اثر اسانس‌ مریم‌گلی و مرزه بر باکتری‌های استافیلوکوک آرئوس، استرپتوکوک آگالاکتیه و اشرشیاکلی

نوع مقاله : مقاله کامل

نویسندگان

گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس،گنبد کاووس، ایران

چکیده

برای درمان بیماری‌های باکتریایی با توجه به عوارض مصرف آنتی‌بیوتیک‌ها نیاز به مواد ضد‌باکتریایی جدید و طبیعی است و یکی از این مواد روغن‌های اسانسی گیاهان دارویی هستند لذا این مطالعه به‌منظور بررسی فعالیت ضد‌باکتریایی اسانس‌های مرزه و مریم‌گلی و مقایسه آن‌ها با آنتی‌بیوتیک‌های جنتامایسین و آموکسی‌سیلین علیه باکتری‌های استافیلوکوک آرئوس، استرپتوکوک آگالاکتیه و اشرشیاکلی انجام شد. شناسایی ترکیبات شیمایی اسانس با آنالیز به‌روش GC/MS انجام شد. حداقل غلظت بازدارنده رشد (MIC) و حداقل غلظت باکتری‌کشی (MBC) اسانس‌ها به روش رقیق‌سازی لوله‌ای و حساسیت باکتری به اسانس‌ها و آنتی‌بیوتیک‌ها با استفاده از دیسک دیفیوژن تعیین گردید و تاثیر اسانس‌ها بر منحنی رشد باکتری‌ها در ساعت‌های 0، 6، 10 و 24 مطالعه شد. مهم‌ترین ترکیبات مریم‌گلی کارواکرول(01/61%) و تیمول(41/20%) و مرزه اکالیپتول(45/32%) و کارواکرول( 24/28%) بود. از نظر MIC و MBC اسانس مریم‌گلی بیشترین تأثیر را علیه باکتری استافیلوکوک آرئوس (به‌ترتیب  009/0 % و 019/0%) و اسانس مرزه بیشترین تأثیر را علیه باکتری استرپتوکوک آگالاکتیه( به‌ترتیب 078/0% و 156/0%) داشت. در دیسک دیفیوژن نیز اثر ضد‌باکتریایی اسانس‌ها تفاوت معنی‌داری با جنتامایسین و آموکسی سیلین/کلاولانات نداشت و تاثیر مریم‌گلی قوی‌تر از مرزه بود. در منحنی رشد، غلظت MIC اسانس‌ها ساعت 24 باعث کاهش معنی‌دار تعداد باکتری‌ها شدند. به‌طور‌کلی اسانس مریم‌گلی با دارا بودن ترکیبات ضد‌باکتریایی قوی (کارواکرول و تیمول) بیشتر، اثر قوی‌تری از مرزه علیه باکتری‌ها داشت و اثر ضد‌باکتریایی هر دو اسانس تفاوت معنی‌داری با دو آنتی‌بیوتیک جنتامایسین و آموکسی سیلین/کلاولانات نداشت که نشان دهنده اثر قابل قبول اسانس‌هاست. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Essential Oils of Salvia officinalis and Satureja hortensis on Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli

نویسندگان [English]

  • R. Rahchamani
  • S. Zarooni
  • F. Ghanbari
Department of Animal Science, College of Agriculture and Natural Resources, University of Gonbad Kavous, Gonbad Kavous, Iran
چکیده [English]

There is need to investigate new and natural antibacterial agents because of various side effects of antibiotics. Essential oil of medicinal plants is one of these agents. The aim of this study was to determine the antibacterial activity of Salvia officinalis (sage) and Satureja hortensis (savory) essential oils in comparison with Gentamicin and Amoxicillin / Clavulanate antibiotics on Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli. Chemical compositions of essential oils were determined by GC/MS. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) of oils and the inhibition zones of oils and antibiotics were determined using serial broth dilution and disk diffusion method, respectively. The effect of MIC concentrations of oils on growth curve of bacteria were obtained at 0, 6, 10 and 24 hour. Major compositions of sage and savory oils were carvacrol (61.01%), thymol (20.41%) and eucalyptol (32.45%), thymol (28.24%), respectively. According to MIC and MBC results, the most antibacterial activity of sage was against Staphylococcus aureus (0.009% and 0.019%, respectively) and those of savory were against Streptococcus agalactiae (0.078%, 0.156%, respectively). The inhibition zone of oils had no significant difference with antibiotics and antibacterial effect of sage was more than savory. MIC concentrations of oils decreased bacterial count at 24 h. Generally, the essential oil of sage had a stronger antibacterial effect than savory because of more strong antibacterial components (carvacrol and thymol) and Antibacterial effects of two oils had no significant difference with gentamycin and amoxicillin/ clavulanate that show satisfactory antibacterial effect of oils.

کلیدواژه‌ها [English]

  • Antibacterial
  • Antibiotic resistance
  • Medicinal plant
  • Bacterial disease
  • Mastitis
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