تنوع ژنوتیپی ژن گیرنده‌ی شماره‌ی یک دوپامین (DRD1) در مرغ‌های بومی نژادهای مرندی و مازندرانی

نوع مقاله: مقاله کامل

نویسندگان

1 استادیار گروه علوم دامی، واحد مراغه، دانشگاه آزاد اسلامی، مراغه، ایران

2 دانشجوی رشته‌ی ژنتیک، گروه زیست‌شناسی، واحد مراغه، دانشگاه آزاد اسلامی، مراغه، ایران

چکیده

برای شروع و ادامه‌ی کرچی در مرغ‌ها ژن پرولاکتین نقش اساسی دارد. دوپامین در غده‌ی هیپوفیز باعث کاهش اثر پرولاکتین می‌شود. این تحقیق به منظور بررسی چندشکلی‌های‌ آللی ژن گیرنده‌ی شماره‌ی یک دوپامین (DRD1) در مرغ‌های بومی نژادهای مرندی و مازندرانی با استفاده از تکنیک PCR-RFLP انجام شد. به طور تصادفی از 200 قطعه مرغ خون‌گیری و DNAی ژنومی به روش شستشوی نمکی استخراج شد. تکثیر جایگاه ژنی مورد نظر به طول 283 جفت باز به کمک آغازگرهای اختصاصی انجام و برای شناسایی جهش در جایگاه ژنی مورد نظر از آنزیم برشی‌ CfrI استفاده شد. بعد از هضم آنزیمی، برای جایگاه نشانگری DRD1، سه نوع ژنوتیپ AA، AG و GG و دو آلل A (با یک نوار 283 جفت بازی) و G (با دو نوار 183 و 100 جفت بازی) شناسایی شد. توده‌های مرغ‌های بومی از نظر شاخص تعادل برای جایگاه ژنی مورد نظر در تعادل هاردی - واینبرگ قرار داشتند. برای توده‌های مرغ‌های بومی نژادهای مرندی و مازندرانی شاخص اطلاعات شانون در جایگاه نشانگری DRD1 به ترتیب 59/0 و 67/0، شاخص تثبیت به ترتیب 09/0- و 19/0- و مقدار شاخص هتروزیگوسیتی مشاهده ‌شده به ترتیب 44/0 و 58/0 برآورد شدند. با توجه به وجود چندشکلی و جهش در جایگاه ژنی مورد مطالعه و با مطالعه‌ی صفات تولیدی ژنوتیپ‌های مشاهده شده می‌توان از این جایگاه ژنی به ‌عنوان مارکر مناسب در برنامه‌های اصلاح نژاد استفاده کرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Genotypic Diversity Of Dopamine D1 Receptor Gene (DRD1) In Indigenous Chickens Of Marandi And Mazandarani Breeds

نویسندگان [English]

  • Jafar Pish Jang Aghajeri 1
  • Zahra Badakhshan 2
  • Somayeh Tirandaz 2
1 Assistant Professor, Department of Animal Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran
2 Student of Genetics, Department of Biology, Maragheh branch, Islamic Azad University, Maragheh, Iran
چکیده [English]

Prolactin gene plays a key role in the initiation and continuation of broodiness in chickens. Dopamine in the pituitary gland reduces the effect of prolactin. This research was conducted to investigate the allelic polymorphism of dopamine D1 receptor gene (DRD1) using PCR-RFLP method in Marandi and Mazandarani indigenous chickens. In this study, blood samples were collected randomly from 200 chickens and genomic DNA was extracted using salting out method. Amplification of the desired locus with 283 bp was performed using specific primers and the CfrI enzyme was used to identify the mutation in the desired locus. After digestion, for DRD1 marker site, three genotypes AA, AG and GG, and two alleles of A (with on band of 283 bp) and G (with two bands of 183 and 100 bp) were identified. Indigenous chicken masses were in the Hardy-Weinberg equilibrium. For Marandi and Mazandarani indigenous chicken masses, the Shannon information index in DRD1 marker site was 0.59 and 0.67, respectively, the fixation index was -0.09 and -0.19, respectively and the observed heterozygosity index was 0.44 and 0.58, respectively. Regarding the presence of polymorphism and mutation in the studied locus and by studying the traits of related to the observed genotypes, it is suggested that this locus can be used as an appropriate marker for breeding programs.

کلیدواژه‌ها [English]

  • Polymorphism
  • DRD1 gene
  • Broodiness
  • Indigenous chicken
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