اثر مکمل روی و جایگزینی بتائین جیره با متیونین بر عملکرد و فراسنجه‌های خونی مرغان تخمگذار در شرایط استرس گرمایی

نوع مقاله : مقاله کامل

نویسندگان

1 استادیار گروه علوم دامی –دانشکده علوم دامی و صنایع غذایی- دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، اهواز، ایران

2 دانشیار گروه علوم دامی –دانشکده علوم دامی و صنایع غذایی- دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، اهواز، ایران

3 دانشیار گروه علوم دامی –دانشکده علوم دامی و صنایع غذایی- دانشگاه علوم کشاورزی و منابع طبیعی خوزستان

چکیده

به منظور بررسی تاثیر سطوح مختلف بتائین جایگزین‌شده با متیونین و مکمل روی بر عملکرد و فراسنجه‌های خونی در مرغان تخم‌گذار لگهورن سویه تجاری های–‌لاین (W-36)، آزمایشی با استفاده از 288 قطعه مرغ تخم‌گذار در قالب طرح کاملاً تصادفی به صورت فاکتوریل با 12 تیمار آزمایشی، 3 تکرار و 8 قطعه مرغ در هر تکرار از سن 50 الی 62 هفتگی اجرا شد. تیمارهای آزمایشی شامل سه سطح مختلف جایگزینی بتائین با متیونین (صفر و 13 و 26 درصد) و دو سطح مختلف مکمل روی (50 و 100 میلی‌گرم در کیلوگرم سولفات روی) در دو شرایط دمایی متفاوت (استرس گرمایی و بدون استرس) با سه تکرار در هر تیمار بودند. نتایج آزمایش نشان داد که صفات عملکردی و صفات کیفی تخم‌مرغ در تیمار تحت استرس گرمایی نسبت به تیمار بدون استرس به طور معنی‌داری کاهش یافتند. جایگزینی بتائین با متیونین سبب افزایش تولید تخم‌مرغ گردیده است (05/0>P). افزودن مکمل روی به جیره به طور معنی‌داری سبب افزایش پارامترهای عملکردی و صفات کیفی تخم‌مرغ گردید (05/0>P). بعلاوه، اثر بتایین بر کلسترول و تری‌گلیسرید خون معنی‌دار بود ولی بر میزان گلوکز خون اثری نداشت. همچنین، افزودن عنصر روی سبب کاهش گلوکز، کلسترول و تری‌گلیسرید گردید (05/0>P). نتایج این مطالعه نشان داد که جایگزین‌نمودن بتایین به جای متیونین به میزان 13 درصد و همچنین افزودن مکمل روی به میزان 100 میلی‌گرم در کیلوگرم به جیره مرغ تخم‌گذار تحت تنش گرمایی سبب بهبود عملکرد می‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Zinc supplementation and Betaine substitution to methionine on performance and blood parameters of laying hens under heat stress

نویسندگان [English]

  • mahmood nazari 1
  • Somayeh Sallary 2
  • Mohammad Reza Ghorbani 3
1 Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran.
2 Associate Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran.
3 Associate Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran.
چکیده [English]

To investigate the effects of dietary zinc and betaine (Bet) substitution to methionine on performance, egg quality and blood parameters in laying hens under heat stress, an experiment with 288 Hy-line W-36 leghorn (at 50 to 62 weeks old) in complete randomized design (CRD) with a 3×2×2 factorial arrangement of treatments with twelve treatments, 3 replicates and 8 hens in each replicate was performed. There were 6 dietary treatments: three doses of Bet (0, 13 and 26%) substitute to methionine combined with three levels of zinc (50 and 100 mg/kg) supplemented to the basal diets that were combined with two environmental conditions (thermoneutral (23°C constant) and heat stress ( 23.9 to 35°C cycling) conditions). The results indicated that performance parameters of laying hens and egg quality were all significantly reduced by heat stress (HS) (P<0.05). Substitution of methionine with Bet led to significantly increase of egg production (P<0.05); whereas it had no effect on egg weight and egg quality. Supplementation of the basal diets with zinc significantly increased laying performance of hens and egg quality (P<0.05). Moreover, the results of this experiment indicated that, there were significant effect of Betaine on blood cholesterol and triglyceride, (p<0.05), but no significant effect on glucose in broiler chickens. Moreover, results demonstrated that Zinc supplementation decreased cholesterol, triglyceride and glucose. The present study showed that dietary supplemental Bet and zinc at the level of 13% and 100 mg/kg resulted in an improving egg quality and performance of laying hens under heat stress.

کلیدواژه‌ها [English]

  • Blood parameters
  • Heat stress
  • Zinc supplementation
  • betaine
1- Balnave, D., and Zhang, D. 1993. Response of laying hens on saline drinking water to dietary supplementation with various Zn com pounds. Poultry Science 72: 603–609.
2- Domingues, C. and Sgavioli S. 2012. Lysine and methionine + cystine for laying hens during the post-molting phase. The journal Revista Brasileira de Ciência Avícola. vol.14, n.3, pp. 187-192. 
3- Enting, H., Eissen, J. and Delos Mozos, J. 2005. Betaine improves broiler chicken performance and carcass quality under heat stress conditions. 16th European Symposium on Poultry Nutrition 297-300.
4- Feng, J., Liu, X., Wang, Y. Z. and Xu, Z. R. 2006. Effects of betaine on performance, carcass characteristics and hepatic betaine-homocysteine methyl transferase activity in finishing barrows. Asian-Aust. Journal of Animal Science 19: 402-405.
5- Garriga, C., Richard, R., Hunter, C., Amat, J., Planas, M., Malcolm, A., Moreto, M. and Moreto, M. 2006. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 290: R195–R201.
6- Guo, Y.M., Yang, R., Yuan, J., Ward, T.L. and Fakler, T.M. 2002. Effect of Availa Zn and ZnSO4 on laying hen perform‌ance and egg quality. Poultry Science 81 (Suppl.), 40.
7- Johannes, W. G., Swinkelsiz, M., Ervin, T., Kornegay, W. and Verstegen, A. 1994. Biology of Zinc and Biological Value of Dietary Organic Zinc Complexes and Chelates. Nutrition Research Reviews 7: 129-149.
8- Kucuk, O., Kahraman, A. Kurt, I. Yildiz, N. and Onmaz A. C. 2008. A combination of zinc and pyridoxine supplementation to the diet of laying hens improves performance and egg quality. Biol. Trace Elem. Res. 126:165–175.
9- Kirunda, D. F., Scheideler, S. E. and McKee, S. R. 2001. The efficacy of vitamin E (DL-alpha-tocopheryl acetate) supplementation in hen diets to alleviate egg quality deterioration associated with high temperature exposure. Poultry Science 80: 1378–1383.
10- Lu, J. J. and Zou, X. T. 2006. Effects of adding betaine on laying performance and contents of serum yolk precursors VLDL and VTG in laying hen. J. Zhejiang Univ. (Agric. Life Sci.) 32:287-291.
11- Mashaly, M. M., Hendricks, G. L. Kalama, M. A. Gehad, A. E. Abbas, A. O. and Patterson, P. H. 2004. Effect of Heat Stress on Production Parameters and Immune Responses of Commercial Laying Hens. Poultry Science 83:889–894. 
12- Nys, Y., Gautron, J. McKee, M. D., Garcia-Ruiz, J. M. and Hincke, M. 2001. Biochemical and functional characterization of eggshell matrix proteins. World’s Poultry Science Journal 57:401–403.
13- Park, J. H., Kang, C. W. and Ryu, K. S. 2006. Effects of feeding betaine on performance and blood hormone in laying hens. Korean Journal Poultry Science 33: 323-328.
14- Rashidi, A. A., Gofrani Ivari, Y., Khatibjoo, A. and Vakili, R. 2010. Effect of Dietary Fat, Vitamin E and Zinc on Immune Response and Blood Parameters of Broiler Reared Under Heat Stress. Research Journal of Poultry Science 3(2): 32-38.
15- Sahin, R., and Kucuk, O. 2001. A simple way to reduce heat stress in laying hens as judged by egg laying, body weight gain and biochemical parameters. Acta Vetenarium Hungarcia 49: 421–430.
16- Sahin, K. and Kucuk, O. 2003. Zinc supplementation alleviates heat stress in laying Japanese quail. Journal Nutrition 33: 2808–2811.
17- Star, L., Decuypere, E., Parmentier, H. K. and Kemp, B. 2008. Effect of Single or Combined Climatic and Hygienic Stress in Four Layer. Poultry Science 87: 1031–1038.
18- Sun, H., Yang, R., Yang, Z. B., Wang, Y., Jiang, S. Z. and Zhang, G. G. 2008. Effects of betaine supplementation to methionine deficient diet on growth performance and carcassa characteristics of broilers. American Journal of Animal and Veterinary Sciences 3 (3): 78-84.
19- Swiatkiewicz, S. Koreleski, J. 2008. The effect of zinc and manganese source in the diet for laying hens on eggshell and bones quality. Veterinarni Medicina 53 (10): 555–563.
20- Tucker, L. A. and Remus, J. 2001. The effect of betaine on performance, water balance and gut integrity of coccidiosis-infected poultry and its potentioal benefit in AGP-free diets. British Poultry Science 42(1): 108-109.
21- Ueland, P. M., Holm, P. and Hustad, S. 2005. Betaine: a key modulator of one-carbon metabolism and homocysteine status. Clin Chem Lab Med 43(10):1069–1075.
22- Zamani, A., Rahmani, H. R., Pourreza, H. R. 2005. Eggshell quality is improved by excessive dietary zinc and manganese. In: Proceedings 15th European Symposium on Poultry Nutrition 520–522.
23- Zhan, X. A. 2000. Studies on growth-promoting mechanism of betaine in broiler chickens. Acta Agric. Zhejiangensis 12: 209-212.
24- Zou, X. T., Ma Y. L. and Z. R. Xu. 1998. Effects of betaine and thyroprotein on laying performance and approach to mechanism of the effects in hens. Acta Agric Zhejiang 10:144-149.