Farmani, K. Zomorodian, M. Moein, P. Faridi and M.M. Zarshenas. 2018. A Study on Essential Oil Chemical Compositions, Antioxidant, and Antimicrobial Activities of Native and Endemic Satureja Species Growing in Iran. Pharmaceutical Chemistry Journal 52(1): 63-68.
14.Misaghi, A. and A.A. Basti. 2007. Effects of Zataria multiflora Boiss. essential oil and nisin on Bacillus cereus ATCC 11778. Food Control 18(9):1043-1049.
15.Montironi, I.D., L.N. Cariddi and E.B. Reinoso. 2016. Evaluation of the antimicrobial efficacy of Minthostachys verticillata essential oil and limonene against Streptococcus uberis strains isolated from bovine mastitis. Revista Argentina de Microbiología 48(3): 210-216.
16.Moosavi-Nasab, M., M.J. Saharkhiz, E. Ziaee, F. Moayedi, R. Koshani and R. Azizi. 2016. Chemical compositions and antibacterial activities of five selected aromatic plants essential oils against food-borne pathogens and spoilage bacteria. Essential Oil Research 83(3): 607-613.
17.Mozafari, S., A. Seidavi, S. Gharahveysi and I. Kadim. 2018. Savory (Satureja hortensis L.) powder and extract effects on broiler chicken ileal Escherichia coli and lactobacillus bacteria. Journal of Applied Animal Research 46(1): 639-642.
18.Park, Y.K., L.K. Fox, D.D. Hancock, W. McMahan and Y.H. Park. 2012. Prevalence and antibiotic resistance of mastitis pathogens isolated from dairy herds transitioning to organic management. Journal of Veterinary Science 13(1): 103-105.
19.Raffaella, C., L. Casettari, L. Fagioli, M. Cespi, G. Bonacucina and W. Baffone. 2017. Activity of essential oil-based microemulsions against Staphylococcus aureus biofilms developed on stainless steel surface in different culture media and growth conditions. International Journal of Food Microbiology 241: 132-140.
20.Russo, A., C. Formisano, D. Rigano, F. Senatore, S. Delfine, V. Cardile, S. Rosselli and M. Bruno. 2013. Chemical composition and anticancer activity of essential oils of Mediterranean sage ( Salvia officinalis L.) grown in different environmental conditions. Food And Chemical Toxicology 55: 42-47.
21.Sharifi, A., A. Mohammadzadeh, T. Zahraei Salehi and P. Mahmoodi. 2018. Anti-bacterial, anti-biofilm and anti-quorum sensing effects of Thymus daenensis and Satureja hortensis essential oils against Staphylococcus aureus isolates. Journal of Applied Microbiology 124(2): 379-388.
22.Solórzano-Santos, F. and M.G. Miranda-Novales. 2012. Essential oils from aromatic herbs as antimicrobial agents. Current Opinion in Biotechnology 23(2): 136-141.
23.Stefanovi, O.D., D.D. Stanojevi and L.R. Omi. 2012. Synergistic Antibacterial Activity Of Salvia Officinalis And Cichorium Intybus Extracts And Antibiotics. Natural Drugs 69(3): 457-463.
24.Suntres, Z.E., J. Coccimiglio and M. Alipour. 2015. The Bioactivity and Toxicological Actions of Carvacrol. Critical Reviews in Food Science and Nutrition 55(3): 304-318.
25.Tepe, B. and M. Cilkiz. 2016. A pharmacological and phytochemical overview on Satureja. Pharmaceutical Biology 54(3): 375-412.
26.Zhu, H., M. Du, L. Fox and M.J. Zhu. 2016. Bactericidal effects of Cinnamon cassia oil against bovine mastitis bacterial pathogens. Food Control 66: 291-299.

فایل ها

سابقه مقاله

  • تاریخ دریافت: 07 بهمن 1398
  • تاریخ پذیرش: 19 فروردین 1399

هم رسانی

ارجاع به این مقاله

آمار