ارزیابی واریته تخفیف حدت یافته نئوسپورا کنینوم در تشخیص عفونت نئوسپورا کنینوم در گاو با روش آگلوتیناسیون

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی دانشگاه آزاد واحد جهرم

2 عضو هیات علمی موسسه تحقیقات واکسن و سرم‌سازی رازی شعبه شیراز، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

چکیده

تک یاخته نئوسپورا کانینوم (Neospora caninum) عامل نئوسپوروزیس در میزبان‌های مختلف از جمله گاو، گوسفند، بز،گوزن، اسب، سگ، گربه و موش می‌باشد. پاساژ متوالی نئوسپورا کانینوم ثابت شده است که روی حدت و بیماریزایی آن تاثیر بسزایی دارد اما اینکه آیا این رویه روی ارزش تشخیصی واریته کاهش حدت یافته نیز تاثیر دارد موضوعی است که این مطالعه سعی در بررسی آن دارد لذا هدف از این مطالعه، ارزیابی ارزش تشخیصی واریته تخفیف حدت نئوسپورا کنینوم با استفاده از روش آگلوتیناسیون در تشخیص نئوسپوروزیس در گاو می‌باشد. برای دستیابی به این هدف نمونه‌های سرمی 184 عدد گاو از دامداری‌های استان فارس که دارای سابقه سقط طی یک سال گذشته بودند، جمع‌آوری شد. نمونه‌ها برای تشخیص آنتی‌بادی علیه نئوسپورا کنینوم با کیت تجاری الیزا و روش آگلوتیناسیون با استفاده از تاکی زوایت تهیه شده از واریته تخفیف حدت یافته بررسی شدند. نتایج این مطالعه نشان داد که 54 (34/29%) و 59 (07/32%) سرم به ترتیب با روش آگلوتیناسیون و کیت تجاری الیزا مثبت تشخیص داده شدند .ارزیابی آماری نشان داد که تفاوت معنی‌داری بین این دو روش وجود ندارد و براساس آزمون کاپا توافق این دو تست بسیار خوب بود. این مطالعه ثابت کرد که تک یاخته نئوسپورا تخفیف حدت داده شده از طریق پاساژ متوالی همچنان ارزش تشخیصی خود را در روش آگلوتیناسیون حفظ می کند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of attenuated variety of Neospora caninum for diagnosis of infection in cattle by agglutination test

نویسندگان [English]

  • H.R. Tavanaee 1
  • Mehdi Namavari 2
1 Student, Azad University of Jahrom Branch
2 Member of Board Scientific Razi Vaccine and Serum Research Institute, Shiraz Branch, Agricultural Research, Education and Extension Organisation (AREEO), Tehran, Iran.
چکیده [English]

The protozoan parasite Neospora caninum is a major cause of abortion in cattle worldwide. It has been found in various animal species such as dogs, cattle, horses, sheep and goats. This is conclusive proof that long-term passage of tachyzoites in tissue culture can attenuate virulence of Neospora caninum in vivo. But whether the attenuation procedure could reduces the diagnostic value of attenuated variety is the issue of the impact that this study tries to evaluate it. In other word the aim of this study was to evaluate the attenuated variety of Neospora caninum for diagnosis of neosporosis in cattle by agglutination method. The study was conducted with 184 sera collected from cattle with a history of abortion during the past year from the farms of Fars province. The sera were tested for the detection of antibodies against Neospora caninum with commercial ELISA kit and agglutination method. The results of this study showed that the positivity rate for the sera was 54 (29.34%) for agglutination method compared to 59(32.07 %) obtained by ELISA kit. The difference of agglutination method with ELISA kits according to the McNemar test was not significant and this result was also very good agreement with the Kappa test. This study proved that the attenuated variety of Neospora caninum still preserves its own diagnostic value.

کلیدواژه‌ها [English]

  • Neospora caninum
  • Agglutination test
  • Cattle
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