بررسی آلودگی به تیلریا اکویی و بابزیا کابالی در الاغ‌های استان کردستان

نوع مقاله: مقاله کامل

نویسندگان

1 گروه انگل شناسی، دانشکده علوم تخصصی دامپزشکی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران

2 بخش پروتئومیکس و بیوشیمی، موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، ترویج و آموزش کشاورزی، کرج، ایران.

3 گروه میکروبیولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران.

4 گروه اپیدمیولوژی و بیماری‌های مشترک- دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

چکیده

انگل‌های خونی در تک‌سمی‌ها ایجاد بیماری‌هایی را می‌کنند که از مهم‌ترین آن‌ها می‌توان به پیروپلاسموزیس اشاره نمود. پیروپلاسموز در تک‌سمی‌ها بوسیله Theileria equi و Babesia caballi ایجاد شده و دارای گسترش جهانی می‌باشد و به عنوان یک تهدید جدی برای آن‌ها مطرح می‌باشد. در این مطالعه وقوع آلودگی به تک‌یاخته‌های خونی در جمعیت الاغ های استان کردستان با استفاده از روش‌های مشاهده انگل درگسترش مستقیم و جداسازی مولکولی قطعات DNA ژنوم اختصاصی انگل صورت پذیرفت. در این مطالعه تعداد 233 نمونه خون بصورت تصادفی از الاغ‌های استان کردستان جمع‌آوری شد. در بررسی میکروسکوپی گسترش‌های خونی رنگ‌آمیزی شده با گیمسا هیچ موردی از تیلریا اکویی و بابزیا کابالی مشاهده نگردید. بررسی مولکولی قطعات DNA ژنوم نمونه‌های خونی با استفاده از پرایمرهای Tbs-S و Tbs-A نشان داد که علی‌رغم حضور قطعه 401 و 430 جفت باز مربوط به ژنوم بابزیا و تیلریا بر روی ژل آگارز به عنوان شاهد مثبت، هیچ یک از نمونه‌های تحت مطالعه آلودگی به تیلریا اکویی و بابزیا کابالی را نشان نداد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

A survey on the infestation of Theileria equi and Babesia caballi in Donkeys in Kurdestan Province, Iran

نویسندگان [English]

  • S. Kakekhani 1
  • S. Rahbari 1
  • R. Madani 2 3
  • S. Bokaei 4
1 Department of Parasitology and Mycology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4 Department of Epidemiology & Zoonosis, School of Veterinary Medicine, Tehran University, Tehran, Iran.
چکیده [English]

Blood parasites cause the disease in Equine which the most important of them is Piroplasmosis. Equine piroplasmosis is caused by Theileria equi and Babesia caballi, and it has a global expansion which known as an important threat to them. The present study is aimed at detecting piroplasm infection in Donkeys in Kurdestan province, Iran by molecular and microscopic approaches. In this study 233 blood samples, were randomly collected from Donkeys of Kurdestan province. During microscopic examination of Giemsa-stained blood smears, infection with Theileria equi and Babesia caballi was not observed. The Tbs-S/Tbs-A primer set was used for PCR amplification of Theileria equi and Babesia caballi. However , the result indicated that despite the presence of 401 and 430 bp related to the Theileria equi and Babesia caballi genome on the agarose gel as a positive control, none of them was positive for Theileria equi and Babesia caballi on a agarose gel electrophoresis.

کلیدواژه‌ها [English]

  • Theileria equi
  • Babesia cabali
  • Donkey
  • Kurdestan province
  • PCR
1. Alhassan, A., Pumidonming, W., Okamura, M., Hirata, H., Battsetseg, B., Fujisaki, K., Yokoyama, N., Igarashi, I. (2005): Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood. Vet Parasitol 129:43–49
2. Alhassan, A., Govind, Y., Tam, N.T., Thekisoe, O.M., Yokoyama, N., Inoue, N., Igarashi, I. (2007): Comparative evaluation of the sensitivity of LAMP, PCR and in vitro culture methods for the diagnosis of equine piroplasmosis. Parasitol Res 100:1165–1168
3. Ali, S., Sugiomoto, C., Onuma, M. (1996): Equine piroplasmosis. J Equine Sci 7:69–70
4. Avarzed, A., de Waal, D.T., Igarashi, I., Saito, A., Oyamada, T., Toyoda, Y.,Suzuki, N. (1997): Prevalence of equine piroplasmosis in central Mongolia. Onderstepoort J Vet Res 64:141–145
5. Bahrami, S., Ghadrdan, A.R., Mirabdollahi, S.M., Fayed, M.R. (2014): Diagnosis of subclinical equine theileriosis in center of Iran using parasitological and molecular methods. Trop Biomed 31, 110-117.
6. Balkaya I, Utuk A,E, Piskin F,C (2010) Prevalance of Theileria equi and Babesia caballi in Donkeys from Eastern Turkey in Winter Season. Pak Vet J, 30(4): 245-246.
7. Chahan, B., Zhang, S., Seo, J.Y., Nakamura, C., Zhang, G., Bannai, H., Jian, Z., Inokuma, H., Tuchiya, K., Sato, Y., Kabeya, H., Maruyama, S., Mikami, T., Xuan, X. (2006): Seroepidemiological evidence for the possible presence of Babesia (Theileria) equi and Babesia caballi infections in Donkeys in Western Xinjiang, China. J. Vet. Med. Sci. 68(7), 753-755.
8. De Waal, D. T., (2004): Global importance of piroplasmosis. Journal of Protozoology Research; 10: 106-127.
9. De Waal, D.T., 1992. Equine piroplasmosis: A review. British Veterinary Journal 148, 6-14.
10. Friedhoff, K.T., Tenter, A.M., and Muller, I. 1990. Haemoparasites of equines: impact on international trade of horses. Rev. Sci. Tech. Off. Int. Epiz. 9: 1187-1194.10. Hailat, N.Q., Lafi, S.Q.,
11. Laus, F., Spaterna, A., Faillace, V., Veronesi, F., Ravagnan, S., Beribé, F., Cerquetella, M., Meligrana, M. and Tesei, B. (2015): Clinical investigation on Theileria equi and Babesia caballi infections in Italian donkeys. BMC Veterinary Research; 11:100
12. Habibi, Gh., Esmaeilnia, K., Hablolvarid, M.H., Afshari, A., Zamen, M., Bozorgi, S. (2016): Microscopic and Molecular Detection of Theileria (Babesia) equi Infection in Equids of Kurdistan Province, Iran. Iran J Parasitol: Vol. 11, No. 1, pp.86-90
13. Kakekhani, S., Rahbari, S., Madani, R., Bokaei, S.(2017): Molecular and microscopic detection of Theileria equi and Babesia caballi in horses in Kurdestan Province, Iran. Archives of Razi Institute, Vol. 72, No. 1, 51-55
14. Mekibib, B., Manegerew, M., and Tadesse, A. (2010): Prevalence of haemoparasite and associated risk factors in working donkeys in Adiguden and Kwiha districts of Tigray region, Northern Ethiopia. J. Anim. Vet. Adv. 9: 2249-2255.
15. Moretti, A., Mangili, V., Salvatori, R., Maresca, C., Scoccia, E., Torina, A., Moretta, I., Gabrielli, S., Tampieri, M.P., and Pietrobelli, M. (2010) Prevalence and diagnosis of Babesia and Theileria infections in horses in Italy: A preliminary study. Vet. J. 184: 346-350.
16. . Nicolaiewsky, T.B., Richter, M.F., Lunge V.R.(2001):Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction. Vet. Parasitol., v.101, p.9-21
17. Osman, A., El Ghali, A., Salim, B. (2016): Parasitological and Molecular Detection of Equine Piroplasmosis in Horses and Donkeys in South Darfur State, Sudan. Sudan J. Vet. Res. 31: 21-28.
18. Phipps, L.P., Otter, A., (2004):. Transplacental transmission of Theileria equi in two foals born and reared in the United Kingdom. Vet. Rec. 154, 406-408.
19. Preston, P.M. (2001): Theileriosis. In: Service MW (ed) Encyclopedia of arthopod-transmitted infections of man and domestic animals. CABI, Wallingford, pp 487–502
20. Seifi, H.A., Mohria, M., Sardaria, K. (2000): A mixed infection of Babesia equi and Babesia caballi in a racing colt: a report from Iran. J. Equine Vet Sci 20:858–860
21. Soulsby E.J.L.(1982): Helminths, Arthropods and Protozoa of Domesticated Animals. 7th ed. London, UK: Bailliere Tindall; pp. 706–728.
22. Tefera, M., Worku, A., and Tolosa, M.(2011): Prevalence and risk factors for donkey babesiosis in and around Debre Zeit, Central Ethiopia. Vet. Res. 4: 56-60.
23. Uilenberg, G. (2001): Babesiosis. In: Service MW (ed) Encyclopedia of arthropod-transmitted infections of man and domestic animals. CABI, Wallinford, pp 53–60
24. Xuan, X., Igarashi, I., Avarzed, A., Ikadai, N., Inoue, N., Nagasawa, H., Fujisakai, K., Toyoda, Y., Suzuki, N., Milkami, T. (1998): Diagnosis of Babesia caballi infection in horses by polymerase chain reaction. J Protozool Res 8:85–89
25. Zaugg, J.L., Lane, V.M. (1992): Efficacy of buparvaquone as a therapeutic and clearing agent of Babesia equi of European origin in horses. Am J Vet Res 53:1396–1399.