اثر کروسین بر هورمون‌های محور هیپوفیز- تخمدان در مدل موش‌های صحرایی دیابتی نوع یک

نوع مقاله: مقاله کامل

نویسندگان

استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه پیام نور، تهران، ایران

چکیده

دیابت ملیتوس یک بیماری متابولیک است که با اختلال در عملکرد غدد اندوکرین سبب بروز اختلالات باروری می‌شود. با توجه به نقش کروسین در تعدیل سیستم دفاع آنتی‌اکسیدانی، هدف از این مطالعه تعیین اثر کروسین بر هورمون‌های محور هیپوفیز- تخمدان در مدل موش‌های صحرایی دیابتی نوع یک انجام شد. در این مطالعه تجربی، 28 سر موش صحرایی ماده نژاد ویستار به 4 گروه‌ مساوی تقسیم شدند. گروه‌های شاهد، شاهد دیابتی و دو گروه‌‌ دیابتی تحت تیمار با کروسین (غلظت‌های 50 و 100 میلی‌گرم بر کیلوگرم). دیابت در گروه‌های شاهد دیابتی و دیابتی تحت تیمار، با یک‌بار تزریق داخل صفاقی آلوکسان القاء شد. چرخه استروس موش‌های صحرایی توسط هورمون‌های جنسی یکسان شد. کروسین به مدت 25 روز به‌صورت داخل صفاقی به گروه‌های دیابتی تحت تیمار تزریق شد. به حیوانات گروه‌های شاهد و شاهد دیابتی محلول سالین تزریق شد. در پایان دوره تیمار، غلطت سرمی LH، FSH، استروژن و پروژسترون توسط روش الایزا اندازه‌گیری شد. تحلیل آماری توسط آزمون‌های One-way ANOVA و تعقیبی Tukey انجام شد. در مقایسه با گروه شاهد دیابتی، تجویز وابسته به دوز کروسین با غلظت‌های 50 و 100 میلی‌گرم بر کیلوگرم غلظت سرمی هورمون‌های LH، FSH، استروژن و پروژسترون را به‌طور معنی‌داری افزایش داد (05/0>p). نتایج این آزمایش نشان داد کروسین با افزایش سطح سرمی هورمون‌های LH، FSH استروژن و پروژسترون احتمالا موجب بهبود اختلالات هورمونی در بیماران دیابتی می‌شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of crocin on hormones of pituitary-ovarian axis in type one diabetic rat’s model

نویسندگان [English]

  • T. S. Vafa
  • M. Emadi
Assistant Professor, Department of Animal Science, Faculty of Agriculture, Payam-e-Noor University, Tehran, Iran
چکیده [English]

Diabetes mellitus is a metabolic disease that causes reproductive disorders by dysfunction of the endocrine glands. Due to the role of crocin in modulation of antioxidant defense system, the aim of this study was to determine the effects of crocin on hormones of pituitary-ovarian axis in type one diabetic rat’s model. In this experimental study, 28 wistar female rats were allocated into 4 equal groups. Groups of control, diabetic control and two treated diabetic group with crocin (concentrations of 50 and 100 mg/kg). The diabetes in diabetic control and treated diabetic groups was induced using an intraperitoneal injection of alloxan. Estrous cycle in rats was identical by sex hormones. Crocin was intraperitoneally injected into treated diabetic groups for 25 days. Saline solution was injected to the animals of control and diabetic control groups. At the end of treatment period, the serum levels of LH, FSH, estrogen and progesterone was measured by ELISA. The statistical analysis was carried out using one-way ANOVA and Post Hoc Tukey tests. In comparison with diabetic control group, dose-dependent administration of crocin with concentrations of 50 and 100 mg/kg was significantly increased serum levels of LH, FSH, estrogen and progesterone (p<0.05). Based on results, crocin probably improves endocrine disorders in patients with diabetes by increases serum levels of LH, FSH, estrogen and progesterone.

کلیدواژه‌ها [English]

  • Crocin
  • FSH
  • LH
  • Estrogen
  • Progestrone
  • rat
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