بررسی کارآیی واکسن زنده تخفیف حدت یافته برونشیت عفونی سویه B08IR/ا793 ساخت موسسه رازی

خالصی, بهمن; مسعودی, شهین; ایراهیمی, محمد مجید; پیشرفت ثابت, لیلا

doi:10.22092/vj.2017.114448.1328

بررسی کارآیی واکسن زنده تخفیف حدت یافته برونشیت عفونی سویه B08IR/ا793 ساخت موسسه رازی

نوع مقاله : مقاله کامل

نویسندگان

¹ استادیار بخش تحقیق و تولید واکسن های ویروسی طیور، موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران

² دانشیار بخش تحقیق و تولید واکسن های ویروسی طیور، موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران

10.22092/vj.2017.114448.1328

چکیده

از واکسن‌هایی که در حال حاضر برای کنترل بیماری برونشیت عفونی طیور مورد استفاده قرار می‌گیرد، واکسن حاوی سویه i793/Bمی‌باشد. بدنبال اثبات حضور ویروس i793/B در اکثر مرغداری‌های کشور واکسن زنده تخفیف حدت‌یافته برونشیت عفونی سویه i793/B081R، در موسسه رازی تولید شد. هدف از انجام این پروژه ارزیابی کارآیی واکسن i793/B081Rبر اساس پروتکل OIE با استفاده از ارزیابی فعالیت مژک‌های نای (Ciliary activity) پرندگان مایه‌کوبی شده بعد از چالش با ویروس حاد بود. نتایج بررسی حرکت مژک‌های نای پس از چالش با ویروس حاد در گروه‌های رازی، وارداتی و شاهد بترتیب 5/1، 7/1 و 4 بود. طبق استاندارد امتیاز کمتر یا مساوی 2 نشان‌دهنده مصونیت پرنده مایه‌کوبی شده در مقابل ویروس حاد می‌باشد و امتیاز بیشتر از 2 نشان‌دهنده عدم مقاومت پرنده مایه‌کوبی شده است. نتایج بدست‌آمده موید این نکته بود که واکسن رازی در مقایسه با واکسن وارداتی از کارآیی بالایی برخوردار است و 100 درصد پرندگان مایه‌کوبی شده در مقابل ویروس حاد مقاومت نمودند درحالی‌که تمامی پرندگان گروه شاهد توقف کامل مژک‌های نای را بدون هیچ‌گونه مقاومت در برابر ویروس حاد نشان دادند. همچنین آزمایشات سرولوژی (ELISA) و نوترالیزاسیون سرم (SN (Serume Neutralization افزایش قابل توجه تیتر سرمی در مقایسه با گروه شاهد نشان دادند.

کلیدواژه‌ها

20.1001.1.24235407.1397.31.1.5.6

موضوعات

ویروس‌شناسی دامپزشکی

عنوان مقاله [English]

Efficacy evaluation of Razi Infectious Bronchitis Live Attenuated Vaccine 793/B08IR Strain

نویسندگان [English]

B. Khalesi ¹
Sh. Masoudi ²
M.M. Ebrahimi ¹
L. Pishraft-Sabet ¹

¹ Assistant Professor, Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

² Associate Professor,Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

چکیده [English]

Vaccines that are currently used to control infectious bronchitis are vaccines containing 793/B strain. Following the evidence of 793/B viral infection in most of the country's poultry farms, a live vaccine for virulent infectious bronchitis strain 793/B08IR was developed at the Razi Institute. The purpose of this project was to evaluate the efficacy of vaccine 793/B08IR based on the OIE protocol by assessing the activity of ciliary activity of IBV vaccinated birds after the challenge of acute viruses. The results of the study of trachea after the challenge of acute virus in the Razi, imported and control groups were 1.5, 1.7 and 4, respectively. According to the standard, a score of less than or equal to 2 indicates the immunity of the bird vaccinated against the acute virus, and a score of more than 2 indicates the lack of resistance of the bird. The results indicated that Razi vaccine was highly effective compared to the imported vaccine, and 100% of the birds vaccinated against the acute virus resisted, while all the birds in the control group stopped full trachea without any resistance acute virus showed. Serological tests (ELISA) and serum neutralization (SN) also showed a significant increase in serum titer compared to the control group.

کلیدواژه‌ها [English]

vaccine
Infectious bronchitis 793/B Serotype
Cilliostasis

مراجع

1- Aghahakn, S. M., Afshar Fereidouni, N.A., Marunesi, C., Khodashenas, M. 1994. Studies on avian viral infection in Iran. Archives of Razi Institute 45: 1-10.
2- Akbari Azad, G., Vasfi Marandi, M., Keyvani aminae, H. 2007. Molecular analysis of three iranian isolates belonged to 793/B serotype of infectious bronchitis viruses. Journal of Veterinary Research 62,1:69-80.
3- Cavanagh, D., Ellis, M. M., Cook, J. K. A. 1997. Relationship between variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection In vivo. Avian pathology 26: 63-74.
4- Cavanagh, D., Naqi, S.A. 2003. Infectious Bronchitis; in Disease of Poultry, Saif, Y.M ., Barnes, H. J., Glisson, J.R Fadly, A.M., McDougald, L R. and Swayne, D.E. (eds Iowa State University Press, Amen. pp: 101-119.
5- Cavanagh, D. 2007. Coronavirus avian infectious bronchitis virus. Veterinary Research 38: 281-97.
6- Cavanagh, D. & Gelb, J. 2008. Infectious bronchitis. In Y.M. Saif, A. M. Fadly, J.R. Glisson, L.R. McDougald, L.K. Nolan & D. E. Swayne (Eds.). Diseases of Poultry 12thedn (pp. 117-135). Ames, Iowa, USA: Blackwell Publishing Professional.
7- Cook, J. K. A., Orbell S. J., Woods, M. A., Huggins, M. B. 1996. A survey of the presence of a new infectious bronchitis virus designated 4/91 (793B). The Veterinary Record 138:178–180.
8- Cook, J. K. A., Orbell, S.Y., Woods, M.A., Huggings, M.B. 1999. Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with Infectious Bronchitis Virus of heterologous serotypes. Avian Pathology 28:477-485.
9- Cook, J. K. A., Ashesher J., Baxendal, W., Green Wood, N., Huggins, M. B., Orbell, S. J. 2001. Protection of chickens against renal damage caused by a nephropathogenic Infectious Bronchitis Virus. Avian Pathology 30: 423-425.
10- Cubillos, A., Ulloa, J., Cubillos, V., Cook, J. K. A. 1991. Characterisation of strains of infectious bronchitis virus isolated in Chile. Avian Pathology 20: 85-99.
11- Darbyshire J.H. (1985). A clearance test to assess protection in chickens vaccinated against avian infectious bronchitis virus. Avian Pathology 14, 497–508.
12- Dawson., P. S., Gough., R. E. 1971. Antigenic variation in strains of avian infectious bronchitis virus. Archiv für die gesamte Virusforschung 34, 32–39.
13- European Pharmacopoeia ,8th Edition, Avain infectious bronchitis vaccine (Live): 04/2313:0442.PP: 926-928. Available on:http://www.fptl.ru/biblioteka/farmakopei/evropeyskaya_farmakopeya_8_vol-1.PDF.
14- Geerligs, H. J., Boelm, G. J., Meinders, C. A. M., Stuurman, B. G. E., Tarres-Call, J., Bru ,T., Vila, R., Mombarg, M., Karaca, K., Symons, J., Wijmenga, W. and M. Kumar.2011.Efﬁcacy and safety of an attenuated live QX-like infectious bronchitis virus strain as a vaccine for chickens. Avian Pathology 40(1) 93_102.
15- Ghahremani, N., Bozorgmehri Fard, M. H., Shoushtari, H., Momayez, R., Sheikhi, N, Khoshzahmat, A., Eshratabadi, F. 2011. Molecular analysis of Infectious Bronchitis Virus isolated in Iran from 1998-2008. Journal of Animal and Veterinary Advances 10: 2961-2967.
16- Gholami Ahangaran, M., Shoushtari, A. H., Doosti, A., Fathi Hafshejani, E., Zia-Jahromi, N. 2012. Detection of Infectious Bronchitis Virus (4/91 type) in Broiler Chickens in Chahrmahal-va-bakhtiyari Province. Veterinary Clinical Pathology (Veterinary Journal of Tabriz) 22:1543-1547.
17- Gough, R. E., Randall, C.J., Dagless, M., Alexander, D.J., Cox, W. J., Pearson, D. 1992 . A 'new' strain of infectious bronchitis virus infecting domestic fowl in Great Britain. Veterinary Research 30: 493-4.
18- Hosseini, H., Akbari, G., Bahonar., A. 2014. Evaluation of the efficacy of BRONIPRA-I infectious bronchitis live vaccine in broiler chickens. Research project (In Persian).
19- Hosseini Aliabadi A.O, Momayez, R., Mahmoudzadeh, M., Yousefi Amini, A. 2013. Detection of 793/B serotype in broiler chicken flocks with respiratory out breaks in west of Mazandaran. Journal of veterinary clinical research 2: 91-97. (In Persian).
20- Kant, A., Koch, G., van Roozelaar, D. J., Kusters, J.G., Poelwijk, F.A.J., van der Zeist, B. A. M. 1992. Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis glycopeptide. Journal of general virology 73:591-596.
21- Lai, M. M. C., Holmes, K.V. 2001. Coronaviridae: the viruses and their replication. In: Knipe, D.M., Howley, P.M., Grifﬁn, D.E., Lamb, R.A., Martin, M.A., Roizman, B., Straus, S.E. (Eds.), Fields Virology, fourth ed. Lippincott Williams & Wilkins, Philadelphia, PA, pp. 1163–1185.
22- Momayes, R., Pourbakhsh, S. A., Khodashenas, M., Banani, M. 2002. Isolation and Identification of infections bronchitis virus from commertial chickens. Archives of Razi Institute 53:1-10.
23- Nouri, A., Assasi, K., Seyfi-Abad Shapouri, M. R. 2003. Field study of Infectious Bronchitis Virus in broiler using type-specific RT-PCR. Archives of Razi Institute 55: 1-10.
24- OIE (Office international des epizooties). Manual of standards for Diagnostic tests and Vaccine. (Avian Infectious Bronchitis) Seventh Edition. Chapter 2.3.2: 414-426. 2012. Available on: http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm /2.03.02_AIB.PDF.
25- Roser, D., Pujols, J., Ordonez, G., Porta, R., Majo, N. 2008 .Molecular epidemiology and evolution of avian infectious bronchitis virus in Spain over a fourteen-year period. Virology 1: 50-59.
26- Seify Abad Shapouri, M. R., Mayah, M., Charkhkar, S., Assasi K. 2002. Serotype identification of recent Iranian isolates of Infectious Bronchitis Virus by type-specific multiplex RT-PCR. Archives of Razi Institute 53: 79-85.
27- Shirzad, M. R., Asasi. K., Mohammadi. A. 2012. Efficacy of vaccination programmes using two commercial live infectious bronchitis vaccines against a field IRFIB 32 strain. Bulgarian Journal of Veterinary Medicine 4: 260 - 272.
28- Shoushtari, A.H., Toroghi, R., Momayez, R., Pourbakhsh, S. A. 2008.793/B type the predominant circulating type of avian Infectious Bronchitis Viruses 1999-2004 in Iran: a retrospective study. Archives of Razi Institute 63: 1-5.
29- Tarpey, I., Orbell, S. J., Britton ,P., Casais, R., Hodgson, T., Lin ,F., Hogan, E., Cavanagh, D. 2006. Safety and efficacy of an infectious bronchitis virus used for chicken embryo vaccination. Vaccine 24 6830 – 6838.
30- Terregino, C., Toffan, A., Beato, M.S., Nardi, R.D., Vescellari, M., Meini, A., Ortali, G., Mancin, M., Capua, I. 2008. Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4-91 serotype. Avian Pathology 37: 487-493.
31- Valinenejad , A., Kalidari,. M., Akhavizadegan,. M. A, Vahadi, F., Pourbakhsh,A. 2001. Field trial of infectious Bronchitis Vaccine (H120) in broiler flocks of Mashhad (research project) (In Persian).
32- Vasfi Marandi, M., Bozorgmehri Fard, M. H. 2000. Isolation and identification of infectious bronchitis viruses in chickens in Iran. Proceedings of the World’s Poultry Congress PP: 20-25. Montreal, Canada/ August.
33- Williams, A. K., Wang, L., Sneed, L.W., Collisson, E.W. 1993. Analysis of a hypervariable region. In the 3' noncoding end of the infectious bronchitis virus genome. Virus Research 28: 19-27.
34- Zhao, Y., Cheng, J, L., Liu,. X. Y, Zhao, J., Hu, Y. X., Zhang, G. Z. 2015. Safety and efficacy of an attenuated Chinese QX-like infectious bronchitis virus strain as a candidate vaccine. Veterinary Microbiology 22; 180 (1-2):49-58.