سنجش خواص آنتی اکسیدانی اسیدین (Phallusia nigra) دریای عمان

نوع مقاله : مقاله کامل

نویسندگان

1 دانشیار فرآوری محصولات شیلاتی، دانشگاه دریانوردی و علوم دریایی چابهار

2 دانشجوی دکتری فرآوری محصولات شیلاتی، دانشگاه دریانوردی و علوم دریایی چابهار

چکیده

امروزه بررسی ترکیبات آنتی‌اکسیدان طبیعی مستخرج از موجودات دریایی مورد توجه می‌باشد. لذا در این مطالعه خواص آنتی اکسیدانی اسیدین Phallusia nigra سواحل چابهار بررسی شد. خواص آنتی اکسیدانی با استفاده از روش مهار رادیکال آزاد Diphenyl Picrylhydrazyl (DPPH)i، قدرت کاهشی و فعالیت کلاته‌کنندگی با استفاده از حلال‌های متانول، اتانول و کلروفرم بررسی شد. از آنالیز واریانس یک طرفه و آزمون تعقیبی دانکن جهت آنالیز آماری استفاده شد. بیشترین درصد مهار رادیکال DPPH مربوط به غلظت mg/ml 1 عصاره متانولی با مهار 5/1± 09/93 درصد بود. در روش سنجش قدرت کاهشی، غلظت mg/ml 1 عصاره متانولی بیش از سایر عصاره‌ها بود (01/0±35/0). فعالیت کلاته‌کنندگی عصاره کلروفرمی در همه غلظت‌ها بالاترین درصد را داشت. در هر سه روش رابطه مستقیمی بین غلظت، توانایی مهار و جذب عصاره‌ها برقرار بود. نتایج نشان داد خواص آنتی اکسیدانی Phallusia nigra بسیار قابل توجه و می‌تواند مبنایی برای انجام مطالعات بعدی به منظور دستیابی به مواد دارویی جدید برای درمان بسیاری بیماری‌ها از جمله سرطان باشد. همچنین پس از انجام مطالعات درون تنی، پیش کلینیکی و کلینیکی می‌توان از عصاره آن در صنایع آرایشی و بهداشتی و دامپزشکی استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation the antioxidative properties of the sea squirt Ascidian (Phallusia nigra) from Oman Sea

نویسندگان [English]

  • A. Taheri 1
  • Z. Bavi 2
1 Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
2 Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
چکیده [English]

Antioxidants are free radical scavengers, which protect the body against oxidative damages. Marine organism have also attracted attention in research on natural antioxidants. Therefore antioxidative properties of ascidian (Phallusia nigra) extracts from Oman Sea were investigated. DPPH free radical scavenging, reduction power and metal chelating activity were employed to identify the antioxidant properties by using methanol, ethanol and chloroform solvents. Experimental results of this study were presented as mean ± standard deviation of three replications and for analyzing data were analyzed by one-way ANOVA and Duncan multiple range test. The concentration of (1 mg/ml) methanol gained the highest percentage of DPPH radical scavenging (i.e. 93.09 ± 1.5 %). In reduction power assay, concentration of (1 mg/ml) methanol (0.35 ± 0.01) was higher in comparison with other extracts. In all the concentrations, Metal chelating activity of chloroform extract was reported to have the highest percentage. In all three assays, there was a direct relationship between concentration, control and absorption power of extract. In this study, the antioxidant properties of Phallusia nigra showed to be significant and could be used for further studies in order to find new pharmaceutical compounds for treating many diseases like cancer. Moreover After preclinical and clinical studies, the extract can be used in cosmetic and veterinary medicine industries.

کلیدواژه‌ها [English]

  • free radical
  • antioxidant
  • Phallusia nigra
  • DPPH
  • extract

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سابقه مقاله

  • تاریخ دریافت: 07 آذر 1396
  • تاریخ بازنگری: 04 اردیبهشت 1398
  • تاریخ پذیرش: 17 اردیبهشت 1398

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