جداسازی و شناسایی میکروبیولوژیک جدایه‌های پاستورلا مولتوسیدا از گاو در کشتارگاه‌های البرز و تهران

نوع مقاله: مقاله کامل

نویسندگان

بخش واکسن های باکتریایی هوازی دامبزشکی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

چکیده

این مطالعه کشتارگاهی با هدف بررسی حضور پاستورلا مولتوسیدا، جمع‌آوری جدایه‌های آن و همچنین تعیین تیپ کپسولی آن‌ها در یک واحد صنعتی کشتارگاهی در استان البرز (شهرستان کرج) و 5 واحد در استان تهران (رباط کریم‌، شهر ری، شهریار، کهریزک و ورامین) و انجام پذیرفت. تعداد 89 نمونه ریه و 595 سواب از مجاری تنفسی گوساله‌های گاو در یک کشتارگاه در کرج و 5 کشتارگاه در تهران بر روی محیط‌های اختصاصی کشت گردیدند. در نتیجه از مجموع 339 جدایه رشد یافته در کشت تعداد 89 مورد در آزمون‌های متعارف بیوشیمیایی پاستورلا مولتوسیدا شناسایی شدند. اجرای آزمون PM-PCR هویت 64 جدایه از 89 جدایه را به عنوان پاستورلا مولتوسیدا مورد تایید قرار داد. با اجرای آزمون Cap-PCR 60 جدایه دارای تیپ کپسولی A و 1 جدایه نیز دارای تیپ کپسولی B نشان داده شدند در حالی‌که این آزمون نتوانست تیپ کپسولی 3 جدایه را تعیین نماید. نویسندگان معتقدند هنوز تا شناخت کامل اپیدمیولوژی پاستورلا مولتوسیدا در ایران باید تحقیقات بیشتری انجام پذیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Abattoir isolation and microbiological identification of field isolates of Pasteurella multocida collected from cattle at Tehran AND Alborz slaughterhouses

نویسندگان [English]

  • M. Valadan
  • A.R. Jabbari
  • M. Sekhavati
  • M. Niroumand
  • Alireza Arab
  • K. Tadayon
National Research Laboratory of Pasteurella, Veterinary Aerobic Bacteria Vaccines Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.
چکیده [English]

This abattoir-based investigation was conducted in search for presence of Pasteurella multocida, collection of its isolates, characterization of their biochemical profiles and also classification of their capsular types in Tehran and Alborz provinces. Swabs from 89 lungs and 595 nasal passages of bovine calves at a single slaughterhouse in Karaj (Karaj city) and 5 slaughterhouses in Tehran (Shahriar, Shahr-e-Ray, Robat Karim, Kahrizak and Varamin) were cultured on specific media. Among the 339 non-haemolitic individual colonies, 89 were proved P. multocida by conventional biochemical tests. The PM-PCR assay confirmed identity of 64 isolates as Pm where 60 were shown to belong to the capsular type A and a single one to capsular type B. The remaining 3 isolates were non-typeable. We believe still a lot needs to be done at the national level to fully acknowledge the epidemiology of Pm in Iran.

کلیدواژه‌ها [English]

  • Pasteurella multocida
  • calf
  • Capsular type

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