بررسی اثرات ضدمیکروبی عصاره آبی، عصاره اتانولی و اسانس گلپر بر روی سالمونلا تیفی‌موریوم جدا شده از طیور

نوع مقاله : مقاله کامل

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی، گروه بیوانفورماتیک، پژوهشکده زیست‌فناوری کشاورزی، دانشگاه زابل

2 پژوهشکده زیست‌فناوری کشاورزی، دانشگاه زابل

3 گروه گیاهان دارویی، دانشگاه زابل

چکیده

هدف از این مطالعه بررسی اثرات ضدمیکروبی عصاره آبی، عصاره اتانولی و اسانس گلپر بر روی باکتری سالمونلا تیفی‌موریوم (Salmonella typhimurium) جدا شده از طیور بود. عصاره و اسانس گیاه گلپر به ترتیب با استفاده از دستگاه خلاء از مرکز (روتاری) و کلونجر بدست آمد. تعداد 12 سویه S. typhimurium از طیور بومی در شهرستان زابل جداسازی شد. حداقل غلظت بازدارندگی و حداقل غلظت کشندگی عصاره آبی، اتانولی و اسانس گیاه گلپر، با تهیه‌ی غلظت‌های مختلف از آنها به روش رقت‌سازی در چاهک و بررسی اثر غلظت‌های مختلف بر روی رشد باکتری‌ها، تعیین شد. حساسیت سویه‌ها به آنتی‌بیوتیک‌ها با روش استاندارد دیسک دیفیوژن کربی- بائر مورد ارزیابی قرار گرفت. نتایج حاصل از مطالعه حاضر نشان داد که از بین اسانس، عصاره آبی و عصاره اتانولی گلپر، اسانس بیشترین اثر مهارکنندگی را برروی S. typhimurium دارد و همچنین بیشتر سویه‌های S. typhimurium در تمام رقت‌های اسانس گیاهی آن از بین رفته‌اند، در حالی که عصاره آبی کمترین اثر مهارکنندگی را داشته است. بیشترین غلظت مهارکننده اسانس گلپر در رقت ppm 5 بود و عصاره اتانولی در رقت ppm 25 و عصاره آبی در غلظت‌های 50 و ppm 100 مهارکننده بیشتر سویه‌ها بودند. بیشترین غلظت کشندگی نیز برای اسانس، عصاره اتانولی و آبی به ترتیب برابر با 20، 200 و ppm 200 بود. نتایج این مطالعه بیانگر اثرات ضدمیکروبی مناسب اسانس و عصاره اتانلی گلپر بود و می‌توان از آنها به‌عنوان جایگزین آنتی‌بیوتیک‌های متداول، برای درمان عفونت‌های ناشی از این باکتری استفاده کرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Antimicrobial effects of aqueous extract, ethanol extract and essential oil of Golpar on Salmonella typhimurium isolated from poultry

نویسندگان [English]

  • A. Maghsoudi 1
  • A. Maghsoudi 2
  • Seyedeh Razieh Mousavi 3
1 Member of Sientific Board of University of Zabol
2 Center of Agricultural Biotechnology, University of Zabol
3 Department of Medicinal Plants, Faculty of Agriculture, University of Zabol.
چکیده [English]

The aim of this study was to evaluate the antimicrobial effects of aqueous extract, ethanol extract and essential oil of Golpar on Salmonella typhimurium isolates from poultry. Plant extracts and essential oils of Golpar were obtained using vacuum from the center (Rotary) and Clevenger. Total number of 12 S. typhimurium strains were isolated from native poultry in the city of Zabol. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of aqueous and ethanol extract and essential oil of Golpar were determined using preparation of different concentrations by dilution in wells method and determination of different concentrations on growth of bacteria. Susceptibility to several antibiotics was evaluated by the Kirby-Bauer disk diffusion standard method. The results of the present study showed that among the essential oils, aqueous extract and ethanol extract of Golpar, the essence have shown highest inhibitory effect on S. typhimurium, while the aqueous extracts have less inhibitory effects on most of the strains of S. typhimurium. The MIC of essential oil was on 5 ppm dilution but for ethanol extract was on 25 ppm and for aqueous extract was on 50 and 100 ppm. The MBC for essential oil, ethanol and aqueous extracts of Golpar were 20, 200 and 200 ppm, respectively. The results showed relevant antibacterial activity of Golpar extracts and essential oils, which can be used for treatment of infections due to the S. typhimurium, instead of common antibiotics.

کلیدواژه‌ها [English]

  • Antibacterial activity
  • Heracleum persicum
  • Medicinal herbs
  • essential oil
  • Poultry

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  • تاریخ دریافت: 24 بهمن 1395
  • تاریخ بازنگری: 12 مرداد 1396
  • تاریخ پذیرش: 23 مرداد 1396

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