تعیین میزان دوز کشنده و دوز موثر در سم مار شاخدار ایرانی

نوع مقاله : مقاله کامل

نویسندگان

1 بخش پروتئومیکس و بیوشیمی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، ترویج و آموزش کشاورزی، کرج، ایران.

2 گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران.

3 دانش‌آموخته بیوشیمی، گروه زیست‌شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

4 استادیار موسسه تحقیقات واکسن و سرم سازی رازی

چکیده

جهت تعیین میزان دوز کشنده (LD50) سم مار شاخدار ایرانی و به دنبال آن تعیین دوز موثر (ED50) پادزهر (آنتی ونوم) آن، ابتدا غلظت پروتئین‌های سم مارشاخدار به روش Lowry و با استفاده از پروتئین استاندارد BSA تعیین گردید که میزان آن برابر با
µg/ml 1500 گزارش گردید. سپس برای اندازه‌گیری میزان کشندگی سم مار، در شش سطح، از موش‌های آزمایشگاهی تیپ BALB/c استرالیایی جنس ماده با وزن gr 20-18 استفاده گردید. همچنین جهت محاسبه نتایج به دست آمده از نرم‌افزار SPSS و برنامه تجزیه آماری Probit استفاده شدکه میزان آن برابر با µg/mouse 9/21 گزارش گردید وبا استفاده از مقدار LD50 به دست آمده و به وسیله مخلوط مقدارثابت سم حل شده در سالین 85 درصد و مقادیر متغیری از آنتی ونوم مونووالان میزان دوزموثر (effective dose) به دست آمد بطوری که ED50 برابر با µg/ml 395/6401 گزارش گردید.با توجه به میزان LD50 به دست آمده، مار شاخدار ایرانی جزء مارهای سمی و خطرناک محسوب می‌شود. ‏با توجه به میزان بالای ED50 بدست آمده، بهتر است از سرم پلی والان برای خنثی‌سازی سم این مار استفاده شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Determination of thelethal dose (LD50) and the effective dose (ED50) of Iranian horned viper venom

نویسندگان [English]

  • R. Madani 1 2
  • S.M. Razavi 3
  • F. Golchinfar 4
1 Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Student of M.Sc. in Biochemistry. Dept. of Biology, School of Basic Sciences, Science & Research Branch, Islamic Azad University, Tehran, Iran.
4 M.Sc. Biochemistry, Biotechnology. Assistant Professor Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IR Iran.
چکیده [English]

To determine the lethal dose (LD50) of Persian horned viper venom and the effective dose of this venom, primarily concentration of proteins in snake venom was measured by Lowry method and using BSA as standard protein which was reported to be 1500 µg/ml. The lethal dose at six levels was calculated on laboratory BALB/c Australian mice and female sex with weight of 18-20 gr, using SPSS software and Probit statistical analysis program, this amount was reported to be 21.9 µg/mouse. While utilizing the obtained LD50 amount and by the fixed amount of compound solved in 85% Saline and varied amounts of monovalent antivenom, the neutralizing dosage or effective dose could be obtained, in such a way that ratio of venom to antivenom was considered at one level and Australian BALB/c mice were injected with 18-20 gr of the female sex at six levels, and the achieved results were reported to be ED50 equal to 6401.4 µg/ml using SPSS software and Probit statistical analysis program. Considering the obtained LD50 amount, Persian horned viper is regarded as one of the poisonous and dangerous snakes. Considering the high obtained ED50 amount, it is better to use the polyvalent serum to neutralize the poson of this snake.

کلیدواژه‌ها [English]

  • protein assay
  • Persian horned viper venom
  • LD50 of Persian snake venom
  • effective dose
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