سنجش خواص آنتی اکسیدانی اسیدین (Phallusia nigra) دریای عمان

نوع مقاله : مقاله کامل

نویسندگان

1 دانشیار فرآوری محصولات شیلاتی، دانشگاه دریانوردی و علوم دریایی چابهار

2 دانشجوی دکتری فرآوری محصولات شیلاتی، دانشگاه دریانوردی و علوم دریایی چابهار

چکیده

امروزه بررسی ترکیبات آنتی‌اکسیدان طبیعی مستخرج از موجودات دریایی مورد توجه می‌باشد. لذا در این مطالعه خواص آنتی اکسیدانی اسیدین Phallusia nigra سواحل چابهار بررسی شد. خواص آنتی اکسیدانی با استفاده از روش مهار رادیکال آزاد Diphenyl Picrylhydrazyl (DPPH)i، قدرت کاهشی و فعالیت کلاته‌کنندگی با استفاده از حلال‌های متانول، اتانول و کلروفرم بررسی شد. از آنالیز واریانس یک طرفه و آزمون تعقیبی دانکن جهت آنالیز آماری استفاده شد. بیشترین درصد مهار رادیکال DPPH مربوط به غلظت mg/ml 1 عصاره متانولی با مهار 5/1± 09/93 درصد بود. در روش سنجش قدرت کاهشی، غلظت mg/ml 1 عصاره متانولی بیش از سایر عصاره‌ها بود (01/0±35/0). فعالیت کلاته‌کنندگی عصاره کلروفرمی در همه غلظت‌ها بالاترین درصد را داشت. در هر سه روش رابطه مستقیمی بین غلظت، توانایی مهار و جذب عصاره‌ها برقرار بود. نتایج نشان داد خواص آنتی اکسیدانی Phallusia nigra بسیار قابل توجه و می‌تواند مبنایی برای انجام مطالعات بعدی به منظور دستیابی به مواد دارویی جدید برای درمان بسیاری بیماری‌ها از جمله سرطان باشد. همچنین پس از انجام مطالعات درون تنی، پیش کلینیکی و کلینیکی می‌توان از عصاره آن در صنایع آرایشی و بهداشتی و دامپزشکی استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation the antioxidative properties of the sea squirt Ascidian (Phallusia nigra) from Oman Sea

نویسندگان [English]

  • A. Taheri 1
  • Z. Bavi 2
1 Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
2 Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
چکیده [English]

Antioxidants are free radical scavengers, which protect the body against oxidative damages. Marine organism have also attracted attention in research on natural antioxidants. Therefore antioxidative properties of ascidian (Phallusia nigra) extracts from Oman Sea were investigated. DPPH free radical scavenging, reduction power and metal chelating activity were employed to identify the antioxidant properties by using methanol, ethanol and chloroform solvents. Experimental results of this study were presented as mean ± standard deviation of three replications and for analyzing data were analyzed by one-way ANOVA and Duncan multiple range test. The concentration of (1 mg/ml) methanol gained the highest percentage of DPPH radical scavenging (i.e. 93.09 ± 1.5 %). In reduction power assay, concentration of (1 mg/ml) methanol (0.35 ± 0.01) was higher in comparison with other extracts. In all the concentrations, Metal chelating activity of chloroform extract was reported to have the highest percentage. In all three assays, there was a direct relationship between concentration, control and absorption power of extract. In this study, the antioxidant properties of Phallusia nigra showed to be significant and could be used for further studies in order to find new pharmaceutical compounds for treating many diseases like cancer. Moreover After preclinical and clinical studies, the extract can be used in cosmetic and veterinary medicine industries.

کلیدواژه‌ها [English]

  • free radical
  • antioxidant
  • Phallusia nigra
  • DPPH
  • extract
. Afkhami, M., M. Ehsanpour, F. Forouzan, K. Darvish Bastami, A.H. Bahri and A. Daryaei. 2012. Distribution of ascidians Phallusia nigra (Tunicata: Ascidiacea) on the north coast of the Persian Gulf, Iran. Marine Biodiversity Records 5:91-95.
2. Chang, L.W., W.J. Yen, S.C. Huang and P.D. Duh. 2002. Antioxidant activity of sesame coat. Food Chemistry 78: 347–354.
3. Cespecles, C.L., M. EI-Hafidi, N. Pavon and J. Alarcon. 2008. Antixoidant and cardioprotective activities of phenolic extracts from fruits of Chilean blackberry Aristotelia chilensis (Elaeocarpaceae) Maqui. Food Chemistry 107: 820-829.
4. Chia Ling, J. and K.O. Wen-ching. 2002. 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging by protein hydrolyzates from tuna cooking juice. Fisheries Science 68: 430–435.
5. Cho, M., H.S. Lee, I.J. Kang and S.G. You. 2011. Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chemistry 127: 999-1006.
6. Cooper E. L. and D. Yao. 2012. Diving for drugs: tunicate anticancer compounds. Drug Discovery Today 7(11-12): 636- 648.
7. Davis A.R. and J.B. Bremner. 1999. Potential antifouling natural products from ascidian: A review. Bioactive compounds from marine organisms. Oxford & IBH Publishing Co. Pvt. Ltd, New Delhi: 259-310.
8. Findlay C. and V. J. Smith. 1995. Antimicrobial factors in solitary ascidians. Fish & Shellfish Immunology 5: 645-658.
9. Guedes, É.A., T.G. da Silva, J.S. Aguiar, L.D. de Barros, L.M. Pinotti and A.E. Sant’Ana. 2013. Cytotoxic activity of marine algae against cancerous cells. Brazilian Journal of Pharmacognosy 23: 668-673.
10. Gharanjik, B.M., M. Wynne, X. Bangmei, S. Khajeh, H. Keyanmehr and M.R. Hosseini. 2011. The biomass of the medicinal red algae (Rhodophyta) in the intertidal zone of the Chabahar coasts. Iranian Journal of Fisheries Sciences 20: 103-114. [in Farsi].
11. Ganesan, K., S.K. Kumar and R.P. Subba. 2011. Comparative assessment of antioxidant activity in three edible species of green seaweed, Enteromorpha from Okha, Northwest coast of India. Innovative Food Sci. Emerging Technol 12: 73-78.
12. Halliwell B. 2007. Dietary polyphenols: Good, bad, or indifferent for your health?. Cardiovascular Research 73: 341–347.
13. Jaffarali, H.A., M. Tamilselvi and V. Sivakumar. 2008. Antibacterial activity of the marine ascidians Phallusia nigra and Herdmania pallida from the Tuticorin coast, India and Herdmania pallida from the Tuticorin coast. Journal of Biological Research-Thessaloniki 10: 171 – 179.
14. Kim, S.K. and R. Pallela. 2012. Medicinal Foods from Marine Anim als: Current Status and Prospects. Advances in Food and Nutrition Research 65: 1-9.
15. Lim, S.N., P.C. Cheung, V.E. Ooi and P.O. Ang. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed Sargassum siliquastrum. Journal of Agricultural and Food Chemistry (50): 3862–6.
16. Magalhaes, L.M., M.A. Segundo, S. Reis and JL. Lima. 2008. Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta 613: 1–19.
17. Meir, S., J. Kanner, B. Akiri and P. Hadas. 1995. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. Journal of Agricultural and Food Chemistry 43: 1813–1819.
18. Nazarian, M., S.J. Hosseini, I. Nabipour and G.H. Mohebbi. 2015. Marine bioactive peptides with anti-cancer potential. ISMJ 18: 607- 29. [in Farsi]
19. Peymani, J., A. Gharaei, M. Ghaffari and A. Taheri. 2013. Antibacterial activity of the brown algae (Sargassum glaucescens) ethanolic and aqueous extracts from Chabahar coasts, Oman Sea, Iran. Iranian Scientific Fisheries Journal 4: 13-21. [in Farsi]
20. Rocha, R.M.D., T.M.D.C. Lotufo and S.D.A. Rodrigues. 1999. The biology of Phallusia nigra Savigny, 1816 (Tunicata: Ascidiacea) in southern Brazil: Spatial distribution and reproductive cycle. Bulletin of Marine Science 64: 77-87.
21. Priya, S.D., H.K.S. Christy, S. Sankaravadivu and C.S. Packiam. 2015. Antioxidant activity of the simple ascidian Phallusia nigra of Thoothukudi Coast. International Journal of Pharmaceutical Chemistry 12: 410-412.
22. Suresh Kumar, K., K. Ganesan and P. Subba Rao. 2007. Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty – An edible seaweed. Food Chemistry 107: 289–295.
23. Suganthy, N., S. Arif Nisha and S. Karutha. 2013. Evaluation of Gelidiella acerosa, the red algae inhabiting South Indian coastal area for antioxidant and metal chelating potential. Biomedicine & Preventive Nutrition 3: 399-406.
24. Sugumaran, M. and W.E. Robinson. 2012. Structure, biosynthesis and possible function of tunichromes and related compounds. Comparative Biochemistry and Physiology – Part B: Biochemistry & Molecular Biology 163: 1–25.
25. Zorofchian Moghadamtousi, S., H. Karimian, R. Khanabdali, M. Razavi, M. Firoozinia and K. Zandi. 2014. Anticancer and Antitumor Potential of Fucoidan and Fucoxanthin, Two Main Metabolites Isolated from Brown Algae. The Scientific World Journal 3:1-10.