ارزیابی ارتباط غلظت سرمی بتاهیدروکسی بوتیرات با شاخص‌های استرس اکسیداتیو، انرژی و ریزمغذی‌ها در شرایط مختلف فیزیولوژیک گاو نژاد هلشتاین

نوع مقاله: مقاله کامل

نویسندگان

1 استاد گروه بیماری‌های درونی و کلینیکال پاتولوژی، دانشکده دامپزشکی دانشگاه ارومیه

2 دانشیار گروه بیماری‌های درونی و کلینیکال پاتولوژی، دانشکده دامپزشکی دانشگاه ارومیه

3 دکتری دامپزشکی، گروه بیماری‌های درونی و کلینیکال پاتولوژی، دانشکده دامپزشکی دانشگاه ارومیه

چکیده

هدف از انجام این پژوهش، بررسی ارتباط بین غلظت سرمی بین بتاهیدروکسی بوتیرات (BHB) با گلوتاتیون پراکسیداز (GPX)، تری‌‌گلیسرید، کلسترول، گلوکز، مس، روی و آهن است. 230 راس گاو از نظر شرایط تولید مثلی به 3 گروه گاوهای آبستن 9-7 ماه، 2 ماه پس از زایش و (ماه آخر شیرواری) انتخاب شدند. دام‌ها از نظر غلظت سرمی BHB، به چهار گروه BHB کمتر از 8/0، 2/1-81/0، 39/1-21/1 و بیشتر از 4/1 میلی‌مول در لیتر طبقه‌بندی شدند. میانگین BHB در تمامی دام‌‌ها 64/0میلی‌‌مول در لیتر بود. مقادیر BHB گاوهای تا 2 ماه پس از زایش، به‌‌طور معنی‌‌داری بیشتر از گاوهای با آبستنی سنگین بود. درصد گاوهای ناسالم 4/17 و شامل BHB بین 2/1-8/0، 4/1-21/1 و 4/1< به‌‌ترتیب 4/10، 6/2 و 4/4 بودند. میانگین غلظت سرمی GPX و روی در بین گروه‌‌های تولیدمثلی و BHB برخلاف تریگلیسرید و کلسترول تفاوت آماری معنی‌‌داری نداشت. پایین‌‌ترین غلظت سرمی مس، روی و آهن در گروه آبستنی سنگین بود. مقادیر سرمی مس و اهن در بین گروه‌‌های تولید مثلی معنی‌دار و براساس گروه‌‌های BHB معنی‌‌دار نبودند. میزان BHB گاوهای آبستن 8/0> و سالم بودند ولی گروه تازه‌زا دارای BHB 8/0< (کتوز تحت‌‌بالینی و بالینی) بودند. هیچ‌‌یک از شاخص‌‌های خون در BHB 8/0< معنی‌‌دار نبودند. BHB با شاخص‌‌های انرژی در گروه‌‌های تولیدمثلی (بجز گلوکز) همبستگی معنی‌‌داری نشان داد. هم‌‌چنین BHB با GPX و مس در گاوهای آبستن و 2 ماه پس از زایش همبستگی معنی داری نشان داد. نتیجه این‌‌که فراوانی کتوز تحت‌‌بالینی حدود چهار برابر بالینی بود. مقدار BHB و شاخص‌‌های انرژی به آبستنی و شیراوری وابستگی داشته اما GPX و ریزمغذی‌‌ها تحت تاثیر BHB نبودند. BHB با GPX و مس در گروه‌‌های تولیدمثلی مرتبط بودند. نهایتاً افزایش BHB با کاهش مس در دوره پس از زایش همراه بوده که می‌‌توانند متاثر از همدیگر باشند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of the correlation of beta hydroxyl butyrate with oxidative stress, energy and trace minerals parameters in dairy cows

نویسندگان [English]

  • A.G. Ramin 1
  • S. Asri-Rezaie 2
  • V. Khorashadizadeh 3
1 Depar. Internal Medicine and Clinical Pathology, Veterinary college, Urmia University, Urmia, Iran.
2 Associate Professor, Depar. Internal Medicine and Clinical Pathology, Veterinary college, Urmia University, Urmia, Iran.
3 Vet Graduated, Veterinary college, Urmia University, Urmia, Iran.
چکیده [English]

The aim of this study was to investigate the relationship between beta hydroxyl butyrate (BHB) with glutathione peroxidase (GPX), triglycerides, cholesterol, glucose, copper, zinc and iron. Based on the reproduction condition, the 230 cows were classified into three groups of 7 to 9 months pregnant cows, 2 months calved cows and from 3 months to the dry period time. Based ob the serum BHB concentration, animals were divided into 4 groups of <0.08, 0.81-1.2, 1.21-1.39 and >1.4 mmol/l. Overall mean BHB was 0.64 mmol/l. BHB in cows calved to 2 months were significantly higher than in pregnant cows. The percentages of unhealthy cows was 17.4 including 10.4% with BHB 0.81-1.2, 2.6% with 1.21-1.39 and 4.4% with >1.4 mmol/l. Mean GPX and zinc was not differed among reproduction and BHB groups but triglyceride and cholesterol were differed. The lowest concentrations of copper, zinc and iron were in pregnant cows. Mean minerals among reproduction groups were significant for copper and iron but among BHB groups were not. BHB in pregnant cows were <0.8 mmol/l and were all health while in calved cows and up to dry period cows have had all types of BHB groups including clinical and subclinical ketosis. None of the blood parameters were significant in BHB >0.8 mmol/l. With the exception of glucose, BHB showed correlations with energy parameters in reproduction and BHB groups. BHB showed correlations with GPX and copper in 2 months cow’s calved and pregnant cows and with zinc in subclinical ketosis group. In conclusion, the subclinical ketosis was 4 folds of clinical ketosis. BHB concentration and energy parameters were depended on pregnancy and parturition positions, while GPX and trace minerals were not dependent. BHB with GPX and copper were correlated in reproduction groups. Finally, increase in serum BHB could accompany by low copper concentrations probably affects each other after post parturition in cows.

کلیدواژه‌ها [English]

  • cow
  • Reproduction
  • BHB
  • GPX
  • ketosis
  • trace minerals
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