بررسی ارزش تشخیصی آنتی‌ژن B کیست هیداتید، به روش دات بلات در استان آذربایجان شرقی

نوع مقاله: مقاله کامل

نویسندگان

1 گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران

2 بخش پروتئومیکس و بیوشیمی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، ترویج و آموزش کشاورزی، کرج، ایران.

3 گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران.

4 گروه انگل‌شناسی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد گرمسار، سمنان، ایران

5 گروه انگل‌شناسی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد تبریز، تبریز، ایران

6 گروه جراحی توراکس، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران

چکیده

هیداتیدوزیس بعنوان یک بیماری مهم مشترک بین انسان و حیوان مطرح است و در مناطق مختلف جهان، از جمله ایران از شیوع بالایی برخوردار است. روش‌های سرولوژیکی که بتوانند با حساسیت و ویژگی بالا، هیداتیدوز را تشخیص دهند، از ارزش بسیار بالایی برخوردار می‌باشد. در این مطالعه، آنتی‌ژن B مایع کیست هیداتید را جداسازی کرده و بر روی نمونه‌های سرم بدست آمده از انسان‌ها و گوسفندان مبتلا به کیست هیداتید و نمونه‌های سالم، تست دات بلات انجام گرفت تا دقت، حساسیت و ویژگی آن مورد ارزیابی قرار بگیرد. بعد از انجام تمام مراحل تست دات بلات بر روی 200 نمونه جمع‌آوری شده، مشخص شد که دقت این تست در موارد انسانی معادل با 96% و در موارد گوسفندی معادل با 92% می‌باشد. حساسیت این تست در موارد انسانی معادل با 92% و در گوسفندی هم معادل با 84% می‌باشد. ویژگی این تست هم در هر دو مورد معادل 100% بدست آمده است. ارزیابی تمامی این موارد مشخص می‌کند که آنتی‌ژن B، در تشخیص هیداتیدوزیس از ارزش بسیار بالایی برخوردار است و روش دات بلات با استفاده از این آنتی‌ژن، می‌تواند به عنوان یک روش تشخیصی ارزشمند و هم‌چنین بعنوان یک روش غربالگری اولیه مورد استفاده قرار بگیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Diagnostic Value Of Antigen B In Hydatid Cyst By Dot-Blot Technique In The East Azerbaijan Province

نویسندگان [English]

  • salar zarrabi ahrabi 1
  • Rasool Madani 2 3
  • Bahar Shemshadi 1
  • Shahrokh Ranjbar-Bahadori 4
  • hossein Hashemzadeh Farhang 5
  • majid montazer bavili 6
1 Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4 Department of Parasitology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan, Iran
5 Department of Parasitology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
6 Department of Thoracic Surgery, Faculty of Medical Science, Tabriz Medical University, Tabriz, Iran
چکیده [English]

Hydatidosis has been recognized as one of the most important zoonotic diseases in different parts of the world, including Iran. Serological methods that can detect hydatidosis with high sensitivity and specificity are have high value. In this study, the antigen B fluid of hydatid cysts was isolated and Dot-Blot technique performed on serum samples from human and sheep with hydatid cysts and healthy samples to evaluate their accuracy, sensitivity and specificity. After completing all stages of the dot blot test on 200 samples, it was found that the accuracy of the test in human cases is equivalent to 96% and in sheep cases it is equivalent to 92%. The sensitivity of this test is 92% in human cases and 84% in sheep. The test specificity is also 100% in both cases calculated. The evaluation of all of these cases indicates that B antigens have a high value in the diagnosis in hydatidiosis, and the dot blot technique using this antigen can be valuable as a diagnostic method and also as a method initial screening should be used.

کلیدواژه‌ها [English]

  • Hydatid cyst
  • Dot blot
  • Antigen B
  • Echinococcus granulosus
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